[논문]Cordyceps militaris induces apoptosis in ovarian cancer cells through TNF-α/TNFR1-mediated inhibition of NF-κB phosphorylation

Jo, Eunbi; Jang, Hyun-Jin; Yang, Kyeong Eun; Jang, Min Su; Huh, Yang Hoon; Yoo, Hwa-Seung; Park, Jun Soo; Jang, Ik-Soon; Park, Soo Jung

doi:10.1186/s12906-019-2780-5

[해외논문] Cordyceps militaris induces apoptosis in ovarian cancer cells through TNF-α/TNFR1-mediated inhibition of NF-κB phosphorylation 원문보기

Bmc complementary medicine and therapies, v.20 no.1, 2020년, pp.1 -

Jo, Eunbi (Division of Analytical Science, Korea Basic Science Institute, Gwahangno 113, Yuseong-gu, Daejeon, 305-333 Republic of Korea) , Jang, Hyun-Jin (Division of Analytical Science, Korea Basic Science Institute, Gwahangno 113, Yuseong-gu, Daejeon, 305-333 Republic of Korea) , Yang, Kyeong Eun (Division of Analytical Science, Korea Basic Science Institute, Gwahangno 113, Yuseong-gu, Daejeon, 305-333 Republic of Korea) , Jang, Min Su (Division of Biological Science and Technology, Yonsei University, Wonju, 220-100 Republic of Korea) , Huh, Yang Hoon (Electron Microscopy Research Center, Korea Basic Science Institute, Cheongju, 28119 Republic of Korea) , Yoo, Hwa-Seung (East-West Cancer Center, Daejeon University, Daejeon, 302-120 South Korea) , Park, Jun Soo (Division of Biological Science and Technology, Yonsei University, Wonju, 220-100 Republic of Korea) , Jang, Ik-Soon (Division of Analytical Science, Korea Basic Science Institute, Gwahangno 113, Yuseong-gu, Daejeon, 305-333 Republic of Korea) , Park, Soo Jung (Department of Sasang Constitutional Medicine, College of Korean Medicine, Woosuk University, Wanju, Jeonbuk 55)

Abstract ▼ AI-Helper

BackgroundCordyceps militaris (L.) Fr. (C. militaris) exhibits pharmacological activities, including antitumor properties, through the regulation of the nuclear factor kappa B (NF-κB) signaling. Tumor Necrosis Factor (TNF) and TNF-α modulates cell survival and apoptosis through NF- κB signaling. However, the mechanism underlying its mode of action on the NF-κB pathway is unclear.MethodsHere, we analyzed the effect of C. militaris extract (CME) on the proliferation of ovarian cancer cells by confirming viability, morphological changes, migration assay. Additionally, CME induced apoptosis was determined by apoptosis assay and apoptotic body formation under TEM. The mechanisms of CME were determined through microarray, immunoblotting and immunocytochemistry.ResultsCME reduced the viability of cells in a dose-dependent manner and induced morphological changes. We confirmed the decrease in the migration activity of SKOV-3 cells after treatment with CME and the consequent induction of apoptosis. Immunoblotting results showed that the CME-mediated upregulation of tumor necrosis factor receptor 1 (TNFR1) expression induced apoptosis of SKOV-3 cells via the serial activation of caspases. Moreover, CME negatively modulated NF-κB activation via TNFR expression, suggestive of the activation of the extrinsic apoptotic pathway. The binding of TNF-α to TNFR results in the disassociation of IκB from NF-κB and the subsequent translocation of the active NF-κB to the nucleus. CME clearly suppressed NF-κB translocation induced by interleukin (IL-1β) from the cytosol into the nucleus. The decrease in the expression levels of B cell lymphoma (Bcl)-xL and Bcl-2 led to a marked increase in cell apoptosis.ConclusionThese results suggest that C. militaris inhibited ovarian cancer cell proliferation, survival, and migration, possibly through the coordination between TNF-α/TNFR1 signaling and NF-κB activation. Taken together, our findings provide a new insight into a novel treatment strategy for ovarian cancer using C. militaris.

