[논문]구강세정제가 다중 구강 바이오필름 모델에 미치는 영향

Soo-Kyung Jun; Young-Suk Choi

doi:10.13065/jksdh.20230023

구강세정제가 다중 구강 바이오필름 모델에 미치는 영향
Effects of antibacterial mouth rinses on multiple oral biofilms model 원문보기

JKSDH : Journal of Korean Society of Dental Hygiene = 한국치위생학회지, v.23 no.4, 2023년, pp.209 - 218

Soo-Kyung Jun (Department of Dental Hygiene, Hanseo University) , Young-Suk Choi (Department of Dental Hygiene, Shinsung University)

초록
AI-Helper

연구목적: 실험실내에서 성분이 다른 구강세정제가 오래 형성된 다중 구강 바이오필름에 미치는 항균효과를 확인하였다. 연구방법: 구강세정제의 항균효과를 ATP, CFU로 확인하였고, 다중 구강 바이오필름을 형성시킨 sHA 디스크를 구강세정제로 처리한 후 SEM을 이용하여 형태학적 모양을 관찰하였다. 총RNA는 P. intermedia의 바이오필름에서 추출하였고 RNA sequencing 분석을 하였다. 연구결과: 대조군과 구강세정제 4개를 비교한 CFU 측정 결과에서 오래 형성된 다중 구강 바이오필름은 39.0%로부터 95.7%까지 감소한 것으로 나타났다(p<0.05). 에센셜 오일 처리 후 구강생물막 세포들은 크기가 변하고 일부 파열되고 작은 세포 파편 조각도 나타났다. 에센셜 오일로 처리 후 P. intermedia 유전자 발현은 RNA 전사와 단백질 번역 관련 유전자에서 유의한 변화를 보였다. 결론: 성분이 다른 구강세정제는 항균효과를 가지며 표면 구조 및 유전자 발현에 영향을 미친다.

Abstract ▼ AI-Helper

Objectives: To confirm the antibacterial effects of each mouth rinse on multiple oral biofilms in vitro. Methods: The antibacterial effects of different mouth rinses were examined by ATP and counted colony forming units (CFU). Preformed oral biofilms on saliva coated hydroxyapatite (sHA) disks were treated with essential oil and saline; then, the multiple oral biofilms were observed by Scanning electron microscope (SEM). RNA sequencing analysis was performed on total RNA isolated from old biofilms of P. intermedia ATCC 49046. Results: In the CFU measured result compared to controls, preformed multiple oral biofilms were reduced from a low of 39.0% to 95.7% (p<0.05). The size of bacterial cells changed after treatment with the essential oil, and some of the cells ruptured into small pieces of cell debris. Gene expression in P. intermedia ATCC 49046 significantly altered in RNA transcribed and protein translated genes after exposure to essential oil. Conclusions: Mouth rinse solutions with different ingredients had different antibacterial effects and may alter surface structure and gene expression as determined by RNA sequencing.

주제어

표/그림 (5)

표 Table 1. Ingredient and pH of mouth rinse solution
그림 Fig. 1. Viability of remaining multiple oral biofilms was determined by ATP and expressed in relative light units (Luminence). ATP-based luminescence quantification was performed in at least two independent experiments and expressed as the mean (SD) percent relative light units (RLU) versus saline-treated biofilms (Control). Different letters indicate significant differences among the biofilm conditions (p <0.05). *Image is numbering 1: EO, 2: BC, 3: DPZ, 4: CHX, 5: Saline
그림 Fig. 2. Effect of mouth rinsing solution on the viability in multiple oral biofilms CFU was performed in at least three independent experiments and expressed as the mean (SD) percent colony form uint (CFU) versus saline-treated biofilms (Control). Different letters indicate significant differences among the biofilm conditions (p <0.05). *Image is numbering 1: EO, 2: BC, 3: DPZ, 4: CHX, 5: Saline
그림 Fig. 3. SEM images of multispecies oral biofilms were treated with saline (A) and EO (B) then observed using SEM at a magnification of 100,000×(inset) operating at 10 kV.
그림 Fig. 4. RNA sequencing results. The mouth rinse co-culture group (T) showed a significant difference between control group (C). (A) Clustering heatmap comparing the fold changes of genes between experimental groups; (B) A scatterplot to assess manifestation of the experimental control groups; (C) A chart showing the percentage and number of genes; the expression of each gene significant changed (below two images). *Abbreviations: (T) Treat group, (C) Control group

