[논문]NF-κB 활성 조절을 통한 Lactobacillus johnsonii 파쇄액의 항염 효과

차화준

doi:10.15230/scsk.2023.49.3.285

NF-κB 활성 조절을 통한 Lactobacillus johnsonii 파쇄액의 항염 효과
Anti-inflammatory Effects of Lactobacillus johnsonii Lysate via Regulation of NF-κB Activity 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.49 no.3, 2023년, pp.285 - 290

차화준 (오산대학교, 뷰티코스메틱계열)

초록
AI-Helper

본 연구에서는 김치유래의 Lactobacillus johnsonii의 anti-inflammation 효능을 확인하였다. Rat 유래 대식세포인 Raw 264.7세포에 Lactobacillus johnsonii 파쇄물을 처리하여 염증유발 marker인 TNFα와 IL1β의 발현량을 확인한 결과 250 ㎍/mL 추출물을 처리시 1 ㎍/mL LPS를 처리한 대조군 대비 각각 40.55%와 34.66%의 TNFα와 IL1β의 발현량 감소를 확인하였다. 또한 LPS에 의한 cytokine 발현의 핵심 전사인자인 NF-κB의 전사활성을 확인한결과 1 ㎍/mL LPS를 처리한 대조군 대비 NF-κB의 전사활성은 40.76% 억제됨을 확인하였다. 때문에 본 연구결과를 통해 Lactobacillus johnsonii 파쇄물은 LPS에 의한 cytokine의 발현을 방지 및 NF-κB전사활성 조절을 통해 항염 또는 피부진정 기능성 소재로써 가능성을 확인하였다.

Abstract ▼ AI-Helper

In this study, the anti-inflammation efficacy of Lactobacillus johnsonii derived from Kimchi was investigated. Raw 264.7 cells, which are rat-derived macrophages, were treated with Lactobacillus johnsonii lysate to confirm the expression level of TNFα and IL1β, which are inflammatory markers, and when treating 250 ㎍/mL extract, the expression level of TNFα and IL1β decreased by 40.55% and 34.66% compared to the control group treated with 1 ㎍/mL LP, respectively. In addition, as a result of confirming the transcriptional activity of NF-κB, a key transcription factor in cytokine expression by LPS, it was confirmed that the transcriptional activity of NF-κB was 40.76% inhibited compared to the control group treated with 1 ㎍/mL LPS. Therefore, the results of this study confirmed that Lactobacillus johnsonii lysate is likely to be an anti-inflammatory or skin-soothing functional material by preventing the expression of cytokine by LPS and controlling NF-κB transcriptional activity.

주제어

표/그림 (4)

그림 Figure 1. The cytotoxicity of Lactobacillus johnsonii lysate. Raw264.7 cells were treated with Lactobacillus johnsonii lysate (concentration is μg/mL) for 48 h, 37 ℃. The result is presented as the mean ± SD of the experiment (N = 3). *p < 0.05 (vs. control).
그림 Figure 2. The effect of Lactobacillus johnsonii lysate on TNFα and IL1β mRNA. Raw264.7 cells were treated with LPS and Lactobacillus johnsonii lysate (concentration is μg/mL) for 24 h, 37 ℃. The result is presented as the mean ± SD of t he e xperiment (N = 3 ). ^#p < 0.05 (vs. control), *p < 0.05 (vs. LPS treated Raw264.7 cells).
그림 Figure 3. The effect of Lactobacillus johnsonii lysate on TNFα and IL1β. Raw264.7 cells were treated with LPS and Lactobacillus johnsonii lysate (concentration is μg/mL) for 24 h, 37 ℃. The result is presented as the mean ± SD of the experiment (N = 3). ^#p < 0.05 (vs. control), *p < 0.05 (vs. LPS treated Raw264.7 cells).
그림 Figure 4. The effect of Lactobacillus johnsonii lysate on NF-κB transcription activity. Raw264.7 cells were treated with LPS and Lactobacillus johnsonii lysate (concentration is μg/mL) for 24 h, 37 ℃. The result is presented as the mean ± SD of the experiment (N = 3). ^#p < 0.05 (vs. control), *p < 0.05 (vs. LPS treated Raw264.7 cells).

참고문헌 (25)

M. Higuchi, N. Higashi, H. Taki, and T. Osawa, Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages, J Immunol., 144(4), 1425 (1990).？