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참고문헌 (55)

1. Vaughan S Coward JI Bast RC Jr Berchuck A Berek JS Brenton JD Coukos G Crum CC Drapkin R Etemadmoghadam D Rethinking ovarian cancer: recommendations for improving outcomes Nat Rev Cancer 2011 11 10 719 725 10.1038/nrc3144 21941283

상세보기
2. Klymenko Yuliya Nephew Kenneth P. Epigenetic Crosstalk between the Tumor Microenvironment and Ovarian Cancer Cells: A Therapeutic Road Less Traveled Cancers 2018 10 9 295 10.3390/cancers10090295 6162502

상세보기
3. Boyd LR Muggia FM Carboplatin/paclitaxel induction in ovarian Cancer: the finer points Oncol (Williston Park) 2018 32 8 418？420 422-414
4. Cavaliere F, Giannarelli D, Valle M, Federici O, Liotta G, Perri P, Di Filippo F, Garofalo A, Ialongo P: Peritoneal carcinomatosis from ovarian epithelial primary: combined aggressive treatment. In Vivo 2009, 23(3):441？446.
5. Pan SY Litscher G Gao SH Zhou SF Yu ZL Chen HQ Zhang SF Tang MK Sun JN Ko KM Historical perspective of traditional indigenous medical practices: the current renaissance and conservation of herbal resources Evid Based Complement Alternat Med 2014 2014 525340 24872833

상세보기
6. Ji XM Wu ZC Liu GW Yu HY Liu H Wang ZT Wei XH Ouyang B Wenxia Changfu formula () induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice Chin J Integr Med 2016 22 10 752 758 10.1007/s11655-015-2087-4 26666762

상세보기
7. Siu KM Mak DH Chiu PY Poon MK Du Y Ko KM Pharmacological basis of 'Yin-nourishing' and 'Yang-invigorating' actions of Cordyceps, a Chinese tonifying herb Life Sci 2004 76 4 385 395 10.1016/j.lfs.2004.07.014 15530501

상세보기
8. Ng TB Wang HX Pharmacological actions of Cordyceps, a prized folk medicine J Pharm Pharmacol 2005 57 12 1509 1519 10.1211/jpp.57.12.0001 16354395

상세보기
9. Yue K Ye M Zhou Z Sun W Lin X The genus Cordyceps: a chemical and pharmacological review J Pharm Pharmacol 2013 65 4 474 493 10.1111/j.2042-7158.2012.01601.x 23488776

상세보기
10. Park C Hong SH Lee JY Kim GY Choi BT Lee YT Park DI Park YM Jeong YK Choi YH Growth inhibition of U937 leukemia cells by aqueous extract of Cordyceps militaris through induction of apoptosis Oncol Rep 2005 13 6 1211 1216 15870944
11. Lee H Kim YJ Kim HW Lee DH Sung MK Park T Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells Biol Pharm Bull 2006 29 4 670 674 10.1248/bpb.29.670 16595897

상세보기
12. Rao YK Fang SH Wu WS Tzeng YM Constituents isolated from Cordyceps militaris suppress enhanced inflammatory mediator's production and human cancer cell proliferation J Ethnopharmacol 2010 131 2 363 367 10.1016/j.jep.2010.07.020 20633630

상세보기
13. Sung NY Kim SC Kim YH Kim G Lee Y Sung GH Kim JH Yang WS Kim MS Baek KS Anti-proliferative and pro-apoptotic activities of 4-Methyl-2,6-bis(1-phenylethyl) phenol in Cancer cells Biomol Ther (Seoul) 2016 24 4 402 409 10.4062/biomolther.2015.166 27068261

원문보기 상세보기
14. Bizarro A Ferreira IC Sokovic M van Griensven LJ Sousa D Vasconcelos MH Lima RT Cordyceps militaris (L.) link fruiting body reduces the growth of a non-small cell lung Cancer cell line by increasing cellular levels of p53 and p21 Mol 2015 20 8 13927 13940 10.3390/molecules200813927

