[논문]꾸지뽕나무 잎과 우슬 복합물이 MIA로 골관절염이 유도된 Rat에 미치는 영향

정길호; 주인환; 김동희

doi:10.15188/kjopp.2023.06.37.3.56

꾸지뽕나무 잎과 우슬 복합물이 MIA로 골관절염이 유도된 Rat에 미치는 영향
Effect of Cudrania tricuspidata Leaf and Achyranthes japonica Nakai Complex in MIA-Induced Osteoarthritis Rats 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.37 no.3, 2023년, pp.56 - 60

정길호 (대전대학교 한의과대학 병리학교실) , 주인환 (대전대학교 한의과대학 병리학교실) , 김동희 (대전대학교 한의과대학 병리학교실)

Abstract ▼ AI-Helper

This study was to investigate the effects of cudrania tricuspidata leaf and achyranthes japonica nakai complex (CAC) treatment on monosodium iodoacetate (MIA)-induced osteoarthritis rats. Osteoarthritis was induced by injection of MIA (3 mg) into right knee joints of rats. The rats were divided into 5 groups; Non-induced normal rat (Con, n=10), MIA-induced osteoarthritis rat (MIA, n=10), osteoarthritis rat treated with indomethacin 2 mg/kg (Indo, n=10), osteoarthritis rat treated with CAC 200 mg/kg (Low, n=10) or 400 mg/kg (High, n=10). The rats were treated orally for 14 days. On the last day of oral administration, the hind paw weight bearing of the experimental animals was measured using an incapacitance test meter. The interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2), cartilage oligomeric matrix protein (COMP) and, C-telopeptides of type II collagen (CTX-II) levels in serum were measured by ELISA. And Then, that gene expression (IL-1β, IL-6, TNF-α, COX-2) levels in cartilage were measured by qRT-PCR. Also, cartilage volume was measured by micro CT arthrography. Administration of CAC reduced the inflammatory cytokine (IL-1β, IL-6, TNF-α, PGE2) levels in serum and gene expression levels in cartilage, and the COMP and CTX-II level in serum, which is an indicator of cartilage degradation. In addition, the hind paw weight bearing and cartilage volume, which were decreased due to osteoarthritis, were significantly increased. In the future, if the results of clinical studies including studies on safety are supplemented, it is considered that it can be used as an oriental medicine treatment to improve or treat symptoms of osteoarthritis.

주제어

표/그림 (6)

그림 Fig. 1. Effects of CAC on hind paw weight bearing of MIA-induced osteoarthritis rat. The result were presented by the mean±standard error of mean (N=10). Significance of results, +++ : p<0.001 compared to Con, ** ; p<0.01, *** ; p<0.001 compared to MIA. Con; MIA-untreated group, MIA; MIA-induced osteoarthritis rat, Indo; MIA-induced osteoarthritis rat treated with indomethacin (2 mg/kg), Low or High; MIA-induced osteoarthritis rat treated with CAC (200 or 400 mg/kg).
표 Table 1. Real-Time PCR Primer Sequences
그림 Fig. 2. Effects of CAC on cytokine level in serum of MIA-induced osteoarthritis rat. The result were presented by the mean±standard error of mean (N=10). (A) IL-1β, (B) IL-6, (C) TNF-α, (D) PGE2 level in serum. Significance of results, +++ : p<0.001 compared to Con, *** ; p<0.001 compared to MIA. Groups are the same as in Fig. 1.
그림 Fig. 3. Effects of CAC on gene expression level in cartilage of MIA-induced osteoarthritis rat. The result were presented by the mean±standard error of mean (N=10). (A) IL-1β, (B) IL-6, (C) TNF-α, (D) COX-2 expression level in cartilage. Significance of results, +++ : p<0.001 compared to Con, * ; p<0.05, ** ; p<0.01, *** ; p<0.001 compared to MIA. Groups are the same as in Fig. 1.
그림 Fig. 4. Effects of CAC on COMP and CTX-2 level in serum of MIA-induced osteoarthritis rat. The result were presented by the mean±standard error of mean(N=10). (A) COMP, (B) CTX-2 level in serum. Significance of results, +++ : p<0.001 compared to Con, ** ; p<0.01, *** ; p<0.001 compared to MIA. Groups are the same as in Fig. 1.
그림 Fig. 5. Effects of CAC on cartilage volume in MIA-induced osteoarthritis rat. The result were presented by the mean±standard error of mean(N=10). (A) Micro-CT results, (B) Cartilage volume. Significance of results, +++ : p<0.001 compared to Con, ** ; p<0.01 compared to MIA. Groups are the same as in Fig. 1.

