[논문]난소적출로 유발된 갱년기장애 마우스 모델에서 육인주(毓麟珠) 열수 추출물의 증상 개선 효과

최유정; 김동철

doi:10.15204/jkobgy.2023.36.4.001

난소적출로 유발된 갱년기장애 마우스 모델에서 육인주(毓麟珠) 열수 추출물의 증상 개선 효과
Yuklinzu Aqueous Extracts Ameliorate Experimental Climacterium Symptoms Induced by Ovariectomy in Mouse 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.36 no.4, 2023년, pp.1 - 20

최유정 (대구한의대학교 한의과대학 한방부인과교실) , 김동철 (대구한의대학교 한의과대학 한방부인과교실)

Abstract ▼ AI-Helper

Objectives: The object of this study was to observe the complex anti-climacterium potentials of Yuklinzu aqueous extracts (YLZ), using bilateral ovariectomy (OVX) female ddY mice similar to women postmenopausal symptoms, as including cardiovascular diseases, obesity, hyperlipidemia, osteoporosis and hepatic steatosis. Methods: In order to evaluate anti-climacterium effects of YLZ, six groups of mice were used; sham control, OVX control, 17β-estradiol, YLZ 500, 250 and 125 mg/kg treated groups. Since 28 days after bilateral OVX surgery, YLZ were administered orally for 84 days, once a day. And then we evaluated anti-climacterium effects divided into five categories; estrogenic effects, anti-obesity, hypolipidemic effects, hepatoprotective effects and anti-osteoporotic effects. The results of YLZ were compared with 17β-estradiol 0.03 ㎍/head/day subcutaneous treated OVX mice. Results: As a result of OVX, obvious changes related to the estrogen-deficient menopausal symptoms - obesity, hyperlipidemia, hepatic steatosis and osteoporosis were displayed in mice. However, these menopausal symptoms induced by OVX were significantly inhibited by 84 days of consecutive treatment of 17β-estradiol, YLZ 500, 250 and 125 mg/kg, respectively. Especially, YLZ showed obvious dose-dependent inhibitory activities on the OVX-induced climacterium changes in mice, and YLZ 500 mg/kg showed comparable inhibitory effects against menopausal symptoms in comparison with those of 17β-estradiol 0.03 ㎍/head/day subcutaneous treatment. Conclusions: The results suggest that oral administration of YLZ 500, 250 and 125 mg/kg has obvious dose-dependent favorable anti-climacterium effects in OVX mice. Especially, YLZ 500 mg/kg showed comparable inhibitory effects against menopausal symptoms in comparison with those of 17β-estradiol 0.03 ㎍/head/day subcutaneous treatment.

주제어

표/그림 (22)

그림 Fig. 1. Experimental design.
표 Table 1. Experimental Designs
표 Table 2. Composition of YLZ
그림 Fig. 2. Body weight changes. ED : Reference drug, 17β-estradiol 0.03 μg/head subcutaneous treatment; All animals were overnight fasted before OVX, first administration and sacrifice, respectively (†)
표 Table 3. Body Weight Gain
표 Table 4. Food Consumptions
그림 Fig. 3. Representative abdominal fat pad gross image. Scale bar=12 mm
그림 Fig. 4. Representative uterus gross image. Scale bar=13 mm
표 Table 5. Changes on the Abdominal Fat Pad, Uterus and Liver Weights in OVX Mice
그림 Fig. 5. Serum estradiol levels.
표 Table 6. Right Femur Weights
표 Table 7. Serum Biochemistry - AST, ALT, TC, LDL, HDL and TG Levels
그림 Fig. 6. Serum osteocalcin levels.
그림 Fig. 7. Serum bALP levels.
그림 Fig. 8. Representative whole body DEXA image.
그림 Fig. 9. Representative abdominal fat pads histological images. All hematoxylin and eosin stain, Scale bars =120 μm
표 Table 8. Fat Density, Bone Mineral Density and Femur Strength
그림 Fig. 10. Representative left uterus horn histological images. L : Lumen, MU : Mucosa, ML : Muscular layer, UG : Uterine gland, All Hematoxylin and eosin stain, Scale bars=120 μm
그림 Fig. 11. Representative liver left lateral lobe histological images. CV : Central vein, PT : Portal Triad, Scale bars=120 μm
표 Table 9. Histomorphometry - Abdominal Fat Pads, Uterus and Liver
그림 Fig. 12. Representative left femur histological images.
표 Table 10. Histomorphometry - Bone Mass and Resorption of Left Femur

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