[논문]뜸부기(Silvetia siliquosa) 추출물의 항노화 및 항치매 활성

최지원; 이연지; 김원석; 문수경; 김용태

doi:10.5657/kfas.2023.0526

뜸부기(Silvetia siliquosa) 추출물의 항노화 및 항치매 활성
Anti-aging and Anti-dementia Activities of Different Solvent Extracts from Silvetia siliquosa 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.56 no.4, 2023년, pp.526 - 531

최지원 (군산대학교 식품생명공학전공) , 이연지 (군산대학교 식품생명공학전공) , 김원석 (신라대학교 제약공학과) , 문수경 (경상국립대학교 식품영양학과) , 김용태 (군산대학교 식품생명공학전공)

Abstract ▼ AI-Helper

This study investigated the ingredients, anti-aging and anti-dementia activities of the Korean marine algae Silvetia siliquosa. The S. siliquosa solvent extracts were prepared with 70% ethanol, 80% methanol, and distilled water. The extraction yield range of various solvent extracts was 15.82-49.98%. The ethanol and methanol extracts had higher tyrosinase and collagenase inhibitory activities than those of the water extract. Meanwhile, all extracts exhibited high elastase inhibitory activity. Conversely, the methanol and water extracts exhibited the highest acetylcholinesterase inhibitory activity (IC50, 0.40 mg/mL) and β-secretase inhibitory activity (IC50, 0.81 ㎍/mL), respectively. These results indicate that S. siliquosa may be useful in food and pharmaceutical materials as a cosmetic and functional.

주제어

표/그림 (3)

그림 Fig. 1. Tyrosinase (A), elastase (B), and collagenase (C) inhibitory activities of Silvetia siliquosa extract with various solvents. Values are mean±SD (n=3). The 50% inhibitory concentration (IC₅₀) values (mg/mL) were calculated from a log dose inhibition curve. Means with different letters in the same item indicate a significant difference at P<0.05 by Duncan's multiple range test. Positive controls for tyrosinase, elastase and collagenase were used kojic acid, quercetin and epigallocatechin gallate (EGCG), respectively.
그림 Fig. 2. AChE inhibitory activities of Silvetia siliquosa extract with various solvents. Values are mean±SD (n=3). The 50% inhibitory concentration (IC₅₀) values (mg/mL) were calculated from a log dose inhibition curve. Means with different letters in a column are significantly different at P<0.05 by Duncan's multiple range test. Positive controls for AChE was used tacrine which was represented in μg/mL.
그림 Fig. 3. BACE1 inhibitory activities of Silvetia siliquosa extract with various solvents. (A) EtOH and MeOH extracts. (B) Water extract. Values are mean±SD (n=3). The 50% inhibitory concentration (IC₅₀) values (μg/mL) were calculated from a log dose inhibition curve. Means with different letters in a column are significantly different at P<0.05 by Duncan's multiple range test. Positive control for BACE1 was used STA-200 (H-Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Asn-Sta-Val-Ala-Glu-Phe-OH).

AI 본문요약
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제안 방법

얻어진 각각의 추출물을 회전증발농축기(Rotavapor; RE-111; Buchi, Gwangmyeong, Korea)로 유기용매를 제거하여, 동결건조(Freeze Dryer; LP08 (XXX); Ilshinbiobase, Yangju, Korea)한 후에 -20°C 냉동고에 보관하면서 각종 실험에 사용하였다. 모든 추출물은 적절한 양을 저울에 달아 증류수로 용해하여 항산화성 및 생리활성 측정 시료로 사용하였다. 뜸부기 추출물의 수율은 각 추출물의 건량을 시료 건량으로 나누어 백분율로 나타내었다.

대상 데이터

뜸부기 분말을 3가지 용매(증류수, 70% ethanol, 80% methanol)를 사용하여 뜸부기 추출물을 각각 제조하였다. 증류수를 용매로 사용한 추출은 시료 50 g에 증류수를 시료 대비 20배의 양을 첨가하여 고압증기멸균기(autoclave; ac-11; Jeiotech, Daejon, Korea) 121°C에서 3시간 동안 가열 추출하였다.
본 연구에서 사용한 뜸부기(S. siliquosa)는 전라남도 진도에서 2021년 9월에 채취한 것을 구입하여 실험실로 운반한 후 흐르는 물로 수세하여 염분과 협잡물을 제거한 다음 자연 건조한 후 전체를 분쇄기(FM700SS; Hanil Co.

데이터처리

각 시료 간의 유의성 검정은 분산분석(ANOVA)을 한 후 P<0.05수준에서 Duncan’s multiple range test에 따라 분석하여 시료 간 유의적 차이를 검증하였다.
실험 결과는 SPSS 22.0 package program (SPSS Inc., Chicago, IL, USA)으로 통계처리 하여 3회 측정한 값의 평균±표준편차로 나타내었다

참고문헌 (23)

Ahn SM. 2012. Evaluation of biological activities of seaweeds？and development of functional food using the seaweeds.？M.S. Thesis, Andong National University, Andong, Korea.
Choi JH, Kim JS, Jo BS, Kim JH, Park HJ, An BJ, Kim MU？and Cho YJ. 2011. Biological activity in functional cosmetic？purple sweet potato extracts. Kor J Food Preserv 18, 414-422. https://doi.org/10.11002/kjfp.2011.18.3.414.