참고문헌 (30)

Paster BJ, Olsen I, Aas JA, Dewhirst FE. The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol 20002006;42(1):80-7.https://doi.org/10.1111/j.1600-0757.2006.00174.x？

상세보기
Kidd EAM, Fejerskov O. What constitutes dental caries? Histopathology of carious enamel and dentin related to the action of cariogenic biofilms. J Dent Res 2004;83(suppl1):C35-8. https://doi.org/10.1177/154405910408301s07？

상세보기
Marsh PD. Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 2016;8(2):263-71. https://doi.org/10.1177/08959374940080022001？

상세보기
Benoit S, Christine R, Pierre P, Danielle D. Role of Fusobacterium nucleatum in periodontal health and disease. Curr Issues Mol Bio 2011;13(2):25-36. https://doi.org/10.21775/cimb.013.025？
Yano A, Kikuchi S, Takahashi T, Kohama K, Yoshida Y.Inhibitory effects of the phenolic fraction from the pomace of vitis coignetiae on biofilm formation by Streptococcus mutans. Arch Oral Biol 2012;57(6):711-9. https://doi.org/10.1016/j.archoralbio.2012.01.001？

상세보기
Cheng L, Weir MD, Xu HHK, Kraigsley AM, Lin NJ, Lin-Gibson S, et al. Antibacterial and physical properties of calcium-phosphate and calcium-fluoride nanocomposites with chlorhexidine. Dent Mater 2012;28(5):573-83. https://doi.org/10.1016/j.dental.2012.01.006？

상세보기
Compton FH, Beagrie GS. Inhibitory effect of benzethonium and zinc chloride mouthrinses on human dental plaque and gingivitis. J Clin Periodontol 1975;2(1):33-43. https://doi.org/10.1111/j.1600-051x.1975.tb01724.x？

상세보기
Ross NM, Mankodi SM, Mostler KL, Charles CH, Bartels LL. Effect of rinsing time on antiplaque-antigingivitis efficacy of listerine. J Clin Periodontol 1993;20(4):279-81. https://doi.org/10.1111/j.1600-051x.1993.tb00358.x？

상세보기
Laine P, Meurman JH, Murtomaa H, Lindqvist C, Torkko H, Pyrhonen S, et al. One-year trial of the effect of rinsing with an amine fluoride-stannous-fluoride-containing mouthwash on gingival index scores and salivary microbial counts in lymphoma patients receiving cytostatic drugs. J Clin Periodontol 1993;20(9):628-34. https://doi.org/10.1111/j.1600-051x.1993.tb00707.x？

상세보기
Collins JR, Olsen J, Cuesta A, Maria SV, Hernandez M, Romanos G, et al.In vitro microbiological analysis on antibacterial, anti-inflammatory, and inhibitory action on matrix metalloproteinases-8 of commercially available chlorhexidine digluconate mouth rinses. Indian J Dent Res 2018;29(6):799-807. https://doi.org/10.4103/ijdr.IJDR_406_17？

상세보기
Wakabayashi H, Yamauchi K, Kobayashi T, Yaeshima T, Iwatsuki K, Yoshie H. Inhibitory Effects of lactoferrin on growth and biofilm formation of Porphyromonas gingivalis and Prevotella intermedia. Antimicrob Agents Chemother 2009;53(8):3308-16. https://doi.org/10.1128/AAC.01688-08？

상세보기
Clark WB, Bammann LL, Gibbons RJ. Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun 1978;19(3):846-53. https://doi.org/10.1128/iai.19.3.846-853.1978？

상세보기
Alvarez J, Smyth DR. CRABS CLAW and SPATULA, two Arabidopsis genes that control carpel development in parallel with AGAMOUS. Development 1999;126(11):2377-86. https://doi.org/10.1242/dev.126.11.2377？

상세보기
Gentleman R, Carey V, Bates D, Bolstad B, Dettling M, Dudoit S, et al. Bioconductor: open software development for computational biology and bioinformatics. Genome Biol 2004;5(10):R80. https://doi.org/10.1186/gb-2004-5-10-r80？

상세보기
Zijnge V, van Leeuwen MBM, Degener JE, Abbas F, Thurnheer T, Gmur R, et al. Oral biofilm architecture on natural teeth. PLoS One 2010;5(2):e9321. https://doi.org/10.1371/journal.pone.0009321？

