[논문]Brachythecium populeum 추출물의 항산화 및 항염효과

박상남; 이옥희

doi:10.15324/kjcls.2023.55.3.174

Brachythecium populeum 추출물의 항산화 및 항염효과
Antioxidant and Anti-Inflammatory Activity of Brachythecium populeum Extract 원문보기

Korean journal of clinical laboratory science : KJCLS = 대한임상검사과학회지, v.55 no.3, 2023년, pp.174 - 183

박상남 (경동대학교 임상병리학과) , 이옥희 (경동대학교 보건관리학과)

초록
AI-Helper

Brachythecium populeum 의 상업적 사용 가능성을 확인하기 위하여 항산화능 실험과 세포 독성, 항염능 실험을 실시하였다. 폴리페놀과 플라보노이드 농도 측정에서는 70% 에탄올 추출이 증류수 추출에 비해 높은 농도를 나타내어, 70% 에탄올이 추출물의 유효성분 및 추출 수율의 측면에서 가장 적합한 추출용매란 것을 확인하였다. DPPH, ABTS에서는 증류수 추출물에 비해 70% 에탄올 추출물의 항산화능이 높은 것을 확인하였다. 이는 B. populeum 유효성분의 극성에 따른 것으로 생각된다. 항염능 실험에서는 세포 독성과 항염능을 알아보았다. 세포독성의 경우 양 추출물 모두낮은 세포독성을 보였으며, 염증 전달 물질인 NO 생성 억제능의 경우 70% 에탄올 추출물이 증류수 추출물에 비해 통계적으로 유의한 수준의 항염능 증가를 보였다. 염증성 사이토카인인 IL-1β, IL-6, TNF-α의 농도를 측정한 결과 100 ㎍/mL 농도에서 각각 9.39%, 11.87%, 14.49%의 억제효과를 나타내었다. 이를 통해 B. populeum의 70% 에탄올이 염증 억제를 위한 물질로서 사용 가능함을 보였다.

Abstract ▼ AI-Helper

Antioxidant, cytotoxic, and anti-inflammatory assays were conducted to determine the commercial viability of Brachythecium populeum. The antioxidant activity was assessed by performing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. This was followed by the quantification of polyphenols and flavonoids. Results of the DPPH and ABTS assay showed that antioxidant activities of the ethanol extract of B. populeum were 3.7 and 3.6 times higher than water extract, respectively. The polyphenol concentration was also determined to be 4.1 times higher and the flavonoid concentration was 5.3 times higher than the water extract. The cell-based experiments, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and nitric oxide assay, were performed using RAW 264.7. Results of the MTT assay revealed that both extracts exerted no cytotoxicity on the cells (based on 80% viability). In the nitric oxide (NO) production inhibition experiment, inhibition of NO production was determined to be 15.42% more when exposed to ethanol extract as compared to water extract. Furthermore, the ethanol extract exerted greater inhibition of inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor-α production (9.39%, 11.87%, and 14.49% more, respectively) when compared to the water extract. Due to the good antioxidant activity and potential for inhibiting NO and inflammatory cytokine production, B. populeum ethanol extracts are prospective sources of anti-inflammatory compounds.

주제어

표/그림 (10)

그림 Figure 1. Total phenolic contents of Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum; GAE, gallic acid equivalents; DW, distilled water. ***P<0.001.
그림 Figure 2. Total flavonoid contents of Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum; QE, quercetin equivalents; DW, distilled water. ***P<0.001.
표 Table 1. Dry weight and yield of Brachythecium populeum extract
그림 Figure 3. DPPH radical scavenging rate of Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum; DPPH, 2,2-Diphenyl-1-picrylhydrazyl; Cont., control group. **P<0.01, ***P<0.001.
그림 Figure 4. ABTS radical scavenging rate of Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum ; ABTS, 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid); Cont., control group. **P <0.01, ***P<0.001.
그림 Figure 5. Cell survival rate of RAW 264.7 with Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum.
그림 Figure 6. Nitric oxide production rate of RAW 264.7 with Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum; Cont. -, control group without lipopolysaccharide (LPS); Cont. +, control group with LPS. Compared with water extracts; *P<0.05, **P<0.01.
그림 Figure 7. IL-1β production rate of RAW 264.7 with Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum; IL-1β, interleukin-1β; Cont. -, control group without LPS; Cont. +, control group with lipopolysaccharide (LPS). Compared with water extracts; *P<0.05.
그림 Figure 8. IL-6 production rate of RAW 264.7 with Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum; IL-6, interleukin-6; Cont. -, control group without lipopolysaccharide (LPS); Cont. +, control group with LPS. Compared with water extracts; *P<0.05, **P<0.01.
그림 Figure 9. TNF-α production rate of RAW 264.7 with Brachythecium populeum extract. Abbreviations: WE, water extract of B. populeum; EE, 70% ethanol extract of B. populeum. Compared with water extracts; *P<0.05, **P<0.01, ***P<0.001.

