[논문]Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT

doi:10.1016/S0168-0102(00)00188-7

[해외논문] Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT

Neuroscience research : the official journal of the Japan Neuroscience Society, v.38 no.4, 2000년, pp.325 - 329

Abe, Kazuho (Corresponding author. Present address: Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan. Tel.: +81-3-54985785) , Matsuki, Norio (fax: +81-3-54985787)

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Abstract3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay and lactate dehydrogenase (LDH) release assay have been widely used for evaluating cell viability in culture. MTT reduction assay measures the redox activity of living cells, while LDH assay measures the activity of LDH released into the medium from dead cells. In this paper, we introduce a quick and simple method of measuring cellular MTT reduction and LDH release with the same dye, MTT. The substrate mixture for measuring LDH activity contained lactate, β-nicotinamide adenine dinucleotide, 1-methoxyphenazine methosulfate, MTT and Triton X-100. When the medium containing LDH was mixed with the substrates, MTT was converted into MTT formazan in proportion to LDH activity. This method was successfully applied for evaluating t-butyl hydroperoxide toxicity in cultured rat cortical astrocytes and glutamate toxicity in cultured rat hippocampal neurons. Our method is economical and convenient especially for measuring cellular MTT reduction and LDH release in the same culture.

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J. Neurochem. Abe 67 2074 1996 10.1046/j.1471-4159.1996.67052074.x Amyloid β toxicity consists of a Ca2+-independent early phase and a Ca2+-dependent late phase

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Jpn. J. Pharmacol. Abe 72 299 1996 10.1254/jjp.72.299 Menadione toxicity in cultured rat cortical astrocytes

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Pharmacol. Toxicol. Abe 83 40 1998 10.1111/j.1600-0773.1998.tb01440.x Characterization of t-butyl hydroperoxide toxicity in cultured rat cortical neurons and astrocytes

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J. Immunol. Methods Decker 115 61 1988 10.1016/0022-1759(88)90310-9 A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity

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J. Immunol. Methods Hansen 119 203 1989 10.1016/0022-1759(89)90397-9 Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell killing

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J. Biochem. Hisada 82 1469 1977 10.1093/oxfordjournals.jbchem.a131836 1-Methoxy-5-methylphenazinium methyl sulfate: a photochemically stable electron mediator between NADH and various electron acceptors

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J. Immunol. Methods Korzeniewski 64 313 1983 10.1016/0022-1759(83)90438-6 An enzyme-release assay for natural cytotoxicity

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Arch. Biochem. Biophys. Masaki 269 390 1989 10.1016/0003-9861(89)90122-7 tert-Butyl hydroperoxide kills cultured hepatocytes by peroxidizing membrane lipids

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[해외논문] Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT

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[해외논문] Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT

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