[논문]강황(薑黃) 계지(桂枝) 복합물이 RAW 264.7 세포에서 항염증 활성에 미치는 영향

최지; 박해진; 정일하; 김민주; 신미래; 노성수; 박순애; 김미림

doi:10.6116/kjh.2023.38.2.17

강황(薑黃) 계지(桂枝) 복합물이 RAW 264.7 세포에서 항염증 활성에 미치는 영향
Effects of Curcumae longae Rhizoma and Cinnamomi Ramulus Mixture on Anti-inflammatory Activities in Lipopolysaccharide-stimulated RAW 264.7 Cells 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.38 no.2, 2023년, pp.17 - 26

최지 (대구한의대학교 한방식품학과) , 박해진 (대구한의대학교 바이오융복합시험센터) , 정일하 (대구한의대학교 한의학과 본초약리학교실) , 김민주 (대구한의대학교 간질환한약융복합활용연구센터) , 신미래 (대구한의대학교 한의학과 본초약리학교실) , 노성수 (대구한의대학교 한의학과 본초약리학교실) , 박순애 (대구한의대학교 푸드케어학부) , 김미림 (대구한의대학교 푸드케어학부)

Abstract ▼ AI-Helper

Objectives : A persistent inflammatory response can cause diseases such as fibrosis, cancer, and allergies. This study aimed to investigate the anti-inflammatory activity of Curcumae longae Rhizoma and Cinnamomi Ramulus Mixture (CCM) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Methods : The total polyphenol and flavonoid contents of CCM were confirmed through an in vitro experiment. Also, radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and Hydroxyl were confirmed. Moreover, ferric reducing antioxidant power (FRAP) activity were confirmed. After, CCM (50, 100, and 200 ㎍/mL) were applied to 0.1 ㎍/mL LPS-stimulated RAW264.7 cells. The levels of nitric oxide (NO) and pro-inflammatory cytokines in the supernatant fraction were determined. Also, the expressions of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways were detected using Western blot. Results : As a result of in vitro experiments, there was an excellent antioxidant activity in CCM-treated cells. In addition, in RAW264.7 cells stimulated with LPS, the increased NO level was inhibited in a concentration-dependent manner by the treatment of CCM. In addition, inflammatory cytokines production were significantly inhibited in a concentration-dependent manner in CCM-treated group. CCM treatment significantly decreased the protein expressions of MAPKs. Moreover, the expressions of NF-κBp65 and cyclooxygenase-2 (COX-2) were significantly decreased when 200 mg/kg of CCM was applied, and phospho-inhibitor of nuclear factor kappa B-α (p-IκBα) and inducible nitric oxide synthase (iNOS) were significantly decreased at all concentrations treated with CCM. Conclusion : Our findings show that CCM exhibited excellent antioxidant activity and exhibited superior anti-inflammatory effect through the MAPKs and NF-κB pathways in LPS-stimulated RAW 264.7 macrophages.

주제어

표/그림 (10)

표 Table 1. Total polyphenol and total flavonoid content in CCM
표 Table 2. DPPH and ABTS radical scavenging activity of CCM
표 Table 3. FRAP activity of CCM
그림 Figure 1. Effects of CCM on RAW 264.7 cell viability. Curcumae longae Rhizoma and Cinnamomi Ramulus mixture; CCM. All data are expressed mean±SEM (n=3).
그림 Figure 2. Effects of CCM on the NO production in RAW 264.7 cells. Curcumae longae Rhizoma and Cinnamomi Ramulus mixture; CCM. All data are expressed mean±SEM (n=3). Significance: ^###p <0.001 compared vs. con group and ^***p <0.001 vs. LPS group by LSD test.
그림 Figure 3. Effects of CCM on the inflammatory cytokines levels in RAW 264.7 cells. (a) TNF-α and (b) IL-6. Curcumae longae Rhizoma and Cinnamomi Ramulus mixture; CCM. All data are expressed mean±SEM (n=3). Significance: ^###p <0.001 compared vs. con group and ^*p <0.05, ^**p <0.01, ^***p <0.001 vs. LPS group by LSD test.
표 Table 4. Hydroxyl radical scavenging activity of CCM
그림 Figure 4. Effect of CCM on MAPKs activation in RAW 264.7 cells. Curcumae longae Rhizoma and Cinnamomi Ramulus mixture; CCM. All data are expressed mean±SEM (n=3). Significance: ^###p <0.001 compared vs. con group and ^***p<0.001 vs. LPS group by LSD test.
그림 Figure 5. Effect of CCM on p-NF-kB and p-IkBα activation in RAW 264.7 cells. Curcumae longae Rhizoma and Cinnamomi Ramulus mixture; CCM. All data are expressed mean±SEM (n=3). Significance: ^###p<0.001 compared vs. con group and ^*p<0.05, ^***p <0.001 vs. LPS group by LSD test.
그림 Figure 6. Effect of CCM on iNOS and COX-2 activation in RAW 264.7 cells. Curcumae longae Rhizoma and Cinnamomi Ramulus mixture; CCM. All data are expressed mean±SEM (n=3). Significance: ^###p<0.001 compared vs. con group and ^*p<0.05, ^**p<0.01, ^***p<0.001 vs. LPS group by LSD test.

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저자의 다른 논문 :

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동의어: Packet Collision Rate

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