[논문]개모시풀추출물의 Dexamethasone 유도 근위축 마우스 모델에서 근개선 효과 연구

김미선; 육흥주; 김동선; 성윤영

doi:10.6116/kjh.2023.38.5.31

[국내논문] 개모시풀추출물의 Dexamethasone 유도 근위축 마우스 모델에서 근개선 효과 연구
Effects of Boehmeria platanifolia Extract on Muscle Amelioration in Dexamethasone-Induced Muscle Atrophy Mouse Model 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.38 no.5, 2023년, pp.31 - 37

김미선 (한국한의학연구원 한의과학연구부) , 육흥주 (한국한의학연구원 한의과학연구부) , 김동선 (한국한의학연구원 한의과학연구부) , 성윤영 (한국한의학연구원 한의과학연구부)

Abstract ▼ AI-Helper

Objective : This study was conducted to investigate the muscle-improving and therapeutic effects of Boehmeria platanifolia (BP) in a mouse model of dexamethasone-induced muscle atrophy. Methods : Muscle atrophy was induced in C57BL/6 mice by intraperitoneal administration of dexamethasone for 12 days. BP extract was administered orally at doses of 100 mg/kg and 200 mg/kg for 19 days, starting 7 days before the intraperitoneal administration of dexamethasone. Mice were weighed during the experimental period, and muscle strength and muscle weight were measured at the end of the experiment. The gastrocnemius (GASTROC) muscles of mice were isolated and the cross-sectional area (CSA) of the muscle fibers was measured after H&E staining. Results : Dexamethasone-induced muscle atrophy mice had a decrease in body weight compared to normal mice, and BP-administrated mice did not show significant change in body weight compared with a control group. Muscle strength in mice with induced muscle atrophy was reduced compared to normal and significantly increased with BP administration and positive control. In addition, the weight of the quadriceps (QUAD) muscle and fiber size of the GASTROC muscle, which was reduced in sarcopenia-induced mice, was increased by BP. Conclusion : BP extract increased muscle strength, muscle weight, and muscle fiber size in dexamethasone-induced muscle atrophy mice. This suggests that the efficacy of BP extracts in improving muscle strength and preventing and treating sarcopenia may be beneficial for the development of potential therapeutic or functional products.

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표/그림 (5)

그림 Fig 1. Schematic representation of dexamethasone-induced muscle atrophy mouse model.
그림 Fig 2. Body weight change in muscle atrophy mice model. Mice were weighed 7 days prior to dexamethasone administration and on days 3 and 7. Values are means±SEM. ^#p<0.05, ^##p<0.01, ^###p<0.001 vs. Normal group.
그림 Fig 3. Effects of BP on grip strength. Strength measurements of the muscle atrophy-induced mice were performed in five replicates per mouse and expressed as the mean value. Values are means ±SEM. ^###p<0.001 vs. Normal group; *p<0.05, **P<0.01, ***p<0.001 vs. Control group.
그림 Fig 4. Effects of BP in muscle weight. Weight of GASTROC, Soleus, TA, EDL, QUAD muscle in mice with muscle atrophy. Values are means±SEM. #p<0.05 vs. Normal group; *p<0.05 vs. Control group.
그림 Fig 5. Effects of BP in muscle fiber cross-sectional area (CSA). (A) Representative H&E staining of GASTROC sections from mice with muscle atrophy induced by dexamethasone. (B) Graph of the quantified cross-sectional area of muscle fiber. Values are means SEM. ^#p<0.05 vs. Normal group; *p<0.05 vs. Control group.

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