[논문]양춘사 추출물이 HepG2 세포에서 콜레스테롤 합성에 미치는 효과

김하림; 김예슬; 최한별; 우수현; 권강범

doi:10.15188/kjopp.2024.02.38.1.27

[국내논문] 양춘사 추출물이 HepG2 세포에서 콜레스테롤 합성에 미치는 효과
Effects of Amomum Villosum Extracts on Cholesterol Synthesis in HepG2 Cells 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.38 no.1, 2024년, pp.27 - 31

김하림 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 김예슬 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 최한별 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 우수현 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오) , 권강범 (원광대학교 한의과대학 생리학교실 및 (주)일원바이오)

Abstract ▼ AI-Helper

Dried fruits of Amomum villosum Lour. have been used an korean medicine to treat digestive diseases for a long time. It has been reported that Amomum villosum extracts(AVE) have effects for diabetes and steatosis in experimental models. But we did not find the report about the cholesterol synthesis inhibition effects of AVE. The objective of this study is to clarify the inhibitory effect of AVE against oleic acid and glucose-induced hypercholesterolemia in HepG2 cells. The results show that AVE had a significant inhibitory effect against oleic acid and glucose-induced cholesterol accumulation. Those effects seem to be caused by inhibition of AVE on oleic acid and glucose-induced decrease of HMG CoA reductase, which is the rate-limiting enzyme for cholesterol biosynthesis in liver. It is believed that the results of this study can provide basic data for the drug and functional food development of hypercholesterolemia treatments.

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표/그림 (6)

그림 Fig. 1. Effects of Amomum villosum extracts (AVE) on cell viability in HepG2 cells. Cells were treated with 20uM simvastatin (SIM) and various concentrations of AVE for 2 days. Data were analyzed as EZ-cytox kit. Values are means ± SD; n=5.
표 Table 1. Sequences and Accession Numbers for Primer, Forward and Reverse, Used in Real-Time PCR.
그림 Fig. 2. Effects of Amomum villosum extracts (AVE) on total cholesterol concentrations in HepG2 cells. Cells were treated with 800uM oleic acid and 10mM glucose in the presence of various concentrations of AVE for 16 h. Total cholesterol concentrations were analyzed as follows in material and methods. Values are means ± SD; n=5. ##p<0.01 vs. CON, *p<0.05, **p<0.01 vs. OAG (800uM oleic acid + 10mM glucose). CON; control, SIM; 20uM simvastatin.
그림 Fig. 3. Effects of Amomum villosum extracts (AVE) on lipid concentrations in HepG2 cells. Cells were treated with 800uM oleic acid and 10mM glucose in the presence of various concentrations of AVE for 16 h. Total lipid concentrations were analyzed by using Oil Red O stain. Values are means ± SD; n=5. ##p<0.01 vs. CON, *p<0.05, **p<0.01 vs. OAG (800uM oleic acid + 10mM glucose). CON; control, SIM; 20uM simvastatin.
그림 Fig. 4. Effects of Amomum villosum extracts (AVE) on cholesterol synthesis-related protein expressions in HepG2 cells. Cells were treated with 800uM oleic acid and 10 mM glucose in the presence of various concentrations of AVE for 16h. Protein expressions were analyzed by Western blotting. SIM; 20uM simvastatin. SREBP-2; Sterol regulatory element binding protein-2, HMGCR; HMG-CoA reductase, OAG; 800 uM oleic acid + 10mM glucose.
그림 Fig. 5. Effects of Amomum villosum extracts (AVE) on cholesterol synthesis-related mRNA expressions in HepG2 cells. Cells were treated with 800uM oleic acid and 10 mM glucose in the presence of various concentrations of AVE for 16h. mRNA expressions were analyzed by Real time OCR. Values are means±SD; n=5. ##p<0.01 vs. CON, *p<0.05, **p<0.01 vs. OAG (800uM oleic acid + 10mM glucose). CON; control, SIM; 20uM simvastatin. SREBP-2; Sterol regulatory element binding protein-2, HMGCR; HMG-CoA reductase.

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