[논문]AMPK 활성화를 통한 중금속 유발 산화적 스트레스에 대한 비파엽의 세포 보호 효과

김민진; 김영은; 박선빈; 배수진; 박광일; 박선동; 김영우

doi:10.14374/hfs.2024.32.1.99

AMPK 활성화를 통한 중금속 유발 산화적 스트레스에 대한 비파엽의 세포 보호 효과
Cytoprotective effect of Eriobotrya japonica L. against the iron-induced oxidative stress through AMPK activation 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.32 no.1, 2024년, pp.99 - 109

김민진 (동국대학교 한의과대학 방제학교실) , 김영은 (동국대학교 한의과대학 방제학교실) , 박선빈 (동국대학교 한의과대학 방제학교실) , 배수진 (동국대학교 한의과대학 방제학교실) , 박광일 (경상대학교 수의과대학 생리학 실험실) , 박선동 (동국대학교 한의과대학 방제학교실) , 김영우 (동국대학교 한의과대학 방제학교실)

Abstract ▼ AI-Helper

Objectives : In this study, we investigated the cytoprotective effect of Eriobotrya japonica L. (EJ) extract against Arachidonic acid (AA)+iron-induced oxidative stress. Methods : To confirm the cytoprotective effect of EJ against AA+iron-induced oxidative stress in HepG2 cells, it was evaluated by MTT assay, immunoblot anaylsis, and Calcein-AM/propidium iodide (PI) staining. Additionally, the mechanism of action of the cytoprotective effect was evaluated through molecular mechanisms. Results : EJ (100 ㎍/mL) inhibited Arachidonic acid (AA)+iron-induced cell death in a concentration-dependent manner. It also inhibited AA+iron-induced mitochondrial dysfunction and ROS production. EJ activated the LKB1-AMPK signaling pathway. Conclusions : In conclusion, EJ has the ability to protect liver cells from oxidative stress, indicating that it is related to AMPK-LKB1 signaling pathways.

주제어

표/그림 (5)

그림 Fig. 1. MTT assay. Cell viability was assessed using an MTT assay. HepG2 cells were treated with Arachidonic acid (AA) (10 μM) for 12h, followed by iron (5 μM) for 3h. Eriobotrya japonica (EJ) (10, 30, 100 μg/mL) was pretreated with AA. The data represent the means ± S.D. of replicate experiments at least three times. Significance of statistical differences between each treatment group and the vehicle-treated control group (***, p < 0.001) and AA+iron-treated control group (###, p < 0.001) were verified.
그림 Fig. 2. Effect of EJ on AA + iron-induced cell death in HepG2.(A) Immunoblotting of cell death-related protein, Bcl-xl, caspase 3. HepG2 cells were treated with AA (10 μM) for 12 h, followed by iron (5 μM) for 3 h. EJ (100 μg/mL) was pretreated with AA. (B) Fluorescence image of HepG2 cells using calcein-AM/PI staining. HepG2 cells were treated with AA (10 μM) for 12 h, followed by iron (5 μM) for 3 h. EJ (100 μg/mL) was pretreated with AA. HepG2 cells were stained with 0.5 μg/mL calcein-AM and 0.5 μg/mL PI and incubated for 30 min.
그림 Fig. 3. Effect of EJ on AA+iron-induced mitochondrial dysfunction and ROS production in HepG2 cell. (A) FACS analysis of Rho 123 staining. HepG2 cells were treated with AA (10 μM) for 12h, followed by iron (5 μM) for 3h. EJ (100 μg/mL) was pretreated with AA. The cells were incubated with Rho 123 (0.05 μg/mL) for 1h after the iron treatment. (B) ROS production measurements. The cells were incubated with DCFH-DA (10 μM) for 30 min after iron treatment. The data represent the means ± S.D. of replicate experiments at least three times (***p < 0.001 vs. control group; ##p < 0.01, ###p < 0.001 vs. AA+iron-treated group).
그림 Fig. 4. Immunoblot analysis for key signaling proteins of LKB1-AMPK signaling pathway molecules in (A) HepG2 cell and (B) Huh7 cell. HepG2 cell and Huh7 cell were incubated with EJ (100 μg/mL) for indicated periods. β-actin was used as a loading control. The graph shows relative protein levels in HepG2 cells. The data represent the means ± S.D. of replicate experiments at least three times (*p < 0.05 vs. control group).
그림 Fig. 5. Immunoblot analysis for key signaling proteins of YAP signaling pathway molecules in (A) HepG2 cell and (B) Huh7 cell. HepG2 cell and Huh7 cell were incubated with EJ (100 μg/mL) for indicated periods. β-actin was used as a loading control.