상세보기
15. Lee HH Lee S Lee K Shin YS Kang H Cho H Anti-cancer effect of Cordyceps militaris in human colorectal carcinoma RKO cells via cell cycle arrest and mitochondrial apoptosis Daru 2015 23 35 10.1186/s40199-015-0117-6 26141646

상세보기
16. Xiao JH Zhong JJ Secondary metabolites from Cordyceps species and their antitumor activity studies Recent Pat Biotechnol 2007 1 2 123 137 10.2174/187220807780809454 19075836
17. Hwang JH Joo JC Kim DJ Jo E Yoo HS Lee KB Park SJ Jang IS Cordycepin promotes apoptosis by modulating the ERK-JNK signaling pathway via DUSP5 in renal cancer cells Am J Cancer Res 2016 6 8 1758 1771 27648363

상세보기
18. Hwang JH Park SJ Ko WG Kang SM Lee DB Bang J Park BJ Wee CB Kim DJ Jang IS Cordycepin induces human lung cancer cell apoptosis by inhibiting nitric oxide mediated ERK/slug signaling pathway Am J Cancer Res 2017 7 3 417 432 28401001

상세보기
19. Hwang IH Oh SY Jang HJ Jo E Joo JC Lee KB Yoo HS Lee MY Park SJ Jang IS Cordycepin promotes apoptosis in renal carcinoma cells by activating the MKK7-JNK signaling pathway through inhibition of c-FLIPL expression PLoS One 2017 12 10 e0186489 10.1371/journal.pone.0186489 29045468

상세보기
20. Joo JC Hwang JH Jo E Kim YR Kim DJ Lee KB Park SJ Jang IS Cordycepin induces apoptosis by caveolin-1-mediated JNK regulation of Foxo3a in human lung adenocarcinoma Oncotarget 2017 8 7 12211 12224 10.18632/oncotarget.14661 28099944

상세보기
21. Cui ZY Park SJ Jo E Hwang IH Lee KB Kim SW Kim DJ Joo JC Hong SH Lee MG Cordycepin induces apoptosis of human ovarian cancer cells by inhibiting CCL5-mediated Akt/NF-kappaB signaling pathway Cell Death Discov 2018 4 62 10.1038/s41420-018-0063-4 29844932

상세보기
22. Locksley RM Killeen N Lenardo MJ The TNF and TNF receptor superfamilies: integrating mammalian biology Cell 2001 104 4 487 501 10.1016/S0092-8674(01)00237-9 11239407

상세보기
23. Dempsey PW Doyle SE He JQ Cheng G The signaling adaptors and pathways activated by TNF superfamily Cytokine Growth Factor Rev 2003 14 3？4 193 209 10.1016/S1359-6101(03)00021-2 12787559

상세보기
24. Micheau O Tschopp J Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes Cell 2003 114 2 181 190 10.1016/S0092-8674(03)00521-X 12887920

상세보기
25. Huycke MM Abrams V Moore DR Enterococcus faecalis produces extracellular superoxide and hydrogen peroxide that damages colonic epithelial cell DNA Carcinogenesis 2002 23 3 529 536 10.1093/carcin/23.3.529 11895869

상세보기
26. Wang X Huycke MM Extracellular superoxide production by enterococcus faecalis promotes chromosomal instability in mammalian cells Gastroenterol 2007 132 2 551 561 10.1053/j.gastro.2006.11.040

상세보기
27. Balkwill F Tumour necrosis factor and cancer Nat Rev Cancer 2009 9 5 361 371 10.1038/nrc2628 19343034

상세보기
28. Thommesen L Laegreid A Distinct differences between TNF receptor 1- and TNF receptor 2-mediated activation of NFkappaB J Biochem Mol Biol 2005 38 3 281 289 15943902
29. Terzic J Grivennikov S Karin E Karin M Inflammation and colon cancer Gastroenterol 2010 138 6 2101 2114 10.1053/j.gastro.2010.01.058