참고문헌 (37)

Kraus VB, Blanco FJ, Englund M, Karsdal MA,？Lohmander LS. Call for standardized definitions of？osteoarthritis and risk stratification for clinical trials？and clinical use. Osteoarthritis and cartilage.？2015;23(8):1233-41.
Chow YY, Chin K-Y. The role of inflammation in the？pathogenesis of osteoarthritis. Mediators of？inflammation. 2020;2020.

상세보기
Yorifuji M, Sawaji Y, Endo K, Kosaka T, Yamamoto K.？Limited efficacy of COX-2 inhibitors on nerve growth？factor and metalloproteinases expressions in human？synovial fibroblasts. Journal of Orthopaedic Science.？2016;21(3):381-8.
Castaneda S, Roman-Blas JA, Largo R,？Herrero-Beaumont G. Subchondral bone as a key target？for osteoarthritis treatment. Biochemical pharmacology.？2012;83(3):315-23.

상세보기
Olszewska-Slonina DM, Matewski D, Drewa G, Wozniak？A, Czajkowski R, Rajewski P, et al. Oxidative equilibrium？in the prophylaxis of degenerative joint changes: an？analysis of pre-and postoperative activity of antioxidant？enzymes in patients with hip and knee osteoarthritis.？Med Sci Monit. 2010;16(5):238-45.
Ou Y, Tan C, An H, Jiang D, Quan Z, Tang K, et al.？Selective COX-2 inhibitor ameliorates osteoarthritis by？repressing apoptosis of chondrocyte. Medical Science？Monitor: International Medical Journal of Experimental？and Clinical Research. 2012;18(6):BR247.

상세보기
Pecchi E, Priam S, Mladenovic Z, Gosset M, Saurel A-S,？Aguilar L, et al. A potential role of chondroitin sulfate？on bone in osteoarthritis: inhibition of prostaglandin E2？and matrix metalloproteinases synthesis in interleukin-1？β-stimulated osteoblasts. Osteoarthritis and cartilage.？2012;20(2):127-35.
Chang SH, Jung EJ, Lim DG, Oyungerel B, Lim KI, Her？E, et al. Anti-inflammatory action of Cudrania？tricuspidata on spleen cell and T lymphocyte？proliferation. Journal of Pharmacy and Pharmacology.？2008;60(9):1221-6.
Kim D-W, Lee W-J, Asmelash Gebru Y, Choi H-S, Yeo？S-H, Jeong Y-J, et al. Comparison of bioactive？compounds and antioxidant activities of Maclura？tricuspidata fruit extracts at different maturity stages.？Molecules. 2019;24(3):567.

상세보기
Han XH, Hong SS, Jin Q, Li D, Kim H-K, Lee J, et al.？Prenylated and benzylated flavonoids from the fruits of？Cudrania tricuspidata. Journal of natural products.？2009;72(1):164-7.
Lee H, Ha H, Lee JK, Seo Cs, Lee Nh, Jung DY, et al.？The fruits of Cudrania tricuspidata suppress？development of atopic dermatitis in NC/Nga mice.？Phytotherapy Research. 2012;26(4):594-9.

상세보기
Lee YJ, Kim S, Lee SJ, Ham I, Whang WK. Antioxidant？activities of new flavonoids from Cudrania tricuspidata？root bark. Archives of pharmacal research.？2009;32:195-200.
Jeong G-S, Lee D-S, Kim Y-C. Cudratricusxanthone A from Cudrania tricuspidata suppresses？pro-inflammatory mediators through expression of？anti-inflammatory heme oxygenase-1 in RAW264. 7？macrophages. International Immunopharmacology.？2009;9(2):241-6.
Song S-H, Ki SH, Park D-H, Moon H-S, Lee C-D, Yoon？I-S, et al. Quantitative analysis, extraction optimization,？and biological evaluation of Cudrania tricuspidata leaf？and fruit extracts. Molecules. 2017;22(9):1489.

상세보기
Kim OK, Lee J. Therapeutic effects of Curdrania？tricuspidata leaf extract on osteoarthritis. Journal of the？Korean Society of Food Science and Nutrition.？2013;42(5):697-704.
Al-Mijan M, Park H, Lee Y, Lim B. Evaluation of the？antioxidant and anti-inflammatory potential of？fermented Achyranthes japonica Nakai extract. Nat？Prod Chem Res. 2018;6(5):337-43.
Park J, Kim I. Effects of dietary Achyranthes japonica？extract supplementation on the growth performance,？total tract digestibility, cecal microflora, excreta noxious？gas emission, and meat quality of broiler chickens.？Poultry science. 2020;99(1):463-70.

