[논문]카무트밀 효소 식이가 Dextran Sulfate Sodium으로 유발한 대장염 마우스에 미치는 효과

길범주; 백채윤; 이준이; 황예슬; 최여진; 손주희; 유미애; 이동훈; 이동헌

doi:10.6116/kjh.2024.39.1.31

카무트밀 효소 식이가 Dextran Sulfate Sodium으로 유발한 대장염 마우스에 미치는 효과
Effects of Kamut Wheat Enzyme Diet on Dextran Sulfate Sodium-induced Colitis Mice 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.39 no.1, 2024년, pp.31 - 38

길범주 (가천대학교 한의과대학 본초학교실) , 백채윤 (가천대학교 한의과대학 본초학교실) , 이준이 (가천대학교 한의과대학 본초학교실) , 황예슬 (가천대학교 한의과대학 본초학교실) , 최여진 (가천대학교 한의과대학 본초학교실) , 손주희 (가천대학교 한의과대학 본초학교실) , 유미애 (주식회사 메디크로) , 이동훈 (주식회사 그레인온) , 이동헌 (가천대학교 한의과대학 본초학교실)

Abstract ▼ AI-Helper

Objectives : Ulcerative colitis is a chronic recurrent inflammatory disease of the gastrointestinal tract. However, there are some drawbacks to long-term drug therapy such as the risk of opportunistic infections. Recently, there was an increasing interest on the use of khorasan Kamut wheat because of their higher value of selenium and fiber than modern wheat. The present study was aimed to investigate the effect of Kamut brand wheat enzyme (Kamut WE) diet on colon health in dextran sulfate sodium (DSS)-induced colitis mice. Methods : Female C57BL/6J mice were divided into 6 groups. (1) normal (Water and AIN-93G diet), (2) control (1.25% DSS and AIN-93G diet), (3) Kamut WE (1.25% DSS and Kamut WE diet), (4) normal (Water and AIN-93G diet), (5) control (2.50% DSS and AIN-93G diet), (6) Kamut WE (2.50% DSS and Kamut WE diet). Dietary intake, body weight change, disease activity index (DAI), colon length and spleen weight were monitored. Results : Kamut WE group alleviated colitis symptom, including dietary intake loss, DAI (weight loss, loose stools, bleeding), colon length shortening and spleen swelling. Further, Kamut WE diets showed a significant effect against pathological damage by the increased colon length, decreased DAI and spleen weight in DSS 1.25% as well as DSS 2.50%. Conclusions : Our study provides evidence that Kamut WE diet increased colon length, decreased DAI and spleen weight in intestinal inflammation.

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표/그림 (7)

표 Table 1. Ingredient (g) of experimental diet used in this study.
그림 Figure 1. Overview of in vivo experimental design. A) Normal and DSS 1.25% groups. C57BL/6J mice were fed 1.25% DSS for 13 days followed by water for 3 days. B) Normal and DSS 2.50 % groups. C57BL/6J mice were fed 2.50% DSS for 7 days followed by water for 5 days. The number of mice was 8 per group.
표 Table 2. Scoring of the disease activity index (DAI)
그림 Figure 2. Diet intake per group from C57BL/6J mice. A) Normal and DSS 1.25% groups for 13 days. B) Normal and DSS 2.50% groups for 7 days. The number of mice was 8 per group; **p < 0.01 vs. control by one-way ANOVA.
그림 Figure 3. Body weight (A, B) and Disease activity index (DAI) score (C, D) of C57BL/6J mice. A) Normal and DSS 1.25% groups body weight (%) for 13 days. B) Normal and DSS 2.50% groups body weight(%) for 7 days. C) Normal and DSS 1.25% groups DAI score for 13 days. D) Normal and DSS 2.50% groups DAI score for 7 days. The number of mice was 8 per group; ^##p < 0.01 vs. normal, ^###p < 0.001 vs. normal, **p < 0.01 vs. control, and ***p < 0.001 vs. control by one-way ANOVA.
그림 Figure 4. Colon length with body weight rate of C57BL/6J mice. A) Normal and DSS 1.25% groups. B) Normal and DSS 2.50% groups. The number of mice was 8 per group; ^###p < 0.001 vs. normal, and *p < 0.05 vs. control by one-way ANOVA.
그림 Figure 5. Spleen with body weight rate of C57BL/6J mice. A) Normal and DSS 1.25% groups. B) Normal and DSS 2.50% groups. The number of mice was 8 per group; ^###p < 0.001 vs. normal, *p < 0.05 vs. control, and ^#p < 0.05 vs. normal by one-way ANOVA.

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