상세보기
30. Ben-Neriah Y Karin M Inflammation meets cancer, with NF-kappaB as the matchmaker Nat Immunol 2011 12 8 715 723 10.1038/ni.2060 21772280

상세보기
31. Popivanova BK Kitamura K Wu Y Kondo T Kagaya T Kaneko S Oshima M Fujii C Mukaida N Blocking TNF-alpha in mice reduces colorectal carcinogenesis associated with chronic colitis J Clin Invest 2008 118 2 560 570 18219394

상세보기
32. Sedger LM McDermott MF TNF and TNF-receptors: from mediators of cell death and inflammation to therapeutic giants - past, present and future Cytokine Growth Factor Rev 2014 25 4 453 472 10.1016/j.cytogfr.2014.07.016 25169849

상세보기
33. Jupp OJ McFarlane SM Anderson HM Littlejohn AF Mohamed AA MacKay RH Vandenabeele P MacEwan DJ Type II tumour necrosis factor-alpha receptor (TNFR2) activates c-Jun N-terminal kinase (JNK) but not mitogen-activated protein kinase (MAPK) or p38 MAPK pathways Biochem J 2001 359 Pt 3 525 535 10.1042/bj3590525 11672426

상세보기
34. Yang Y Wang X Moore DR Lightfoot SA Huycke MM TNF-alpha mediates macrophage-induced bystander effects through Netrin-1 Cancer Res 2012 72 20 5219 5229 10.1158/0008-5472.CAN-12-1463 22915753

상세보기
35. Wolf Y Shemer A Polonsky M Gross M Mildner A Yona S David E Kim KW Goldmann T Amit I Autonomous TNF is critical for in vivo monocyte survival in steady state and inflammation J Exp Med 2017 214 4 905 917 10.1084/jem.20160499 28330904

상세보기
36. Martinez-Reza I Diaz L Garcia-Becerra R Preclinical and clinical aspects of TNF-alpha and its receptors TNFR1 and TNFR2 in breast cancer J Biomed Sci 2017 24 1 90 10.1186/s12929-017-0398-9 29202842

상세보기
37. Soo Jung Park H-JJ Hwang I-H Kim J m Jo E Lee m-g Jang I-s Joo JC Cordyceps militaris extract inhibits the NF-κB pathway and induces apoptosis through MKK7-JNK signaling activation in TK-10 human renal cell carcinoma Nat Prod Commun 2018 13 4 465 470
38. Cui ZY, Jo E, Jang HJ, Hwang IH, Lee KB, Yoo HS, Park SJ, Jung MK, Lee YW, Jang IS. Modified ginseng extract induces apoptosis in HepG2 Cancer cells by blocking the CXCL8-mediated Akt/nuclear factor-[formula: see text] B signaling pathway. Am J Chin Med. 2018:1？18.
39. Szlosarek PW Grimshaw MJ Kulbe H Wilson JL Wilbanks GD Burke F Balkwill FR Expression and regulation of tumor necrosis factor alpha in normal and malignant ovarian epithelium Mol Cancer Ther 2006 5 2 382 390 10.1158/1535-7163.MCT-05-0303 16505113

상세보기
40. Chen W Zheng R Baade PD Zhang S Zeng H Bray F Jemal A Yu XQ He J Cancer statistics in China, 2015 CA Cancer J Clin 2016 66 2 115 132 10.3322/caac.21338 26808342

상세보기
41. Marcus CS Maxwell GL Darcy KM Hamilton CA McGuire WP Current approaches and challenges in managing and monitoring treatment response in ovarian cancer J Cancer 2014 5 1 25 30 10.7150/jca.7810 24396495

상세보기
42. Coukos G Rubin SC Chemotherapy resistance in ovarian cancer: new molecular perspectives Obstet Gynecol 1998 91 5 Pt 1 783 792 9572231
43. Godwin P Baird AM Heavey S Barr MP O'Byrne KJ Gately K Targeting nuclear factor-kappa B to overcome resistance to chemotherapy Front Oncol 2013 3 120 10.3389/fonc.2013.00120 23720710