상세보기
E. Park, H. I. Rhee, H. Jung, S. M. Ju, Y. Lee, S. Lee, S. Hong, H. Yang, M. Yoo, and K. S. Kim, Anti-inflammatory effects of a combined herbal preparation (RAH13) of Phellodendron amurense and Coptis chinensis in animal models of inflammation, Phytother Res., 21(8), 746 (2007).？
N. R. Ferreri, I. Millet, V. Paliwal, W. Herzog, D. Solomon, R. Ramabhadran, and P. W. Askenase, Induction of macrophage TNF alpha, IL-1, IL-6, and PGE2 production by DTH-initiating factors, Cell Immunol., 137(2), 389 (1991).？
T. Liu, L. Zhang, D. Joo, and S. Sun, NF-κB signaling in inflammation, Signal Transduct Target Ther., 2(1), 17023 (2017).？
H. Zhang and S. Sun, N F-κB in inflammation and renal diseases, Cell Biosci., 5(1), 63 (2015).？
T. Lawrence, The nuclear factor NF-κB pathway in inflammation, Cold Spring Harb Perspect Biol., 1(6), a001651 (2009).？
D. Sosic, J. A. Richardson, K. Yu, D. M. Ornitz, and E. N. Olson, Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity, Cell, 112(2), 169 (2003).？
I. Sur, M. Ulvmar, and R. Toftgard, The two-faced NF-kappaB in the skin, Int Rev Immunol., 27(4), 205 (2008).？
S. Bell, K. Degitz, M. Quirling, N. Jilg, S. Page, and K. Brand, Involvement of NF-kappaB signalling in skin physiology and disease, Cell Signal., 15(1), 1 (2003).？
A. Tanaka, S. Muto, K. Jung, A. Itai, and H. Matsuda, Topical application with a new NF-kappaB inhibitor improves atopic dermatitis in NC/NgaTnd mice, J. Invest. Dermatol., 127(4), 855 (2007).？
J. Kim and K. W. Lee, Molecular targets of phytochemicals for skin inflammation, Curr Pharm Des., 24(14), 1533 (2018).？

상세보기
S. A. Shin, B. J. Joo, J. S. Lee, G. Ryu, M. Han, W. Y. Kim, H. H. Park, J. H. Lee, and C. S. Lee, Phytochemicals as anti-inflammatory agents in animal models of prevalent inflammatory diseases, Molecules, 25(24), 5932 (2020).？

상세보기
S. Wu, Y. Pang, Y. He, X. Zhang, L. Peng, J. Guo, and J. Zeng, A comprehensive review of natural products against atopic dermatitis: Flavonoids, alkaloids, terpenes, glycosides and other compounds, Biomed Pharmacother., 140(1), 111741 (2021).？
M. Sun, Y. Deng, X. Cao, L. Xiao, Q. Ding, F. Luo, P. Huang, Y. Gao, M. Liu, and H. Zhao, Effects of natural polyphenols on skin and hair health: A review, Molecules, 27(22), 7832 (2022).？

상세보기
M. Dzialo, J. Mierziak, U. Korzun, M. Preisner, J. Szopa, and A. Kulma, The potential of plant phenolics in prevention and therapy of skin disorders, Int. J. Mol. Sci., 17(2), 160 (2016).？
R. Ayyanna, D. Ankaiah, and V. Arul, Anti-inflammatory and antioxidant properties of probiotic bacterium Lactobacillus mucosae AN 1 and Lactobacillus fermentum SN R1 in wistar albino rats, Front Microbiol., 9(1), 3063 (2018).？
K. J. Han, J. Lee, N. Lee, and H. Paik, Antioxidant and anti-Inflammatory effect of probiotic Lactobacillus plantarum KU15149 derived from Korean homemade diced-radish Kimchi, J. Microbiol. Biotechnol., 30(4), 591 (2020).？

원문보기 상세보기
Y. Cai, F. Xue, C. Quan, M. Qu, N . Liu, Y. Zhang, C. Fleming, X. Hu, H. Zhang, R. Weichselbaum, Y. Fu, D. Tieri, E. C. Rouchka, J. Zheng, and J. Yan, A critical role of the IL-1β-IL-1R signaling pathway in skin inflammation and psoriasis pathogenesis, J. Invest. Dermatol., 139(1), 146 (2019).？
L. Calabrese, Z. Fiocco, T. K. Satoh, K. Peris, and L. E. French, Therapeutic potential of targeting interleukin-1 family cytokines in chronic inflammatory skin diseases, Br. J. Dermatol., 186(6), 925 (2022).？

상세보기
M. M. Bashir, M. R. Sharma, and V. P. Werth, TNF-alpha production in the skin, Arch. Dermatol. Res., 301(1), 87 (2009).？
R. W. Groves, M. H. Allen, E. L. Ross, J. N. Barker, and D. M. MacDonald, Tumour necrosis factor alpha is pro-inflammatory in normal human skin and modulates cutaneous adhesion molecule expression, Br. J. Dermatol., 132(3), 345 (1995).？

상세보기
A. V. Kostarnoy, P. G. Gancheva, D. Y. Logunov, L. V. Verkhovskaya, M. A. Bobrov, D. V. Scheblyakov, A. I. Tukhvatulin, N. E. Filippova, B. S. Naroditsky, and A. L. Gintsburg, Topical bacterial lipopolysaccharide application affects inflammatory response and promotes wound healing, J Interferon Cytokine Res., 33(9), 514 (2013).？

상세보기
A. Xie, A. Chen, Y. Chen, Z. Luo, S. Jiang, D. Chen, and R. Yu, Lactobacillus for the treatment and prevention of atopic dermatitis: Clinical and experimental evidence, Front Cell Infect Microbiol, 13(1), 1137275 (2023).？
A. Thoma, and A. P. Lightfoot, NF-kB and inflammatory cytokine signalling: Role in skeletal muscle atrophy, Adv Exp Med Biol, 1088(1), 267 (2018).？
J. P. Cogswell, M. M. Godlevski, G. B. Wisely, W. C. Clay, L. M. Leesnitzer, J. P. Ways, and J. G. Gray, NF-kappa B regulates IL-1 beta transcription through a consensus NF-kappa B binding site and a nonconsensus CRE-like site, J. Immunol., 153(2), 712 (1994).

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증