Krüssel, Jan-Steffen
(Reproductive Immunology Laboratory, Department of Gynecology and Obstetrics, Stanford University Medical Center, Palo Alto, CaliforniaUSA)
,
Behr, Barry
(Reproductive Immunology Laboratory, Department of Gynecology and Obstetrics, Stanford University Medical Center, Palo Alto, CaliforniaUSA)
,
Hirchenhain, Jens
(Department of Obstetrics and Gynecology, Heinrich-Heine-University Medical Center, Dü)
,
Wen, Yan
(sseldorf, Germany)
,
Milki, Amin A
(Reproductive Immunology Laboratory, Department of Gynecology and Obstetrics, Stanford University Medical Center, Palo Alto, CaliforniaUSA)
,
Cupisti, Susanne
(Reproductive Immunology Laboratory, Department of Gynecology and Obstetrics, Stanford University Medical Center, Palo Alto, CaliforniaUSA)
,
Bielfeld, Peter
(Department of Obstetrics and Gynecology, Heinrich-Heine-University Medical Center, Dü)
,
Polan, Mary Lake
(sseldorf, Germany)
AbstractObjective: To detect the expression of vascular endothelial growth factor (VEGF) mRNA and/or secretion of VEGF protein by human preimplantation embryos.Design: Human preimplantation embryos not suitable for uterine transfer were examined for β-actin and VEGF mRNA expression. Culture m...
AbstractObjective: To detect the expression of vascular endothelial growth factor (VEGF) mRNA and/or secretion of VEGF protein by human preimplantation embryos.Design: Human preimplantation embryos not suitable for uterine transfer were examined for β-actin and VEGF mRNA expression. Culture media from normally fertilized and developing preimplantation embryos were assessed for VEGF protein secretion.Setting: Clinics and academic research laboratories at the Departments of Obstetrics and Gynecology at the Stanford University, Palo Alto, California and the Heinrich-Heine-University, Düsseldorf, Germany.Patient(s): Couples undergoing IVF by intracytoplasmic sperm injection for various reasons.Intervention(s): Six unfertilized oocytes and 33 pathologically fertilized (tripronucleic, 3PN) preimplantation embryos were examined for VEGF mRNA expression, and 16 embryos were examined for VEGF protein secretion.Main Outcome Measure(s): Embryonic expression of VEGF mRNA and VEGF protein as determined by reverse transcription (RT)/nested polymerase chain reaction (PCR) and ELISA.Result(s): VEGF mRNA and protein could not be detected in unfertilized oocytes. However, 30/33 preimplantation embryos did express VEGF mRNA (11/12 10-to-16–cell embryos, 3/4 morulae, 11/12 early blastocysts, 5/5 hatched blastocysts). The VEGF protein level was below the sensitivity of the ELISA.Conclusion(s): Production of VEGF may give the embryo the ability to induce neoangiogenesis at the implantation site, thus creating an environment necessary for its survival.
AbstractObjective: To detect the expression of vascular endothelial growth factor (VEGF) mRNA and/or secretion of VEGF protein by human preimplantation embryos.Design: Human preimplantation embryos not suitable for uterine transfer were examined for β-actin and VEGF mRNA expression. Culture media from normally fertilized and developing preimplantation embryos were assessed for VEGF protein secretion.Setting: Clinics and academic research laboratories at the Departments of Obstetrics and Gynecology at the Stanford University, Palo Alto, California and the Heinrich-Heine-University, Düsseldorf, Germany.Patient(s): Couples undergoing IVF by intracytoplasmic sperm injection for various reasons.Intervention(s): Six unfertilized oocytes and 33 pathologically fertilized (tripronucleic, 3PN) preimplantation embryos were examined for VEGF mRNA expression, and 16 embryos were examined for VEGF protein secretion.Main Outcome Measure(s): Embryonic expression of VEGF mRNA and VEGF protein as determined by reverse transcription (RT)/nested polymerase chain reaction (PCR) and ELISA.Result(s): VEGF mRNA and protein could not be detected in unfertilized oocytes. However, 30/33 preimplantation embryos did express VEGF mRNA (11/12 10-to-16–cell embryos, 3/4 morulae, 11/12 early blastocysts, 5/5 hatched blastocysts). The VEGF protein level was below the sensitivity of the ELISA.Conclusion(s): Production of VEGF may give the embryo the ability to induce neoangiogenesis at the implantation site, thus creating an environment necessary for its survival.
