[논문]A real-time PCR method to assess the presence of vertebrate material in plant-based products

Cottenet, Geoffrey; Blancpain, Carine

doi:10.1016/j.foodcont.2021.108001

[해외논문] A real-time PCR method to assess the presence of vertebrate material in plant-based products

Food control, v.125, 2021년, pp.108001 -

Cottenet, Geoffrey (Corresponding author.) , Blancpain, Carine

Abstract ▼ AI-Helper

Abstract Consumption of plant-based products and meat alternatives has been rising for a few years, with vegetarian and vegan diets gaining more and more popularity. To comply with legislation and maintain consumers’ trust, food manufacturers of vegan products must ensure the absence of animal residues in these products. A real-time PCR method targeting the 16S mitochondrial rRNA gene from vertebrates has been developed in order to detect the presence of vertebrate material in plant-based ingredients and finished products. The reaction successfully amplified on mammals, birds, reptiles and fish samples only. The analysis of plant-based samples spiked at 0.1% (w/w) with meat or fish materials confirmed the sensitivity of the method at this level, considered as the limit for unintended presence of animal residues by the European Vegetarian Union. The method was applied to commercialized vegan finished products from European markets and confirmed the absence of animal material in all the tested samples. Highlights New real-time PCR method to detect vertebrates in plant-based products. Limit of detection determined at 0.1% (w/w) aligned with the European V-label. Method applicable on plant-based ingredients and vegan finished products. 24 commercialized European vegan products tested, none contained animal material.

주제어

참고문헌 (22)

Meat Science Ballin 83 165 2009 10.1016/j.meatsci.2009.06.003 Species determination - can we detect and quantify meat adulteration?

상세보기
2019 Vegan sues burger king for cooking impossible whopper on meat grill
BOLDsystems. Barcode of life data system. http://www.boldsystems.org/Accessed 22.11.2020.
Catalogue of Life. Species 2000 & ITIS catalogue of life, 2020-02-24. http://www.catalogueoflife.org/col/Accessed 01.04.2020.
Cavin 670 2019 Encyclopedia of food chemistry Food fraud vulnerabilities in the supply chain: An industry perspective
Journal of Food Protection Cawthraw 72 1055 2009 10.4315/0362-028X-72.5.1055 Real-time PCR detection and identification of prohibited mammalian and avian material in animal feeds

상세보기
Food Control Cottenet 110 2020 10.1016/j.foodcont.2019.107003 Evaluation and application of a next generation sequencing approach for meat species identification

상세보기
Food Control Cottenet 67 135 2016 10.1016/j.foodcont.2016.02.042 A DNA macro-array to simultaneously identify 32 meat species in food samples

상세보기
European Vegetarian Union. Criteria of the V-label: Regulations. https://www.v-label.eu/regulations_text Accessed 20.03.2020.
Food Chemistry Floren 173 1054 2015 10.1016/j.foodchem.2014.10.138 Species identification and quantification in meat and meat products using droplet digital PCR (ddPCR)

상세보기
2019 Meat substitutes market report FBI100239
Gray 1 2012 Genomics of chloroplasts and mitochondria Origins of mitochondria and plastids
Food Additives & Contaminants Hird 23 645 2006 10.1080/02652030600603041 Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction

상세보기
Critical Reviews in Food Science and Nutrition Kumar 10 1340 2015 10.1080/10408398.2012.693978 Identification of species origin of meat and meat products on the DNA basis: A review

상세보기
BMC Genetics Nadjar-Boger 12 22 2011 10.1186/1471-2156-12-22 Myostatin-2 gene structure and polymorphsim of the promoter and first intron in the marine fish Sparus aurata: Evidence for DNA duplications and/or translocations

상세보기
2013 National library of medicine (US)
Food Control Ren 76 42 2017 10.1016/j.foodcont.2017.01.003 A novel quantitative real-time PCR method for identification and quantification of mammalian and poultry species in foods

상세보기
2020 Plant-based protein - global market outlook (2018-2027) ID 4897509
Analytical and Bioanalytical Chemistry Staats 408 4615 2016 10.1007/s00216-016-9595-8 Advances in DNA metabarcoding for food and wildlife forensic species identification

상세보기
European Food Research and Technology Tetzlaff 243 849 2017 10.1007/s00217-016-2799-5 Development of a real-time PCR system for the detection of the potential allergen fish in food

상세보기
2018 Supermarket scandal: Pork and Turkey found in vegan and 'meat free' meals
Vegan Society. Statistics. https://www.vegansociety.com/news/media/statistics Accessed 20.03.2020.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[해외논문] A real-time PCR method to assess the presence of vertebrate material in plant-based products

Abstract ▼ AI-Helper

주제어

참고문헌 (22)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

연관된 기능

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트