[논문]한반도 고래불 해안사구에 자생하는 혼합 염생식물군락 근권세균의 분포 및 다양성 고찰 : 해안사구 보전을 위한 접근

박종명; 홍지원; 이기은; 김종국; 유영현

doi:10.15433/ksmb.2024.16.1.001

[국내논문] 한반도 고래불 해안사구에 자생하는 혼합 염생식물군락 근권세균의 분포 및 다양성 고찰 : 해안사구 보전을 위한 접근
Distribution and diversity of rhizosphere bacteria of mixed halophytes vegetation native to the Goraebul sand dune, Korea : Approaches to coastal dune conservation

한국해양바이오학회지 = Journal of marine bioscience and biotechnology, v.16 no.1, 2024년, pp.1 - 18

박종명 (인천상수도사업본부 맑은물연구소) , 홍지원 (경북대학교 수소 및 신재생에너지학과) , 이기은 (국립생물자원관 생물종다양성연구과) , 김종국 (경북대학교 생명과학부) , 유영현 (국립생물자원관 생물종다양성연구과)

Abstract ▼ AI-Helper

Coastal dunes must be conserved. Their native halophytes support coastal geography while their symbiotic microorganisms help vegetation thrive. The Goraebul coast has the largest, well-conserved dune system on the East Sea of the Korean Peninsula due to a climax mixed halophyte (C. soldanella, C. kobomugi, and E. mollis) vegetation support. This study identified rhizobacteria and their diversity in mixed halophyte communities unique to Goraebul. Five phyla, 12 genera, and 21 species were identified based on 16S rDNA sequences from 65 isolates. The phylum Bacillota, class Bacillota, order Bacillales, and family Bacillaceae were identified, with Bacillus as the dominant genus (46.15%). The richness and Shannon's diversity were higher at the species than at the genus level due to the dominance of Bacillus; however, various Bacillus species (7) were identified. Therefore, the climax mixed vegetation adapted to the Goraebul coast may exert natural selection pressure in favor of the common characteristics of Bacillus. However, despite this advantage, the Shannon equitability (0.86), Simpson (0.08), and Shannon diversity (2.79) indexes indicate a stable rhizosphere cluster and the climax mixed vegetation is affected by symbiotic relationships between healthy rhizosphere microbiota.

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표/그림 (8)

그림 Fig. 1. Location of sampling site, A) Goraebul Coast located in the East Sea of the Korean Peninsula. B) Aerial view of sampling site. The aerial images were collected using Google Earth (version 7.3.3) and modified for this study. Sampling sites are highlighted with white line (0.80 km).
표 Table 1. Distribution of mixed vegetation rhizosphere bacteria
표 Table 2. Taxonomical identity of isolates and similarity with type strain databases
표 Table 2. Taxonomical identity of isolates and similarity with type strain databases (continued)
그림 Fig. 2. Phylogenetic tree based on 16S rDNA sequences showing the positions of rhizosphere marine bacteria and representatives of other species related taxa. The evolutionary history was inferred using the Neighbor-Joining method [58]. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches. The evolutionary distances were computed using the Kimura 2-parameter method [28] and are in the unit of the number of base substitutions per site. The rate variation among sites was modeled with a gamma distribution (shape parameter = 1). All positions with less than 95% site coverage were eliminated. Since there are too many isolates to implement phylogenetic tree with appropriate resolution, Phylogenetic tree were constructed by distinguishing the species belonging to genus Bacillus (A) and non-Bacillus species (B). Used outgroup sequences for constructing phylogenetic tree (A) was Paenibacillus polymyxa type strain NR037006 and for tree (B) was Aureobasidum pullulans type strain M55639. 1
그림 Fig. 2. Phylogenetic tree based on 16S rDNA sequences showing the positions of rhizosphere marine bacteria and representatives of other species related taxa. The evolutionary history was inferred using the Neighbor-Joining method [58]. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches. The evolutionary distances were computed using the Kimura 2-parameter method [28] and are in the unit of the number of base substitutions per site. The rate variation among sites was modeled with a gamma distribution (shape parameter = 1). All positions with less than 95% site coverage were eliminated. Since there are too many isolates to implement phylogenetic tree with appropriate resolution, Phylogenetic tree were constructed by distinguishing the species belonging to genus Bacillus (A) and non-Bacillus species (B). Used outgroup sequences for constructing phylogenetic tree (A) was Paenibacillus polymyxa type strain NR037006 and for tree (B) was Aureobasidum pullulans type strain M55639. 2
표 Table 3. Diversity of mixed vegetation rhizosphere bacteria
그림 Fig 3. Taxon ratio of rhizosphere isolates from mixed vegetation

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format