[논문]배지에서 당근검은잎마름병균의 포자 형성에 영향을 미치는 요인

윤세나; 민지영; 김흥태

doi:10.5423/rpd.2023.29.3.251

배지에서 당근검은잎마름병균의 포자 형성에 영향을 미치는 요인
Factors Affecting Spore Formation of Carrot Leaf Blight Caused by Alternaria dauci In Vitro 원문보기

Research in plant disease = 식물병연구, v.29 no.3, 2023년, pp.251 - 257

윤세나 (충북대학교 농업생명환경대학 식물의학과) , 민지영 (충북대학교 농업생명환경대학 식물의학과) , 김흥태 (충북대학교 농업생명환경대학 식물의학과)

초록
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당근 검은잎마름병을 일으키는 Alternaria dauci의 병원성 검정을 위해서는 양과 질이 우수한 포자를 형성할 수 있는 조건의 표준화가 필요하다. 따라서 본 실험에서는 배지의 종류, 통기 처리, UV 처리 등이 A. dauci KACC42997의 포자 형성에 미치는 효과를 조사하였다. A. dauci KACC42997은 25℃, 암 상태의 PDA 배지에서 7일간 전배양하였다. 배지 상에 나타난 병원균의 공중 균사를 제거하고, 20℃에서 통기 처리와 12시간 주기의 UV 처리를 동시에 실시하며 5일간 배양하였을 때, 가장 많은 포자를 형성하였다. 확립 포자 형성 조건에서 평창, 구미, 제주 등 당근의 주요 재배지역에서 분리한 170개의 다양한 A. dauci 균주가 포자를 형성하는지의 여부를 확인한 결과, 20개 균주가 포자를 형성하지 못하였고, 88.2%의 균주는 포자를 형성하였다. 또한 지역 균주 집단 간의 포자 형성량에는 차이가 있었지만, 당근에 대한 병원성에는 차이가 없었다.

Abstract ▼ AI-Helper

In order to examine the pathogenicity of Alternaria dauci, the causal agent of carrot leaf blight, it is necessary to standardize sporulation conditions to ensure the optimal quantity and quality of spore inoculum. Therefore, in this study, the effects of the growth medium, aeration treatment, and UV treatment with 12-hr photoperiod on the sporulation of A. dauci KACC42997 were investigated. A. dauci KACC42997 was pre-cultured for 7 days in a potato dextrose agar medium at 25℃ in the dark condition. When the pre-culture, after removing aerial mycelia, was re-incubated for 5 days, with simultaneous aeration treatment and 12-hr cycle UV treatment at 20℃, the largest number of spores was produced. One hundred seventy isolates of A. dauci were isolated from major carrot growing regions such as Pyeongchang, Gumi and Jeju and tested for sporulation under the conditions established in this study. Except for 20 strains, all strains produced spores. Statistically significant differences in the extent of sporulation were found among local populations of A. dauci isolates, but no difference was observed in their pathogenicity on carrots.

주제어

표/그림 (7)

그림 Fig. 1. Disease index of Alternaria leaf blight. 0, no visible diseased lesion; 1, the case that lesions began to be observed on the leaves and the lesion area was less than 5% of the total leaf; 3, the case that typical lesions were also observed on the petiole, and the lesion area was 5% to 20% of the total leaf; 5, the case that the lesion area was 20% to 40% of the total leaf; 7, the case that the lesion area was 40% to 60% of the total leaf; 9, the case that almost all the leaves withered.
그림 Fig. 2. Spore production of Alternaria dauci KACC42997 in each medium used in the experiment. (A) Water agar medium. (B) V8 juice agar medium. (C) Potato dextrose agar medium. The A. dauci KACC42997 inoculated into each medium were cultured at 25ºC for 7 days, and then cultured at 20ºC for 5 days while aeration and UV treatment were performed simultaneously. Scale bars=200 μm.
표 Table 1. Effects of aeration, UV irradiation and media on sporulation of Alternaria dauci KACC42997
그림 Fig. 3. Pathogenicity of Alternaria dauci KACC42997 according to carrot cultivars and leaf stages. The spores of A. dauci KACC42997 formed in the potato dextrose aga medium were inoculated into 7-8 leaf stage carrots. Four kinds of carrot cultivars were used in the experiment. The spore density of the inoculum suspension was adjusted to 1.4×10⁵ conidia/ml, and after spray inoculation of the suspension, it was kept in a humidity chamber at 25ºC for 1 day and then transferred to a greenhouse. Two weeks after inoculation with the pathogen, the degree of disease was investigated using the disease index in Fig. 1.
그림 Fig. 4. Pathogenicity of Alternaria dauci KACC42997 according to spore density of spore suspension and carrot leaf stage. To produce spores, A. dauci KACC42997 was inoculated into potato dextrose agar (PDA) and cultured at 25ºC for 7 days. The pathogens were transferred to 20ºC and cultured for 5 days while aeration and UV irradiation with 12-hr cycles were performed. Spores formed on the PDA were harvested using 300 μg/ml of Tween 20 solution. The spore density of the suspension was adjusted to a defined density. The prepared spore suspension was inoculated into carrots (cv. Chamjoeun) at the 7-8 leaf stage. The disease incidence was investigated as disease index 2 weeks after inoculation.
표 Table 2. Mycelial growth and spore production of Alternaria dauci on PDA
그림 Fig. 5. Disease incidence of Alternaria dauci isolated from each region. As for the pathogens used in the experiment, 11 isolates from Pyeongchang, 6 isolates from Gumi, and 8 isolates from Jeju were selected randomly. The result of the experiment was the average disease incidence of all isolates used in each region.

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