[논문]자어기 넙치(Paralichthys olivaceus)의 사육 밀도에 따른 기형 발생과 스트레스 및 면역 유전자 발현 분석

이상현; 박종원; 정민환; 정효선; 김주란; 김우진; 이정호; 이다인

doi:10.5657/kfas.2023.0380

자어기 넙치(Paralichthys olivaceus)의 사육 밀도에 따른 기형 발생과 스트레스 및 면역 유전자 발현 분석
Effects of Rearing Density Stress on Malformation and Stress and Immune Related Gene Expression of Juvenile Olive Flounder Paralichthys olivaceus 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.56 no.4, 2023년, pp.380 - 387

이상현 (국립수산과학원 양식산산업연구부 육종연구센터) , 박종원 (국립수산과학원 양식산산업연구부 육종연구센터) , 정민환 (국립수산과학원 양식산산업연구부 육종연구센터) , 정효선 (국립수산과학원 양식산산업연구부 육종연구센터) , 김주란 (국립수산과학원 양식산산업연구부 육종연구센터) , 김우진 (국립수산과학원 양식산업연구부 생명공학과) , 이정호 (국립수산과학원 양식산산업연구부 육종연구센터) , 이다인 (국립수산과학원 양식산산업연구부 육종연구센터)

Abstract ▼ AI-Helper

Stocking density is associated with Paralichthys olivaceus growth; thus, fish should be rapidly reared at high densities for commercial reasons. Studies have reported that high stocking density retards growth; however, few have investigated the malformations caused by stocking density stress. This study compared the growth and malformation rates of P. olivaceus at different densities and stress- and immune-related gene expression between malformed and normal fish. Forty days post-hatching, fish (total length, 1.49±0.02 cm) were reared at 800 (low density; LD), 1500 (medium density; MD), and 4000 (high density; HD) fish/m2, and the growth rate was measured weekly. On day 30, RNA was extracted from the kidneys, and the expression of stress-, immune-, and malformation-related genes was analyzed using qRT-PCR. The malformation rate in the HD groups was approximately three times higher (62%) than that in the LD and MD groups (approximately 20%), and growth was lower regarding length and weight. The stress-related (HSP70 and GPX) and immune-related (PIR and IgM) genes showed higher mRNA expression in the HD group and malformed fish than in the LD group and normal fish. However, TLR3 showed the opposite results. In summary, high stocking density suppressed growth and increased malformation risk in P. olivaceus.

주제어

표/그림 (7)

그림 Fig. 1. (a), (b) and (c) show the results of identification of deformed fish by reared for 30 days in different densities. And deformed fishes are marked with red circles. Figures (d) and (e) show the criteria for distinguishing between normal and deformed fishes. LD, Low density; MD, Medium density; HD, High density; NO, Normal; DE, Deform.
표 Table 1. Oligonucleotide primers used for quantitative real-time PCR
그림 Fig. 2. Expressions of Stress related and Antioxidative stress genes in the kidney of olive flounder Paralichthys olivaceus. Values (means of triplicate±SD) in the same column sharing the same superscript letter are not significantly different (P<0.05). A, Reared under different stocking densities for 30 days. B, Normal or deformed olive flounder of HD group. LD, Low density; MD, Medium density; HD, High density; NO, Normal; DE, Deformed; GPX, Glutathione peroxidase; HSP70, Heat shock protein 70.
표 Table 2. Covering rate of olive founder Paralichthys olivaceus reared under different stocking densities for 30 days
그림 Fig. 3. Expressions of immune related genes in the kidney of olive flounder Paralichthys olivaceus. Values (means of triplicate±SD) in the same column sharing the same superscript letter are not significantly different (P<0.05). A, Reared under different stocking densities for 30 days. B, Normal or deformed olive flounder of HD group. LD, Low density; MD, Medium density; HD, High density; NO, Normal; DE, Deformed; PIR, Polymeric Ig receptor; IgM, Immunoglobulin M; TLR3, Toll-like receptor 3.
표 Table 3. Mean length of olive flounder Paralichthys olivaceus reared under different stocking densities for 30 days (P<0.05)
표 Table 4. Deformity rate and growth performance of olive flounder Paralichthys olivaceus reared under different stocking densities for 30 days

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