[논문]플라보노이드의 세포외 기질 관련 유전자 발현에 관한 연구

이재은; 박세연

doi:10.12925/jkocs.2023.40.5.1010

플라보노이드의 세포외 기질 관련 유전자 발현에 관한 연구
A Study on the Expression of Genes Related to Extracellular Substrates of Flavonoids 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.40 no.5, 2023년, pp.1010 - 1021

이재은 (오산대학교 뷰티코스메틱계열) , 박세연 (동덕여자대학교 자연정보대학 응용화학전공)

초록
AI-Helper

본 연구에서는 flavonoid apigenin과 baicalein의 세포 실험으로 콜라겐 회복효과를 확인하고 전사체 비교 분석을 통하여 collagen 회복 효과를 뒷받침할 수 있는 결과를 제안하고자 한다. 연구 결과 apigenin과 baicalein은 HS68 세포주에서 UV에 의해 손상된 I형 collagen 회복에 효과를 보였으며, 두 물질 모두에서 ECM 관련 유전자들의 발현 변화를 확인하였다. ECM을 조절하는 공통적인 메커니즘을 보일 뿐 아니라 각각 다른 범주의 유전자들을 변화시키는 결과도 보여서, 세포에 다양한 영향을 미치는 것으로 예측할 수 있었다.

Abstract ▼ AI-Helper

In this study, we would like to confirm the collagen recovery effect through cell experiments of the flavonoid apigenin and baicalein and propose results that can support the collagen recovery effect through comparative transcriptome analysis. As a result of the study, it was confirmed that apigenin and baicalein were effective in recovering type I collagen damaged by UV in the HS68 cell line, and that both substances resulted in changes in the expression of ECM-related genes. Not only did it show a common mechanism for regulating the ECM, but it also showed the results of changing different categories of genes, making it possible to predict that it would have various effects on cells.

주제어

표/그림 (8)

그림 Fig. 1. Protective effect of flavonoids against UV-induced oxidative stress. HS68 cells were exposed to UVB and treated at concentrations of 10, 20, and 50 μg/mL. The data shown are expressed as mean±S.D. from three repeated experiments with similar results.
그림 Fig. 2. Cytotoxicity effects of apigenin and baicalein in cell lines Hs-68. Apigenin and baicalein were treated with HS68 cell line at concentrations of 10 μM, 20 μM, and 50 μM, and cell viability was measured.
그림 Fig. 3. Apigenin and baicalein effects on collagen synthesis. Enzyme-linked immunosorbent assay (ELISA) was used to determine the amount of procollagen type I secreted from skin cells as a precursor form of collagen protein. HS68 cells were irradiated with ultraviolet (UV) light at 450 nm and then treated with apigenin and baicalein at a concentration of 50 μ M each(*p<0.05).
그림 Fig. 4. ECM(extractal matrix) related gene expression of apigenin and baicalein. Among the genes whose expression changed compared to the control group, only the gene categories related to ECM (extracellular matrix) were identified in the drawn gene clustering heatmap.
표 Table 1. Genes in which Apigenin and baicalein have been changed in common upward or downward. Fold change ≥4, Fold change ≤4
그림 Fig. 5. Gene category chart for apigenin and baicalein. The results of comparing the categories of gene functions regulated by apigenin and baicalein and the gene category chart results of genes with decreased or increased expression in each individual were shown.
그림 Fig. 6. Gene clustering chart related to apigenin, baicalein.
그림 Fig. 7. Apeigenin and baicalein simultaneously increased 41 gene string networks. To analyze the correlation between genes simultaneously increased in apigenin and baicalein, the results of a network of protein-protein interactions (PPIs) were drawn at https://string-db.org/.

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