Development Coffin 102 735 1988 10.1242/dev.102.4.735 Embryonic vascular development, immunohistochemical identification of the origin and subsequent morphogenesis of the major vessel primordia in quail embryos
Science Keck 246 1309 1989 10.1126/science.2479987 Vascular permeability factor, an endothelial mitogen related to PDGF
Biol Chem Connolly 264 20017 1989 10.1016/S0021-9258(19)47212-8 Human vascular permeability factor. Isolation from U937 cells
Biochem Biophys Res Commun Ferrara 161 851 1989 10.1016/0006-291X(89)92678-8 Pituitary follicular cells secrete a new heparin-binding growth factor specific for vascular endothelial cells
Biol Reprod Charnock-Jones 48 1120 1993 10.1095/biolreprod48.5.1120 Identification and localization of alternately spliced mRNAs for vascular endothelial growth factor in human uterus and estrogen regulation in endometrial carcinoma cell lines
Adv Cancer Res Shibuya 67 281 1995 10.1016/S0065-230X(08)60716-2 Role of VEGF-Flt receptor system in normal and tumor angiogenesis
J Biol Chem Poltorak 272 7151 1997 10.1074/jbc.272.11.7151 VEGF145, a secreted vascular endothelial growth factor isoform that binds to extracellular matrix
Mol Endocrinol Houck 5 1806 1991 10.1210/mend-5-12-1806 The vascular endothelial growth factor family. Identification of a fourth molecular species and characterization of alternative splicing of RNA
Cancer Metast Rev Neufeld 15 153 1996 10.1007/BF00437467 Similarities and differences between the vascular endothelial growth factor (VEGF) splice variants
FASEB J Neufeld 13 9 1999 10.1096/fasebj.13.1.9 Vascular endothelial growth factor (VEGF) and its receptors
Nature Carmeliet 380 435 1996 10.1038/380435a0 Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele
Nature Ferrara 380 439 1996 10.1038/380439a0 Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene
J Clin Invest Shweiki 91 2235 1993 10.1172/JCI116450 Patterns of expression of vascular endothelial growth factor (VEGF) and VEGF receptors in mice suggests a role in hormonally regulated angiogenesis
Endocrinology Jakeman 133 848 1993 10.1210/endo.133.2.7688292 Developmental expression of binding sites and messenger ribonucleic acid for vascular endothelial growth factor suggests a role for this protein in vasculogenesis and angiogenesis
J Clin Endocrinol Metabol Gordon 81 353 1996 Vascular endothelial growth factor localization in human ovary and fallopian tubes
J Clin Endocrinol Metabol Shifren 81 3112 1996 Ovarian steroid regulation of vascular endothelial growth factor in the human endometrium
Mol Hum Reprod Krüssel 5 452 1999 10.1093/molehr/5.5.452 Expression of vascular endothelial growth factor (VEGF) transmembraneous receptors Flt1 and KDR, and the soluble receptor sflt in cycling human endometrium
Fertil Steril Milki 72 225 1999 10.1016/S0015-0282(99)00262-9 Two-blastocyst transfer has similar pregnancy rates and a decreased multiple gestation rate compared with three-blastocyst transfer
J Reprod Immunol Krüssel 34 103 1997 10.1016/S0165-0378(97)00030-2 Different patterns of interleukin-1β (IL-1β), interleukin-1 receptor antagonist (IL-1ra) and interleukin-1 receptor type I (IL-1R tI) mRNA-expression in single preimplantation mouse embryos at various developmental stages
Hum Reprod Krüssel 13 2206 1998 10.1093/humrep/13.8.2206 Expression of interleukin-1 (IL-1) system mRNAs in single blastomeres from human preimplantation embryos
J Clin Endocrinol Metab Krüssel 83 953 1998 Single blastomeres within human preimplantation embryos express different amounts of messenger ribonucleic acid for β-actin and interleukin-1 receptor type I
J Reprod Fertil Suppl Krüssel 55 147 2000 Is there a place for biochemical embryonic preimplantational screening?
Hum Reprod Huang 12 1537 1997 10.1093/humrep/12.7.1537 Use of reverse transcription/polymerase chain reaction to detect embryonic interleukin-1 system messenger RNAs in individual preimplantation mouse embryos cocultured with Vero cells
BioTechniques Kumazaki 16 1017 1994 Detection of mRNA expression in a single cell by direct RT-PCR
Nucleic Acids Res Ponte 12 1687 1984 10.1093/nar/12.3.1687 Evolutionary conservation in the untranslated regions of actin mRNAs
Science Leung 246 1306 1989 10.1126/science.2479986 Vascular endothelial growth factor is a secreted angiogenic mitogen
Fertil Steril Alves da Motta 70 659 1998 10.1016/S0015-0282(98)00263-5 High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media
Hum Reprod Gardner 13 3434 1998 10.1093/humrep/13.12.3434 A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization
N Engl J Med Callahan 331 244 1994 10.1056/NEJM199407283310407 The economic impact of multiple-gestation pregnancies and the contribution of assisted-reproduction techniques to their incidence
Gynäkologe Bielfeld 31 203 1998 10.1007/PL00003113 Contribution of assisted reproductive techniques to the incidence of multiple gestation pregnancies
Fertil Steril Gardner 69 84 1998 10.1016/S0015-0282(97)00438-X Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers
Semin Reprod Endocrinol Tazuke 14 231 1996 10.1055/s-2007-1016333 Growth factors and cytokines in endometrium, embryonic development, and maternal:embryonic interactions
10.1007/978-1-4615-3380-1_8 Wegmann TG. The role of lymphohematopoietic cytokines in signaling between the immune and reproductive systems. In: Strauss III JF, Lyttle CR, eds. Uterine and embryonic factors in early pregnancy. New York: Plenum Press, 1991:87-96.
Genes Dev Rothstein 6 1190 1992 10.1101/gad.6.7.1190 Gene expression during preimplantation mouse development
Biol Reprod Sharkey 53 974 1995 10.1095/biolreprod53.4.974 Stage-specific expression of cytokine and receptor messenger ribonucleic acids in human preimplantation embryos
Nature Braude 332 459 1988 10.1038/332459a0 Human gene expression first occurs between the four- and eight-cell stages of preimplantation development
J Reprod Fertil Tesarik 78 463 1986 10.1530/jrf.0.0780463 Activation of nucleolar and extranucleolar RNA synthesis and changes in the ribosomal content of human embryos developing in vitro
Fertil Steril Van Blerkom 41 686 1984 10.1016/S0015-0282(16)47833-7 Preimplantation human embryonic development from polypronuclear eggs after in vitro fertilization
Ann Hum Genet Jacobs 46 223 1982 10.1111/j.1469-1809.1982.tb00714.x Human triploidy
Obstet Gynecol Werteleki 47 69 1976 The clinical syndrome of triploidy
Trounson 227 1982 Human conception in vitro Risks of triploidy arising through polyspermy in vitro
이 논문을 인용한 문헌
활용도 분석정보
상세보기
다운로드
내보내기
활용도 Top5 논문
해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다. 더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.