상세보기
Iqbal Z, Shah Y, Ahmad L. Evaluation of？anti-inflammatory activity of selected medicinal plants？of Khyber Pakhtunkhwa, Pakistan. Pak J Pharm Sci.？2014;27(2):365-8.
Jang G-Y, Kim H-Y, Lee S-H, Kang Y-R, Hwang I-G,？Woo K-S, et al. Effects of heat treatment and？extraction method on antioxidant activity of several？medicinal plants. Journal of the Korean society of food？science and nutrition. 2012;41(7):914-20.
Kim C-S, Park Y-K. The therapeutic effect of？Achyranthis Radix on the collagen-induced arthritis in？mice. The Korea Journal of Herbology.？2010;25(4):129-35.
Kim K-Y, Kim S-W, Kim J-K, Ko S-Y. Effects of？achyranthes radix extracts on osteoblasts and？osteocalsts. International Journal of Oral Biology.？2005;30(2):39-45.

원문보기 상세보기
Jang SH, Lee SH, Lee HS, Seo Y, Song H-J, Hwang HS,？et al. The effects of fermented Achyranthes japonica？Radix extract on osteoarthritis model in rats. Korean？Association for Laboratory Animal Science Symposium.？2015:150.
HUANG Y-f, XIE X-y, LIN Q, QIU Z-w, YE J-x, FU C-l.？Efficacy of Achyranthis Bidentatae Radix Against？Cartilage Degeneration in Osteoarthritis: An Exploration？Based on Computer Simulation. Chinese Journal of？Experimental Traditional Medical Formulae. 2021:148-55.
Pitcher T, Sousa-Valente J, Malcangio M. The？monoiodoacetate model of osteoarthritis pain in the？mouse. Journal of visualized experiments: JoVE.？2016(111).
Sokolove J, Lepus CM. Role of inflammation in the？pathogenesis of osteoarthritis: latest findings and？interpretations. Therapeutic advances in musculoskeletal？disease. 2013;5(2):77-94.
Gabay C. Interleukin-6 and chronic inflammation.？Arthritis research & therapy. 2006;8(2):1-6.
Jiang W, Jin Y, Zhang S, Ding Y, Huo K, Yang J, et al.？PGE2 activates EP4 in subchondral bone osteoclasts to？regulate osteoarthritis. Bone research. 2022;10(1):27.

상세보기
Zhu J, Zhen G, An S, Wang X, Wan M, Li Y, et al.？Aberrant subchondral osteoblastic metabolism modifies？NaV1. 8 for osteoarthritis. Elife. 2020;9:e57656.

상세보기
Bi X. Correlation of serum cartilage oligomeric matrix？protein with knee osteoarthritis diagnosis: a？meta-analysis. Journal of orthopaedic surgery and？research. 2018;13(1):1-8.
Verma P, Dalal K. Serum cartilage oligomeric matrix？protein (COMP) in knee osteoarthritis: a novel？diagnostic and prognostic biomarker. Journal of？Orthopaedic Research. 2013;31(7):999-1006.

상세보기
Fernandes F, Pucinelli MLC, Da Silva N, Feldman D.？Serum cartilage oligomeric matrix protein (COMP) levels？in knee osteoarthritis in a Brazilian population: clinical？and radiological correlation. Scandinavian journal of？rheumatology. 2007;36(3):211-5.

상세보기
Blumenfeld O, Williams F, Hart D, Spector T, Arden N,？Livshits G. Association between cartilage and bone？biomarkers and incidence of radiographic knee？osteoarthritis (RKOA) in UK females: a prospective？study. Osteoarthritis and Cartilage. 2013;21(7):923-9.

상세보기
El-Arman MM, El-Fayoumi G, El-Shal E, El-Boghdady I,？El-Ghaweet A. Aggrecan and cartilage oligomeric matrix？protein in serum and synovial fluid of patients with？knee osteoarthritis. HSS Journal®. 2010;6(2):171-6.

상세보기
Henrotin Y. Osteoarthritis in year 2021: biochemical？markers. Osteoarthritis and cartilage. 2022;30(2):237-48.
Mohan G, Perilli E, Kuliwaba JS, Humphries JM,？Parkinson IH, Fazzalari NL. Application of in vivo？micro-computed tomography in the temporal？characterisation of subchondral bone architecture in a？rat model of low-dose monosodium iodoacetate-induced？osteoarthritis. Arthritis research & therapy. 2011;13:1-14.
Min G-Y, Park J-M, Joo I-H, Sim B-Y, Choi H-J, Kim？H-Y, et al. Effects of Chondroitin on Blood Related？Pathologic Factor and Weight Bearing in MIA？Osteoarthritis Model. 2019.
Joo I-H, Kim D-H. Effects of Yeonsan-Ogye Egg on？MIA-induced Osteoarthritis Rat. The Korea Journal of？Herbology. 2017;32(6):63-9.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증