[논문]Super-Resolution Three-Dimensional Imaging of Actin Filaments in Cultured Cells and the Brain via Expansion Microscopy

Park, Chan E; Cho, Youngbin; Cho, In; Jung, Hyunsu; Kim, Byeongyeon; Shin, Jennifer H.; Choi, Sungyoung; Kwon, Seok-Kyu; Hahn, Young Ki; Chang, Jae-Byum

doi:10.1021/acsnano.0c04915

[해외논문] Super-Resolution Three-Dimensional Imaging of Actin Filaments in Cultured Cells and the Brain via Expansion Microscopy

ACS nano, v.14 no.11, 2020년, pp.14999 - 15010

Park, Chan E (Department of Materials Science and Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Korea) , Cho, Youngbin (Department of Mechanical Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Korea) , Cho, In (Department of Materials Science and Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Korea) , Jung, Hyunsu (Division of Life Sciences , Korea University , Seoul 02841 , Korea) , Kim, Byeongyeon (Department of Electronic Engineering , Hanyang University , Seoul 04763 , Korea) , Shin, Jennifer H. (Department of Mechanical Engineering , Korea Advanced Institute of Science and Technology , Daejeon 34141 , Korea) , Choi, Sungyoung (Department of Electronic Engineering , Hanyang University , Seoul 04763 , Korea) , Kwon, Seok-Kyu , Hahn, Young Ki , Chang, Jae-Byum

Abstract ▼ AI-Helper

Actin is an essential protein in almost all life forms. It mediates diverse biological functions, ranging from controlling the shape of cells and cell movements to cargo transport and the formation of synaptic connections. Multiple diseases are closely related to the dysfunction of actin or actin-related proteins. Despite the biological importance of actin, super-resolution imaging of it in tissue is still challenging, as it forms very dense networks in almost all cells inside the tissue. In this work, we demonstrate multiplexed super-resolution volumetric imaging of actin in both cultured cells and mouse brain slices via expansion microscopy (ExM). By introducing a simple labeling process, which enables the anchoring of an actin probe, phalloidin, to a swellable hydrogel, the multiplexed ExM imaging of actin filaments was achieved. We first showed that this technique could visualize the nanoscale details of actin filament organizations in cultured cells. Then, we applied this technique to mouse brain slices and visualized diverse actin organizations, such as the parallel actin filaments along the long axis of dendrites and dense actin structures in postsynaptic spines. We examined the postsynaptic spines in the mouse brain and showed that the organizations of actin filaments are highly diverse. This technique, which enables the high-throughput 60 nm resolution imaging of actin filaments and other proteins in cultured cells and thick tissue slices, would be a useful tool to study the organization of actin filaments in diverse biological circumstances and how they change under pathological conditions.[FIG OMISSION]

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