참고문헌 (41)

Kim TN. Elderly obesity: is it harmful or beneficial? J Obes Metab？Syndr. 2018;27:84-92. https://doi.org/10.7570/jomes.2018.27.2.84

상세보기
Ko MS. A study on research trends of age-friendly using text network analysis: focusing on 「The Korean Journal of Health Service？Management」 (2007-2018). Korean Soc Health Serv Manag.？2019;13:19-31. https://doi.org/10.12811/kshsm.2019.13.4.019

원문보기 상세보기
Di Micco R, Krizhanovsky V, Baker D, d'Adda di Fagagna F.？Cellular senescence in ageing: from mechanisms to therapeutic？opportunities. Nat Rev Mol Cell Biol. 2021;22:75-95. https://doi.org/10.1038/s41580-020-00314-w

상세보기
Scudellari M. To stay young, kill zombie cells. Nature. 2017;550:448-450. https://doi.org/10.1038/550448a

상세보기
von Kobbe C. Targeting senescent cells: approaches, opportunities, challenges. Aging (Albany NY). 2019;11:12844-12861.？https://doi.org/10.18632/aging.102557

상세보기
Pole A, Dimri M, Dimri GP. Oxidative stress, cellular senescence？and ageing. AIMS Mol Sci. 2016;3:300-324. https://doi.org/10.3934/molsci.2016.3.300
Davalli P, Mitic T, Caporali A, Lauriola A, D'Arca D. ROS, cell？senescence, and novel molecular mechanisms in aging and？age-related diseases. Oxid Med Cell Longev. 2016;2016:3565127.？https://doi.org/10.1155/2016/3565127

상세보기
Sies H. Strategies of antioxidant defense. Eur J Biochem. 1993;？215:213-219. https://doi.org/10.1111/j.1432-1033.1993.tb18025.x

상세보기
Berneburg M, Plettenberg H, Krutmann J. Photoaging of human？skin. Photodermatol Photoimmunol Photomed. 2000;16:239-244.？https://doi.org/10.1034/j.1600-0781.2000.160601.x

상세보기
Pilkington SM, Bulfone-Paus S, Griffiths CEM, Watson REB.？Inflammaging and the skin. J Invest Dermatol. 2021;141(4S):1087-1095. https://doi.org/10.1016/j.jid.2020.11.006

상세보기
Seo JY, Chung JH. Thermal aging: a new concept of skin aging. J？Dermatol Sci Suppl. 2006;2:S13-S22. https://doi.org/10.1016/j.descs.2006.08.002

상세보기
Franceschi C, Garagnani P, Parini P, Giuliani C, Santoro A.？Inflammaging: a new immune-metabolic viewpoint for age-related diseases. Nat Rev Endocrinol. 2018;14:576-590. https://doi.org/10.1038/s41574-018-0059-4

상세보기
Minciullo PL, Catalano A, Mandraffino G, Casciaro M, Crucitti A,？Maltese G, et al. Inflammaging and anti-inflammaging: the role of？cytokines in extreme longevity. Arch Immunol Ther Exp (Warsz).？2016;64:111-126. https://doi.org/10.1007/s00005-015-0377-3

상세보기
Herwald H, Egesten A. Tackling the pros and cons of inflammation.？J Innate Immun. 2019;11:445-446. https://doi.org/10.1159/000502353

상세보기
Chovatiya R, Medzhitov R. Stress, inflammation, and defense of？homeostasis. Mol Cell. 2014;54:281-288. https://doi.org/10.1016/j.molcel.2014.03.030

상세보기
Checa J, Aran JM. Reactive oxygen species: drivers of physiological？and pathological processes. J Inflamm Res. 2020;13:1057-1073.？https://doi.org/10.2147/jir.s275595

상세보기
Nathan C, Ding A. Nonresolving inflammation. Cell. 2010;140:871-882. https://doi.org/10.1016/j.cell.2010.02.029

상세보기
Evans JA, Johnson EJ. The role of phytonutrients in skin health.？Nutrients. 2010;2:903-928. https://doi.org/10.3390/nu2080903

상세보기
Yim EY, Choi HS. Assessment on the biological activities of ethanol extract from Marchantia polymorpha L. Korean J Med Crop？Sci. 2021;29:187-193. https://doi.org/10.7783/KJMCS.2021.29.3.187

상세보기
National Institute of Biological Resources Biodiversity on the？Korean Peninsula [Internet]. National Institute of Biological？Resources [cited 2023 November 7]. Available from: https://species.nibr.go.kr/home/mainHome.do?contCd009002&ktsn120000054214
Bum HM, Park SJ, Bakalin VA, Choi B, Sim SH, Hyun CW, et al.？Bryophyte flora of Taebaeksan Mountain National Park in Korea.？Korean J Plant Taxon. 2020;50:262-278. https://doi.org/10.11110/kjpt.2020.50.3.262

