[논문]COMPAREX 의 항염 및 진정 화장품 소재로서의 연구

김하연; 이재석; 이소헌; 김예향; 정은영; 이강혁; 신송석

doi:10.15230/scsk.2023.49.3.269

COMPAREX 의 항염 및 진정 화장품 소재로서의 연구
A Study on Anti-inflammatory and Skin Soothing Cosmetics Material of COMPAREX 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.49 no.3, 2023년, pp.269 - 276

김하연 (현대바이오랜드 생명과학연구소) , 이재석 (현대바이오랜드 생명과학연구소) , 이소헌 (현대바이오랜드 생명과학연구소) , 김예향 (현대바이오랜드 생명과학연구소) , 정은영 (현대바이오랜드 생명과학연구소) , 이강혁 (현대바이오랜드 생명과학연구소) , 신송석 (현대바이오랜드 생명과학연구소)

초록
AI-Helper

최근 다양한 외부 요인에 의해 민감해진 피부를 진정시키기 위해 더욱 안전하고 효과적인 진정 소재 개발에 대한 관심이 증가하고 있다. 따라서 본 연구에서는 항염 효능으로 잘 알려진 마치현 추출물을 기반으로 애엽, 홍경천 추출물을 혼합함으로써 COMPAREX를 제조하고, 피부 진정 효능에 미치는 영향을 마치현 단일 추출물과 비교하였다. 실험 결과, COMPAREX는 마치현 추출물 보다 LPS에 의해 유도된 NO 생성을 더욱 억제시키고, 염증성 인자인 iNOS, COX-2, TNF-α, IL-6의 발현을 현저히 억제시킴으로써 염증완화 효능이 더 효과적인 것을 확인하였다. 또한, COMPAREX는 마치현 추출물 보다 SDS 자극에 대해 IL-1α의 유전자 및 단백질 발현을 더욱 억제시킴으로써 진정 효능이 더 효과적인 것을 확인하였다. 추가적으로, 모발세포에서 COMPAREX는 PM_2.5에 의해 증가된 COX-2, IL-8의 발현을 억제시켜 두피 염증 완화 효능이 있으며, carbonylated protein을 저해시킴으로써 외부 자극으로부터 손상된 두피를 보호할 수 있는 효능을 확인하였다. 따라서, COMPAREX는 마치현 단일 추출물 보다 개선된 천연 진정 소재로의 활용 가능성을 보였고, 향후 더마 코스메틱 소재로 활용될 수 있을 것으로 기대된다.

Abstract ▼ AI-Helper

Recently, there has been a growing interest in the development of safer and more effective soothing materials to calm skin that has become sensitive to various external factors. The aim of this study was to investigate the skin soothing and anti-inflammatory effects of COMPAREX compared to Portulaca Oleracea extract. The results showed that COMPAREX significantly inhibited LPS-induced NO production and the expression of inflammatory factors iNOS, COX-2, TNF-α, and IL-6 more than P. oleracea extract. In addition, COMPAREX has been confirmed to have a more effective sedative effect by further inhibiting the gene and protein expression of IL-1α against SDS stimulation than the Portulaca Oleracea extract. Furthermore, COMPAREX inhibited the expression of inflammatory factors COX-2 and IL-8 increased by PM2.5 and suppressed H2O2-mediated carbonylated protein in hair cell. These results suggest that COMPAREX has shown the potential to be used as an improved natural soothing material over P. oleracea extract, and it is expected to be used as a derma cosmetic material in the future.

주제어

표/그림 (7)

그림 Figure 1. Cytotoxicity of COMPAREX and P. oleracea extract. Cell viability was determined by MTT assay with the concentrations of COMPAREX and P. oleracea extract for 24 h in HaCaT. The data are presented as the mean ± standard deviation from three independent experiments performed in triplicate (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the respective control group.
그림 Figure 2. Cytotoxicity of COMPAREX. Cell viability was determined by MTT assay with the concentrations of COMPAREX for 24 h in (A) HHGMC and (B) HHORSC. The data are presented as the mean ± standard deviation from three independent experiments performed in triplicate ( (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the respective control group.
그림 Figure 3. Cytotoxicity and effect of COMPAREX and three single extracts on the NO production. Cell viability and nitric oxide (NO) level were determined by MTT assay and griess reagent with the concentrations of COMPAREX and three single extracts for 24 h in Raw264.7. The data are presented as the mean ± standard deviation from three independent experiments performed in triplicate (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the respective LPS only treated group.
그림 Figure 4. Effect of COMPAREX and P. oleracea extract on anti-inflammation. The mRNA expression of iNOS, COX-2, TNF-α and IL-6 were determined by real-time PCR. β-actin was used as a house keeping gene. The data are presented as the mean ± standard deviation from three independent experiments performed in triplicate (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the respective LPS only treated group.
그림 Figure 5. E ffect o f COMPAREX a nd P. oleracea extract on skin soothing. (A) The mRNA expression of IL-1α was determined by real-time PCR. β-actin was used as a house keeping gene. (B) The protein expression of IL-1α was determined by ELISA. The data are presented as the mean ± standard deviation from three independent experiments performed in triplicate (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the respective SDS only treated group.
그림 Figure 6. Effect of COMPAREX on PM-induced hair loss. The mRNA expression of COX-2, IL-8 were determined by real-time PCR. β-actin was used as a house keeping gene. The data are presented as the mean ±standard deviation from three independent experiments performed in triplicate (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the respective PM 2.5 only treated group.
그림 Figure 7. Effect of CO MPAREX on H₂O₂-mediated protein carbonylation. HHORSCs were stained with FTSC and hoechst 33342. Intracellular CPs were visualized by fluorescence labelling with FTSC. (A) Their morphology and fluorescence intensity were observed with a fluorescence microscope. (B) CP levels were analysed by ELISA reader. The data are presented as the mean ± standard deviation from three independent experiments performed in triplicate (N = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. the respective H₂O₂ only treated group.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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