참고문헌 (24)

Park YC, Park HM, Lee SD. Inducible Mechanisms for Hepatotoxicity caused by Traditional Korean Medicines in a view of Toxicology. Journal of Korean Medicine. 2011;32(4):48-67.？
Kim DJ. Review : The Assessment of Toxic Liver Injury. Korean Journal of Gastroenterol. 2009;53(1):5-14.？
Yang YM, Hyun JW, Lim KH, Sung MS, Kang SS, Park WH, et al. Antineoplastic effect of extracts from traditional medicinal plants and various plants (III). Korean Journal of Pharmacognosy. 1996;27:105-110.？
Sunami E, Tsuno N, Osada T, Saito S, Kitayama J, Tomozawa S, et al. MMP-1 is a prognostic marker for hematogenous metastasis of colorectal cancer. The Oncologist. 2000;5(2):108-114.？

상세보기
Rani V, Deep G, Singh RK, Palle K, Yadav U. Oxidative stress and metabolic disorders: Pathogenesis and therapeutic strategies. Life Sciences. 2016;148:183-193.？

상세보기
Cho SH, Choi YJ, Rho CW, Choi CY, Kim DS, Cho SH. Reactive oxygen species and cytotoxicity of bamboo (Phyllostachys pubescens) sap. Korean Journal of Food Preservation. 2008;15(1):105-110.？
Puntarulo S. Iron, oxidative stress and human health. Molecular aspects of medicine. 2005;26(4-5):299-312.？
Lim DG. Oxidative stress; reactive oxygen species and nitric oxide. Acute and Critical Care. 2004;19(2):81-85.？
Kang SW. Role of reactive oxygen species in cell death pathways. Hanyang Medical Reviews. 2013;33(2):77-82.？

상세보기
Jang MH, Seo HL, Kim SC, Kim YW. Effect of Prunellae Spica on Oxidative Stress and Mitochondrial Dysfunction in the Hepatocyte. J Physiol and Pathol Korean Med. 2016;30(1):20-26.？
Seo HL, Lee JH, Jang MH, Kwon YW, Cho IJ, Kim KJ et al. Mitochondria protection of Sparganii Rhizoma against oxidative stress in heptocytes. Herbal Formula Science, 2015;23(2):15-22.？
Bae YI, Seo KI, Park SK, Shim KH. Loquat (Eriobotrya japonica Lindl.) Leaf Tea Processing and Its Physicochemical Properties. Korean Journal of food preservation. 1998;5(3):262-269.？
Kim SJ, Park JO, Park SN. Antioxidative Effect and Component Analysis of Eriobotrya japonica Leaf Extracts. Journal of the Soc. Cosmet. Sci. Korea. 2012;38(1):57-65.？
Choi HY, Jeggal KH, Kim YW, Lee JW, Jo SA, Cho IJ, et al. Cytoprotective effects of Artemisia princeps extract through inhibition of mitochondrial dysfunction. Herbal Formula Science. 2013;21(2):63-71.？

원문보기 상세보기
Jang EH, Kim SC, Cho IJ, Kim YW. Akebiae Caulis inhibits oxidative stress through AMPK activation. Journal Physiol and Pathol Korean Med. 2015;29(1):18-26.？
Balboa MA, Balsinde J. Oxidative stress and arachidonic acid mobilization. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids. 2006;1761(4):385-391.？

상세보기
Kuang F, Liu J, Tang D, Kang R. Oxidative damage and antioxidant defense in ferroptosis. Frontiers in Cell and Developmental Biology. 2020;8, 586578.？

상세보기
Lee SG, Yu MH, Lee SP, Lee IS. Antioxidant activities and induction of apoptosis by methanol extracts from avocado. Journal of the Korean Society of Food Science and Nutrition. 2008;37(3):269-275.？

원문보기 상세보기
Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH. Effect of Lentinus edodes and Pleurotus eryngii Extracts on Proliferation and apoptosis in human colon cancer cell lines. Journal of Korean Soc. Food Sci Nutr. 2003;32(2):217-222.？
Asadi M, Taghizadeh S, Kaviani E, Vakili O, Taheri-Anganeh M, Tahamtan M, et al. Caspase-3: structure, function, and biotechnological aspects. Biotechnology and Applied Biochemistry. 2022;69(4):1633-1645.？

상세보기
Porter AG, Janicke RU. Emerging roles of caspase-3 in apoptosis. Cell death & differentiation. 1999;6(2):99-104.？

상세보기
Li M, Wang D, He J, Chen L, Li H. Bcl-XL: A multifunctional anti-apoptotic protein. Pharmacological research. 2020;151:104547.？

상세보기
Zhou F, Yang Y, Xing D. Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis. The FEBS journal. 2011;278(3):403-413.？

상세보기
Hardie DG, Ross FA, Hawley SA. AMPK: a nutrient and energy sensor that maintains energy homeostasis. Nature reviews Molecular cell biology. 2012;13(4):251-262.

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

AMPK 활성화를 통한 중금속 유발 산화적 스트레스에 대한 비파엽의 세포 보호 효과
Cytoprotective effect of Eriobotrya japonica L. against the iron-induced oxidative stress through AMPK activation 원문보기

Abstract ▼ AI-Helper

주제어

표/그림 (5)

표/그림 (5)

참고문헌 (24)

이 논문을 인용한 문헌

저자의 다른 논문 :

관련 콘텐츠

원문 보기

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

AMPK 활성화를 통한 중금속 유발 산화적 스트레스에 대한 비파엽의 세포 보호 효과 Cytoprotective effect of Eriobotrya japonica L. against the iron-induced oxidative stress through AMPK activation 원문보기

Abstract ▼ AI-Helper

주제어

표/그림 (5) 모든 표/그림 보기

표/그림 (5) 슬라이드로 보기

참고문헌 (24)

이 논문을 인용한 문헌

저자의 다른 논문 :

박선동 (102)

관련 콘텐츠

원문 보기

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

AMPK 활성화를 통한 중금속 유발 산화적 스트레스에 대한 비파엽의 세포 보호 효과
Cytoprotective effect of Eriobotrya japonica L. against the iron-induced oxidative stress through AMPK activation 원문보기

표/그림 (5)

표/그림 (5)