원문보기 상세보기
Angalao LA, Doctor JGP, Banwa T. Antimicrobial activities of？Azolla filiculoides Lam. (Pteridophyte) and Brachythecium buchananii (Hook.) Jaeg. (Bryophyte). Int J Sci Clin Lab. 2012;2:71-81.
Greeshma GM, Manoj GS, Murugan K. Insight into pharmaceutical importance of bryophytes. Kong Res J. 2017;4:84-88.
Singh M, Rawat AK, Govindarajan R. Antimicrobial activity of？some Indian mosses. Fitoterapia. 2007;78:156-158. https://doi.org/10.1016/j.fitote.2006.10.008

상세보기
Larrauri JA, Ruperez P, Saura-Calixto F. Effect of drying temperature on the stability of polyphenols and antioxidant activity of？red grape pomace peels. J Agric Food Chem. 1997;45:1390-1393.？https://doi.org/10.1021/jf960282f

상세보기
Antony A, Farid M. Effect of temperatures on polyphenols during？extraction. Appl Sci. 2022;12:2107. https://doi.org/10.3390/app12042107

상세보기
Aboud SA, Altemimi AB, R S Al-HiIphy A, Yi-Chen L, Cacciola F.？A comprehensive review on infrared heating applications in food？processing. Molecules. 2019;24:4125. https://doi.org/10.3390/molecules24224125

상세보기
Leal PF, Maia NB, Carmello QAC, Catharino RR, Eberlin MN,？Meireles MAA. Sweet basil (Ocimum basilicum) extracts obtained？by supercritical fluid extraction (SFE): global yields, chemical？composition, antioxidant activity, and estimation of the cost of？manufacturing. Food Bioprocess Technol. 2008;1:326-338.？https://doi.org/10.1007/s11947-007-0030-1

상세보기
Pekal A, Pyrzynska K. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal Methods. 2014;7:？1776-1782. https://doi.org/10.1007/s12161-014-9814-x

상세보기
Blois MS. Antioxidant determinations by the use of a stable free？radical. Nature. 1958;181:1199-1200. https://doi.org/10.1038/1811199a0

상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans？C. Antioxidant activity applying an improved ABTS radical cation？decolorization assay. Free Radic Biol Med. 1999;26:1231-1237.？https://doi.org/10.1016/s0891-5849(98)00315-3

상세보기
Chen Z, Bertin R, Froldi G. EC50 estimation of antioxidant activity in DPPH.assay using several statistical programs. Food Chem. 2013;138:414-420. https://doi.org/10.1016/j.foodchem.2012.11.001

상세보기
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo？FE, Ismadji S, et al. Effect of extraction solvent on total phenol？content, total flavonoid content, and antioxidant activity of？Limnophila aromatica. J Food Drug Anal. 2014;22:296-302.？https://doi.org/10.1016/j.jfda.2013.11.001

상세보기
Stalikas CD. Extraction, separation, and detection methods for？phenolic acids and flavonoids. J Sep Sci. 2007;30:3268-3295.？https://doi.org/10.1002/jssc.200700261

상세보기
Zielinski H, Kozlowska H. Antioxidant activity and total phenolics in selected cereal grains and their different morphological？fractions. J Agric Food Chem. 2000;48:2008-2016. https://doi.org/10.1021/jf990619o

상세보기
Smolinska-Kondla D, Zych M, Ramos P, Waclawek S, Stebel A.？Antioxidant potential of various extracts from 5 common European？mosses and its correlation with phenolic compounds. Herba Pol.？2022;68:54-68. https://doi.org/10.2478/hepo-2022-0014

상세보기
Sabovljevic MS, Sabovljevic AD, Ikram NKK, Peramuna A, Bae H,？Simonsen HT. Bryophytes - an emerging source for herbal remedies？and chemical production. Plant Genet Resour. 2016;14:314-327.？https://doi.org/10.1017/S1479262116000320

상세보기
Singh A, Yau YF, Leung KS, El-Nezami H, Lee JC. Interaction of？polyphenols as antioxidant and anti-inflammatory compounds in？brain-liver-gut axis. Antioxidants (Basel). 2020;9:669. https://doi.org/10.3390/antiox9080669

상세보기
Yang CY, Pan CC, Tseng CH, Yen FL. Antioxidant, anti-inflammation and antiaging activities of Artocarpus altilis methanolic extract on urban particulate matter-induced HaCaT keratinocytes damage. Antioxidants (Basel). 2022;11:2304. https://doi.org/10.3390/antiox11112304

상세보기
Kim SY, Hong M, Kim TH, Lee KY, Park SJ, Hong SH, et al.？Anti-inflammatory effect of liverwort (Marchantia polymorpha？L.) and Racomitrium moss (Racomitrium canescens (Hedw.) Brid.)？growing in Korea. Plants (Basel). 2021;10:2075. https://doi.org/10.3390/plants10102075

상세보기
Marques RV, Sestito SE, Bourgaud F, Miguel S, Cailotto F, Reboul？P, et al. Anti-inflammatory activity of bryophytes extracts in？LPS-stimulated RAW264.7 murine macrophages. Molecules.？2022;27:1940. https://doi.org/10.3390/molecules27061940

상세보기

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동의어: Packet Collision Rate

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