[논문]고지방식이로 유도된 비만 마우스에서 정향(丁香)의 항비만 효과

안희연; 노성수; 신미래

doi:10.6116/kjh.2024.39.1.11

고지방식이로 유도된 비만 마우스에서 정향(丁香)의 항비만 효과
The Anti-Obesity Activity of Syzygium aromaticum L. in High-Fat Diet-induced Obese Mice 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.39 no.1, 2024년, pp.11 - 21

안희연 (대구한의대학교 한의학과 본초약리학교실) , 노성수 (대구한의대학교 한의학과 본초약리학교실) , 신미래 (대구한의대학교 한의학과 본초약리학교실)

Abstract ▼ AI-Helper

Objectives : This study aims to analyze the anti-obesity effect of Syzygium aromaticum L. (SA) in obese mice made by a 60% high-fat diet (HFD). Methods : The antioxidant activities of SA were evaluated in vitro. To assess the anti-obesity effect of SA, male C57BL/6 mice were divided into five groups: Normal, Control, GC100 (Garcinia cambogia 100 mg/kg/day), SA100 (SA 100 mg/kg/day), SA200 (SA 200 mg/kg/day). All groups underwent a 6-week regimen of HFD and oral administration, except for the Normal group. Subsequently, we performed blood analysis, western blotting, and histopathological staining. Results : SA demonstrated effectiveness in antioxidant measurements. SA treatment resulted in a significant decrease in body weight gain, along with reductions in liver and epididymal fat weights. Serum triglyceride (TG), total cholesterol (TC), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and leptin levels were reduced with SA treatment. Moreover, in the SA100 group, the reduction of both TG and TC synthesis was caused by inhibiting the sterol regulatory element-binding transcription factor 1 (SREBP-1) and sterol regulatory element-binding transcription factor 2 (SREBP-2) through the Sirtuin 1 (Sirt1)/phospho-AMP-activated protein kinase (p-AMPK) pathway. Furthermore, SA treatment at a dose of 100 mg/kg reduced the accumulation of lipid droplets in the liver and the adipocyte size of the epididymal fat. Conclusion : Our research reveals the anti-obesity effects of SA by demonstrating its ability to inhibit body weight gain and lipid accumulation, suggesting that SA might be promising for obesity treatment.

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표/그림 (11)

표 Table 1. The ingredients of 60 kcal% high-fat diet.
표 Table 2. Total polyphenol and total flavonoid content in SA.
표 Table 3. DPPH and ABTS radical scavenging activities in SA.
그림 Figure 1. Effects of TG and TC in serum by SA treatment. To assess the potential influence of SA supplementation on circulating lipid levels, serum lipid profile such as TG and TC was conducted. TG, triglyceride; TC, total cholesterol. All values are expressed as mean ± SEM. ^###p<0.001 vs. Normal group. *p<0.05 and **p<0.01 vs. Control group.
그림 Figure 2. Effects of GOT and GPT in serum by SA treatment. HFD intake induces liver injury. We examined whether SA ameliorated biomarkers of liver injury, such as GOT and GPT. All values are expressed as mean ± SEM. *p<0.05 and **p<0.01 vs. Control group.
표 Table 4. Initial and final body weight, body weight change, liver weight, and epididymal fat weight by SA treatment.
그림 Figure 3. Effects of leptin in serum by SA treatment. Level of serum leptin was measured using a commercial kit at the end of experimental treatment. All values are expressed as mean ± SEM. ^###p<0.001 vs. Normal group. *p<0.05, **p<0.01, and ***p<0.001 vs. Control group. ^$$p<0.01 vs. GC100 group.
그림 Figure 4. Effects of Sirt1 and p-AMPK protein expressions induced by SA treatment in the liver tissue. Protein lysates from the liver were used for western blot analyses to evaluate Sirt1 and p-AMPK expressions. All values are expressed as mean ± SEM. ^##p<0.01 vs. Normal group. *p<0.05 and ***p<0.001 vs. Control group. ^$$p<0.01 vs. GC100 group.
그림 Figure 5. Effects of SREBP-1 and ACC protein expressions induced by SA treatment in the liver tissue. Protein lysates from the liver were used for western blot analyses to evaluate SREBP-1 and ACC expressions. All values are expressed as mean ± SEM. ^#p<0.05 and ^##p<0.01 vs. Normal group. *p<0.05, **p<0.01, and ***p<0.001 vs. Control group.
그림 Figure 6. Effects of SREBP-2 and HMGCR protein expressions induced by SA treatment in the liver tissue. Protein lysates from the liver were used for western blot analyses to evaluate SREBP-2 and HMGCR expressions. All values are expressed as mean ± SEM. ^###p<0.001 vs. Normal group. *p<0.05 and **p<0.01 vs. Control group.
그림 Figure 7. Effect of histopathological change tissues by SA treatment of high-fat diet (×200, scale bar 50 μm). (A) The representative photomicrographs of liver tissues sections stained by H&E staining, (B) The representative photomicrographs of liver tissues sections stained by Oil Red O staining, (C) The representative photomicrographs of epididymis fat tissues sections stained by H&E staining. Normal, normal mice; Control, the obese mice; GC100, the obese mice were treated with Garcinia cambogia 100 ㎎/㎏ body weight; SA100, the obese mice were treated with Syzygium aromaticum L. 100 ㎎/㎏ body weight; SA200, the obese mice were treated with Syzygium aromaticum L. 200 mg/kg body weight.

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