원문보기 상세보기
Cho EK and Choi YJ. 2010. Physiological activities of hot water？extracts from Ecklonia cava Kjellman. J Life Sci 20, 1675-1682. https://doi.org/10.5352/JLS.2010.20.11.1675.

원문보기 상세보기
Ellman GL, Courtney KD, Andres Jr V and Feather-stone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7, 88-90.？https://doi.org/10.1016/0006-2952(61)90145-9.

상세보기
Han YB. 2010. Edible Seaweed I-Ingredients and Bioactivity.？Korea University Press, Seoul, Korea, 247-252.
Hwang EK, Yoo HC, Ha DS and Park CS. 2015. Growth and？maturation period of Silvetia siliquosa in the natural population in Jindo, South Korea. Korean J Fish Aquat Sci 48, 745-751. https://doi.org/10.5657/KFAS.2015.0745.

원문보기 상세보기
Kim GD, Lee SY and Shin HS. 2022a. COVID-19 pandemic？and the immune regulatory function of foods. Food Sci Ind？55, 244-263. https://doi.org/10.23093/FSI.2022.55.3.244.

원문보기 상세보기
Kim HT, Han TG, Yu JH, Hwang HY, Kim HJ, Seo SJ and Kim？HK. 2022b. A comparative analysis on the relationship between health eating habits and health functional food attitudes and mood conditions between university students and？the elderly. J Convergence Cult Technol 8, 595-604. https://doi.org/10.17703/JCCT.2022.8.3.595.

원문보기 상세보기
Kim YT, Downs D, Wu S, Dashti A, Pan Y, Zhai P, Wang X,？Zhang XC and Lin X. 2002. Enzymic properties of recombinant BACE2. Eur J Biochem 269, 5668-5677. https://doi.org/10.1046/j.1432-1033.2002.03277.x.

상세보기
Kwon YR and Youn KS. 2017. Antioxidant and physiological？activities of Hijikia fusiforme by extraction methods. Korean J Food Prese 24, 631-637. https://doi.org/10.11002/kjfp.2017.24.5.631.

원문보기 상세보기
Lee SH, Lee YS, Jung SH, Shin KH and Kim BK. 2004a. Effects of the extracts from Pelvetia siliquosa on metabolism？regulation in rats. Kor J Pharmacogn 35, 203-206.
Lee SY. 2014. Analysis of skin mechanism and an application？for functional cosmetic from red bean (Phaseolus angularis)？shell extract. Ph. D. Dessertation, Hoseo University, Asan,？Korea.
Lee YJ, Jeon YJ and Kim YT. 2020. Comparison of antioxidant？and physiological activities of various solvent extracts from？Hizikia fusiformis. Korean J Fish Aquat Sci 53, 886-893.？https://doi.org/10.5657/KFAS.2020.0886.

원문보기 상세보기
Lee YJ, Kim JY, Kim WS and Kim YT. 2022. Antioxidant？and physiological activities of different solvent extracts of？Cnidium japonicum. Korean J Fish Aquat Sci 55, 310-318.？https://doi.org/10.5657/KFAS.2022.0310.

원문보기 상세보기
Lee YS, Jung SH, Lee SH and Shin KH. 2004b. Effects of the？extracts from the marine algae Pelvetia siliquosa on hyperlipidemia in tats. Kor J Pharmacogn 35, 143-146.
Lee YS, Jung SH, Lee SH, Choi YJ and Shin KH. 2002. Hepatoprotective and anti-diabetic effects of Pelvetia siliquosa, a？marine algae, in rats. J Appl Pharmacol 10, 165-169.
Lee YS, Shin KH, Kim BK and Lee SH. 2004c. Anti-diabetic？activities of fucosterol from Pelvetia siliquosa. Arch Pharm？Res 27, 1120-1122. https://doi.org/10.1007/bf02975115.

원문보기 상세보기
Na HS, Kim JY, Park JS, Choi GC, Yang SI, Lee JH, Cho JY and？Ma SJ. 2014. Characteristics of marine algae extracts using？subcritical water extract method. Korean J Food Preserv 21,？62-68. https://doi.org/10.11002/kjfp.2014.21.1.62.

원문보기 상세보기
Park DB, Lee YJ, Rho JW, Kim WS, Park SJ and Kim YT. 2022. Comparison of antioxidant and physiological activities of different solvent extracts from Codium fragile. Korean J Fish Aquat Sci 55, 858-866. https://doi.org/10.5657/KFAS.2022.0858.

원문보기 상세보기
Park WM. 2015. Anti-melanogensis and anti-wrinkle effects of？Sargassum micracanthum and Myagropsis myagroides extracts. M.S. Thesis, Pukyong University, Busan, Korea.
Seo YW and Yoo JS. 2003. Screening for antioxidizing and？tyrosinase-inhibitory activities of the extracts of marine algae from Busan coastal area. Ocean Polar Res 25, 129-132.？https://doi.org/10.4217/OPR.2003.25.1.129.

원문보기 상세보기
Son HJ, Um MY, Kim I, Cho S, Han D and Lee C. 2016. In？vitro screening for anti-dementia activities of seaweed extracts. J Korean Soc Food Sci Nutr 45, 966-972. https://doi.org/10.3746/jkfn.2016.45.7.966.

원문보기 상세보기
Talesa VN. 2001. Acetylcholinesterase in Alzheimer's disease.？Mech Ageing Dev 122, 1961-1969. https://doi.org/10.1016/S0047-6374(01)00309-8.

상세보기

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