[논문]Monosodium iodoacetate로 유발된 골관절염 쥐에 유향(乳香) 성분 3-Acetyl-11-Keto-Beta-Boswellic Acid의 연골보호 및 항염증 효과

김민주; 신미래; 최학주; 박해진; 최황용; 김화영; 노성수

doi:10.6116/kjh.2022.37.5.27.

Monosodium iodoacetate로 유발된 골관절염 쥐에 유향(乳香) 성분 3-Acetyl-11-Keto-Beta-Boswellic Acid의 연골보호 및 항염증 효과
3-Acetyl-11-Keto-Beta-Boswellic Acid from Boswellia serrata Attenuates Monosodium Iodoacetate-induced Osteoarthritis by Chondroprotective and Anti-inflammatory Effects 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.5, 2022년, pp.27 - 35

김민주 (대구한의대학교 한의과대학 본초학교실) , 신미래 (대구한의대학교 한의과대학 본초학교실) , 최학주 (대구한의대학교 한의과대학 본초학교실) , 박해진 (DHU 바이오융복합시험센터) , 최황용 (주영엔에스(주)) , 김화영 (주영엔에스(주)) , 노성수 (대구한의대학교 한의과대학 본초학교실)

Abstract ▼ AI-Helper

Objectives : 3-Acetyl-11-keto-𝛽-boswellic acid (AKBA) is a major active compound in Boswellia serrata. We investigated the arthritic changes following AKBA administration in monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : All rats were randomly divided into five groups: Normal, Control, INDO (indomethacin 2 mg/kg treated), AKBA30 (AKBA 30 mg/kg treated), and AKBA60 (AKBA 60 mg/kg treated); drugs were given 2 weeks before MIA injection. For all groups except the normal group, 50 µL of sterile saline with MIA (80 mg/mL) was injected into the right knee joint 2 weeks after drug administration. The drug administration was continued for 4 weeks from 1 week after osteoarthritis induction. The histomorphological changes of knee joint cartilage were observed by H&E staining. Also, the levels of glycosaminoglycan (GAG), cartilage oligomeric matrix protein (COMP), 5-lipoxygenase (5-LOX), 5-LOX-activating protein (FLAP), and leukotriene B4 (LTB4) in the knee joint were determined by the ELISA kits. The expressions of mitogen-activated protein kinases (MAPKs), inflammatory cytokines, and matrix metalloproteinases (MMPs) in knee joint were detected by Western blot. Results : Data show that levels of 5-LOX, FLAP, LTB4, and COMP were downregulated significantly in the AKBA treated groups when compared to those in the Control group. On the other hand, GAG levels were significantly elevated. As a result of Western blot, the AKBA-treated groups significantly inhibited phosphorylation of MAPKs. In addition, significant downregulation of the expression of inflammatory cytokines and MMPs was found in the AKBA-treated groups. Conclusion : Our findings suggest that administration of AKBA could exert better chondroprotective and anti-inflammatory effects for MIA-induced osteoarthritis rats.

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표/그림 (8)

그림 Fig. 1.Effects of AKBA on the hind paw weight bearing distribution in MIA-induced osteoarthritis rats. All data are expressed mean ±SD (n = 9 rats per group). Significance: ^###p < 0.001 compared vs. Normal group and ***p < 0.001 vs. Control group by LSD test.
그림 Fig. 2. Effects of AKBA on the levels of GOT and GPT in the serum of MIA-induced osteoarthritis rats. Levels of (A) GOT and (B) GPT in serum measured by ELISA. All data are expressed mean±SD (n = 9 rats per group). Significance: ^#p < 0.05, ^###p < 0.001 compared vs. Normal group and *p < 0.05 vs. Control group by LSD test.
그림 Fig. 3. Effects of AKBA on the levels of GAG and COMP in the knee joint of MIA-induced osteoarthritis rats. Levels of (A) GAG and (B) COMP in knee joint measured by ELISA. All data are expressed mean±SD (n = 9 rats per group). Significance: ^###p < 0.001 compared vs. Normal group and *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control group by LSD test.
그림 Fig. 4. Effects of AKBA on the levels of inflammation-related factors in the knee joint of MIA-induced osteoarthritis rats. Levels of (A) 5-LOX, (B) FLAP, and LTB4 in knee joint measured by ELISA. All data are expressed mean±SD (n = 9 rats per group). Significance: ^###p < 0.001 compared vs. Normal group and *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control group by LSD test.
그림 Fig. 5. Effects of AKBA on activities of MAPKs in the knee joint of MIA-induced osteoarthritis rats. Expression of ERK, JNK, and p38 in knee joint measured by western blot. All data are expressed mean±SD (n = 9 rats per group). Significance: ^###p < 0.001 compared vs. Normal group and *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control group by LSD test.
그림 Fig. 6. Effects of AKBA on activities of inflammatory cytokines in the knee joint of MIA-induced osteoarthritis rats. Expression of TNF-α, IL-1β, and IL-6 in knee joint measured by western blot. All data are expressed mean±SD (n = 9 rats per group). Significance: ^###p < 0.001 compared vs. Normal group and *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control group by LSD test.
그림 Fig. 7. Effects of AKBA on activities of MMPs in the knee joint of MIA-induced osteoarthritis rats. Expression of MMP-3 and MMP- 13 in knee joint measured by western blot. All data are expressed mean±SD (n = 9 rats per group). Significance: ^###p < 0.001 compared vs. Normal group and *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control group by LSD test.
그림 Fig. 8. Histopathological features of knee joint tissue of MIAinduced osteoarthritis rats. Representative photographs of knee joint tissues stained with hematoxylin&eosin (H&E, ×40 magnification). Scale bars = 300 ㎛

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