The recent increase in elderly population has contributed to the increasing interest in the foods for the elderly. This research focuses on softening the texture of job’s-tear granules by enzyme treatment and the evaluation of their constipation improvement function via in vivo evaluation usi...
The recent increase in elderly population has contributed to the increasing interest in the foods for the elderly. This research focuses on softening the texture of job’s-tear granules by enzyme treatment and the evaluation of their constipation improvement function via in vivo evaluation using mouse models. After enzyme treatment, the Job’s-tears exhibited a marked softening with a 70% reduction of hardness upon alternating treatment with commercial amylase and protease for 2 hours at 0.1% concentrations. Microstructure analysis with scanning electron microscopy revealed the degree of collapse of the Job’s-tear granules and the level of tissue degradation. Constipation was induced using loperamide, and the relief effect of softened Job’s-tears was determined over three dosage (500, 1,000, 2,000 mg/kg) using the data from the number of feces, and blood tests in the mouse model. The optimum dose of the enzymatized Job’s-tears was determined to be 500 mg/kg (EA500), which effectively improve excretion movement without liver toxicity. Compared with the control group, oral administration of enzymatized Job’s-tear resulted in higher fecal moisture content, higher fecal numbers, and increased length and width of intestinal epithelial cells in the mucous membranes. In conclusion, softened Job’s-tears were successfully produced by enzyme treatment, and their constipation relief function was confirmed in a mouse model. The results of the present investigation are expected to contribute to the development of foods for the elderly.
The recent increase in elderly population has contributed to the increasing interest in the foods for the elderly. This research focuses on softening the texture of job’s-tear granules by enzyme treatment and the evaluation of their constipation improvement function via in vivo evaluation using mouse models. After enzyme treatment, the Job’s-tears exhibited a marked softening with a 70% reduction of hardness upon alternating treatment with commercial amylase and protease for 2 hours at 0.1% concentrations. Microstructure analysis with scanning electron microscopy revealed the degree of collapse of the Job’s-tear granules and the level of tissue degradation. Constipation was induced using loperamide, and the relief effect of softened Job’s-tears was determined over three dosage (500, 1,000, 2,000 mg/kg) using the data from the number of feces, and blood tests in the mouse model. The optimum dose of the enzymatized Job’s-tears was determined to be 500 mg/kg (EA500), which effectively improve excretion movement without liver toxicity. Compared with the control group, oral administration of enzymatized Job’s-tear resulted in higher fecal moisture content, higher fecal numbers, and increased length and width of intestinal epithelial cells in the mucous membranes. In conclusion, softened Job’s-tears were successfully produced by enzyme treatment, and their constipation relief function was confirmed in a mouse model. The results of the present investigation are expected to contribute to the development of foods for the elderly.
참고문헌 (21)
Korean Statistical Information Service: http://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1BPA002&vw_cd=&list_id=&scrId=&seqNo=&lang_mode=ko&obj_var_id=&itm_id=&conn_path=E1. accessed July, 2019
National Health Statistics: https://knhanes.cdc.go.kr/knhanes/sub04/sub04_03.do?classType=7. accessed July, 2019
Kim KH (2014) Optimization for enzyme treatments on the texture and digestibility of vegetable foods. MS Thesis, Chung-Ang University, Korea, p 4-36
Lee HJ (2016) Development of impregnation technology for vitamin C fortification and tenderization of foods. MS Thesis, CHA University, Korea, p 1-59
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