상세보기
44. Kartalou M Essigmann JM Mechanisms of resistance to cisplatin Mutat Res 2001 478 1？2 23 43 10.1016/S0027-5107(01)00141-5 11406167

상세보기
45. Thevenod F Friedmann JM Katsen AD Hauser IA Up-regulation of multidrug resistance P-glycoprotein via nuclear factor-kappaB activation protects kidney proximal tubule cells from cadmium- and reactive oxygen species-induced apoptosis J Biol Chem 2000 275 3 1887 1896 10.1074/jbc.275.3.1887 10636889

상세보기
46. Chuang SE Yeh PY Lu YS Lai GM Liao CM Gao M Cheng AL Basal levels and patterns of anticancer drug-induced activation of nuclear factor-kappaB (NF-kappaB), and its attenuation by tamoxifen, dexamethasone, and curcumin in carcinoma cells Biochem Pharmacol 2002 63 9 1709 1716 10.1016/S0006-2952(02)00931-0 12007574

상세보기
47. Kumar A Sunita P Jha S Pattanayak SP Daphnetin inhibits TNF-alpha and VEGF-induced angiogenesis through inhibition of the IKKs/IkappaBalpha/NF-kappaB, Src/FAK/ERK1/2 and Akt signalling pathways Clin Exp Pharmacol Physiol 2016 43 10 939 950 10.1111/1440-1681.12608 27297262

상세보기
48. Mabuchi S Ohmichi M Nishio Y Hayasaka T Kimura A Ohta T Saito M Kawagoe J Takahashi K Yada-Hashimoto N Inhibition of NFkappaB increases the efficacy of cisplatin in in vitro and in vivo ovarian cancer models J Biol Chem 2004 279 22 23477 23485 10.1074/jbc.M313709200 15026414

상세보기
49. Li Y Ahmed F Ali S Philip PA Kucuk O Sarkar FH Inactivation of nuclear factor kappaB by soy isoflavone genistein contributes to increased apoptosis induced by chemotherapeutic agents in human cancer cells Cancer Res 2005 65 15 6934 6942 10.1158/0008-5472.CAN-04-4604 16061678

상세보기
50. Tuli Hardeep S. Sandhu Sardul S. Sharma A. K. Pharmacological and therapeutic potential of Cordyceps with special reference to Cordycepin 3 Biotech 2013 4 1 1 12 10.1007/s13205-013-0121-9 28324458

상세보기
51. Park JG Son YJ Lee TH Baek NJ Yoon DH Kim TW Aravinthan A Hong S Kim JH Sung GH Anticancer efficacy of Cordyceps militaris ethanol extract in a Xenografted leukemia model Evid Based Complement Alternat Med 2017 2017 8474703 28761499

상세보기
52. Egan LJ Eckmann L Greten FR Chae S Li ZW Myhre GM Robine S Karin M Kagnoff MF IkappaB-kinasebeta-dependent NF-kappaB activation provides radioprotection to the intestinal epithelium Proc Natl Acad Sci U S A 2004 101 8 2452 2457 10.1073/pnas.0306734101 14983030

상세보기
53. Lu W Chen Q Ying S Xia X Yu Z Lui Y Tranter G Jin B Song C Seymour LW Evolutionarily conserved primary TNF sequences relate to its primitive functions in cell death induction J Cell Sci 2016 129 1 108 120 10.1242/jcs.175463 26729029

상세보기
54. Faustman D Davis M TNF receptor 2 pathway: drug target for autoimmune diseases Nat Rev Drug Discov 2010 9 6 482 493 10.1038/nrd3030 20489699

상세보기
55. Bremer E Targeting of the tumor necrosis factor receptor superfamily for cancer immunotherapy ISRN Oncol 2013 2013 371854 23840967

상세보기

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