상세보기
Ian K, Paul WL, David AR. Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci 1999;96(25):14547-52. https://doi.org/10.1073/pnas.96.25.14547？

상세보기
Charles CH, Sharma NC, Galustians HJ, Qaqish J, McGuire JA, Vincent JW. Comparative efficacy of an antiseptic mouthrinse and an antiplaque/antigingivitis dentifrice: a six-month clinical trial. J Am Dent Assoc 2001;132(5):670-5. https://doi.org/10.14219/jada.archive.2001.0245？

상세보기
Mandel ID. Chemotherapeutic agents for controlling plaque and gingivitis. J Clin Periodontol 1988;15(8):488-98. https://doi.org/10.1111/j.1600-051x.1988.tb01020.x？

상세보기
Kasuga Y, Ikenoya H, Okuda K. Bactericidal effects of mouth rinses on oral bacteria. Bull Tokyo Dent Coll 1997;38(4):297-302.？

상세보기
Fine DH, Furgang D, Barnett ML, Drew C, Steinberg L, Charles CH, et al. Effect of an essential oil-containing antiseptic mouthrinse on plaque and salivary Streptococcus mutans levels. J Clin Periodontol 2000;27(3):157-61. https://doi.org/10.1034/j.1600-051x.2000.027003157.x？

상세보기
Shim JY, Yim SB, Chung JH, Hong KS. Antiplaque and antigingivitis effects of a mouthrinse containing cetylpyridinium chloride, triclosan and dipotassium glycyrrhizinate. J Periodontal Implant Sci 2012;42(2):33-8. https://doi.org/10.5051/jpis.2012.42.2.33？

원문보기 상세보기
Takehiro O, Junji TB Khairul MC. Potentials of mouthwashes in disinfecting cariogenic bacteria and biofilms leading to inhibition of caries. OpenDent J2012;(6):23-30. https://doi.org/10.2174/1874210601206010023？

상세보기
Charles CH, Mostler KM, Bartels LL, Mankodi SM.Comparative antiplaque and antigingivitis effectiveness of a chlorhexidine and an essential oil mouthrinse: 6-month clinical trial. J Clin Periodontol 2004;31(10):878-84. https://doi.org/10.1111/j.1600-051X.2004.00578.x？

상세보기
Ya S, Sonja S, Markus H. Antimicrobial efficacy of chlorhexidine against bacteria in biofilms at different stages of development. J Endod 2011;37(5):657-61.https://doi.org/10.1016/j.joen.2011.02.007？

상세보기
Chang YC, Huang FM, Tai KW, Chou MY. The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;92(4):446-50. https://doi.org/10.1067/moe.2001.116812？

상세보기
Giannelli M, Chellini F, Margheri M, Tonelli P, Tani A. Effect of chlorhexidine digluconate on different cell types: a molecular and ultrastructural investigation. Toxicol In Vitro 2008;22(2):308-17. https://doi.org/10.1016/j.tiv.2007.09.012？

상세보기
Marzena WS, Kotwicka M, Urbaniak P, Nowak A, Ewa SJ, Jankun J. Clinical implications of the growth-suppressive effects of chlorhexidine at low and high concentrations on human gingival fibroblasts and changes in morphology. Int J Mol Med 2016;37(6):1594-600. https://doi.org/10.3892/ijmm.2016.2550？

상세보기
Luiz FNPC, Cristine SFA, Davi SB, Anna TTL, Mariana FF. Chlorhexidine mouthwash as an adjunct to mechanical therapy in chronic periodontitis: a meta-analysis. J Am Dent Assoc 2017;148(5):308. https://doi.org/10.1016/j.adaj.2017.01.021？

상세보기
Van LMPC, Slot DE, Weijden GA. Essential oils compared to chlorhexidine with respect to plaque and parameters of gingival inflammation: a systematic review. J Periodontol 2011;82(2):174-94. https://doi.org/10.1902/jop.2010.100266？

상세보기
Jelenaa M, Draganab R, Biljanad N, Tatjanad M, Stevoe M, Dejanf M. EO-based mouthwashes: Is there something that should be known? Balkan J Dent Med 2023;23(1):32-7. https://doi.org/10.5937/bjdm2301032M

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증