[논문]한방 추출물의 역류성 식도염 점막보호 효과에 대한 스크리닝

정일하; 김민주; 신미래; 노성수

doi:10.6116/kjh.2024.39.1.39

[국내논문] 한방 추출물의 역류성 식도염 점막보호 효과에 대한 스크리닝
Screening for Mucosal Protective Effects of Various Korean Herbal Medicine Extracts in Gastroesophageal Reflux Disease

大韓本草學會誌 = The Korea journal of herbology, v.39 no.1, 2024년, pp.39 - 47

정일하 (대구한의대학교 한의학과 본초약리학교실) , 김민주 (대구한의대학교 간질환한약융복합활용연구센터) , 신미래 (대구한의대학교 한의학과 본초약리학교실) , 노성수 (대구한의대학교 한의학과 본초약리학교실)

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Objectives : This study evaluates how various traditional Korean herbal medicines assess MUC5AC expression for esophageal mucosal defense and analyzes the associated mechanisms involved in inflammation. Methods : Forty types of traditional Korean herbal medicines were assessed for in vitro antioxidant activities, and the real-time PCR method was employed to analyze MUC5AC expression under pH 4.5 conditions in human esophageal epithelial cells (HET-1A). Eight types of Korean herbal medicines were evaluated for in vitro antioxidant activities, and Reactive oxygen specise (ROS) expression was analyzed under bile salt (480 𝜇M) and pH 5.5 conditions in human esophageal epithelial cells (HET-1A). Simulation experiments involving bile salts and acidity were conducted for one hour to assess the efficacy of four drugs, and the activities of Mitogen-activated Protein Kinase (MEK), Nuclear Factor Kappa B (NF-𝜅B), and Cyclooxygenase-2 (COX-2) were detected through Western blot analysis. Results : Compared to the Normal group, the Control group exhibited higher ROS generation. Such increased ROS levels were significantly reduced by four extracts: Citrus Unshius Pericarpium (CUP), Cnidium officinale Rhizoma (CR), Ginseng Radix (GR), and Linderae Radix (LR). The protein expression of COX-2 decreased with the treatment of LR, CUP, and CR. Particularly, CUP and CR exhibited superior effects compared to other groups in inhibiting the phosphorylation of NF-𝜅B. Conclusion : Based on the results obtained, we have identified drugs that inhibit oxidative stress and inflammation caused by bile acid in esophageal epithelial cells. Our future plans involve comparing and analyzing the efficacy of these herbal medicines through animal experiments.

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표/그림 (8)

표 Table 1. Lists of herbal medicines used for this study
표 Table 2. Primer sequences used for PCR analysis
그림 Fig. 1. Screening of Esophageal Cell Protective Effects of 20 Extracts on MUC5AC mRNA Expression in HET-1A Cells. The mRNA levels of up-regulated HET-1A cells treated with 100 μg/ ml concentration of extract for 1 hour followed by exposure to hydrochloric acid (pH 4.5) for 30 minutes. All values are expressed as mean ± SEM (n=3).
그림 Fig 2. Reactive oxygen species (ROS) and expression in HET-1A cells.Exposure to 480 μM bile acid at pH 5.5 decreased the levels of ROS in esophageal epithelial cells for 8 extracts. All data are expressed as means ± SEM (n=3). Significance:^###p < 0.001 vs. Normal, *p < 0.05, **p < 0.01 vs. Control ***p < 0.001 vs. Control.
그림 Fig. 3. Protein p-NF-κB and COX-2 were activated in HET-1A cells treated for 3hours with a solution of pH 5.5 + 480 μM bile acids (BA). Western analysis of HET-1A cells lysates with (A) p-NF-κB activation and (B) COX-2 expression in HET-1A cell. All data are expressed as means ±SEM (n=3). Significance:^###p < 0.001 vs. Normal, *p < 0.05 **p < 0.01 and ***p < 0.001 vs. Control.
그림 Fig. 4. Effects of 4 extract of Water and EtOH extracts on cell viability in HET-1A cells. The CCK-8 results are presented as the percentage of cell viability of the cells. (A) Cnidium Rhizome; CRW (B) Ginseng Radix; GRW, (C) Citri Unshius Pericarpium; CUPW, (D) Linderae Radix; LRW, and (E) Aucklandiae Radix; AuRW. All data were performed in triplicate and expressed as mean ± SEM.
그림 Fig 5. Acidic bile salt increased the expression of p-NF-κB in HET-1A cells through western analysis. HET-1A cells were seeded overnight at a density of 1 × 10⁶ cells per well and the following day were untreated or treated for 3 h with pH 5.5 + 480 μM bile acids (BA). (A) Cnidium Rhizome; CRW, (B) Ginseng Radix; GRW, (C) Citri Unshius Pericarpium; CUPW, and (D) Linderae Radix; LRW. All values are expressed as mean ± SEM (n=3). Significance: ^###<0.001 vs. Normal, *p<0.05, **p<0.01 vs. Control ***p<0.001 vs. Control.Significance:^###p<0.001 vs. Normal, *p<0.05 **p <0.01 and ***p<0.001 vs. Control.
그림 Fig 6. Acidic bile salt increased the expression of COX-2 in HET-1A cells through western analysis. HET-1A cells were seeded overnight at a density of 1 × 10⁶ cells per well and the following day were untreated or treated for 3 h with pH 5.5 + 480 μM bile acids (BA). (A) Cnidium Rhizome; CRW, (B) Ginseng Radix; GRW, (C) Citri Unshius Pericarpium; CUPW, and (D) Linderae Radix; LRW. All values are expressed as mean ± SEM (n=3).Significance:^###p < 0.001 vs. Normal, *p < 0.05 **p < 0.01 and ***p < 0.001 vs. Control.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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