[논문]Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

Roy, Pantu-Kumar; Qamar, Ahmad-Yar; Tanga, Bereket-Molla; Bang, Seonggyu; Seong, Gyeonghwan; Fang, Xun; Kim, Ghangyong; Edirisinghe, Shan-Lakmal; De Zoysa, Mahanama; Kang, Do-Hyung; Saadeldin, Islam M.; Cho, Jongki

doi:10.3390/ani11092483

[해외논문] Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes 원문보기

Animals an open access journal from MDPI, v.11 no.9, 2021년, pp.2483 -

Roy, Pantu-Kumar (College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea) , Qamar, Ahmad-Yar (vetpantu88@gmail.com (P.-K.R.)) , Tanga, Bereket-Molla (ahmad.qamar@uvas.edu.pk (A.-Y.Q.)) , Bang, Seonggyu (tanga@o.cnu.ac.kr (B.-M.T.)) , Seong, Gyeonghwan (bangsk97@o.cnu.ac.kr (S.B.)) , Fang, Xun (202050377@o.cnu.ac.kr (G.S.)) , Kim, Ghangyong (fx2442@o.cnu.ac.kr (X.F.)) , Edirisinghe, Shan-Lakmal (gykim1007@gmail.com (G.K.)) , De Zoysa, Mahanama (shan.lakmal09011@gmail.com (S.-L.E.)) , Kang, Do-Hyung (mahanama@cnu.ac.kr (M.D.Z.)) , Saadeldin, Islam M. (islamms@zu.edu.eg (I.M.S.)) , Cho, Jongki (College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea)

Abstract ▼ AI-Helper

Simple SummaryPoor in vitro embryo development is a major obstacle in porcine assisted reproduction. In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. Results showed that modified Spirulina maxima pectin nanoparticles at 2.5 µg/mL improved oocyte maturation in form of first polar body extrusion, reduced oxidative stress, and increased the developmental competence of the oocytes after parthenogenetic activation and somatic cell nuclear transfer. Moreover, the relative transcripts quantification showed significant increase in the pluripotency-associated transcripts in the resultant cloned embryos after modified Spirulina maxima pectin nanoparticles supplementation. Therefore, we provide an optimum in vitro maturation condition to improve the in vitro embryo production in porcine.AbstractMolecular approaches have been used to determine metabolic substrates involved in the early embryonic processes to provide adequate culture conditions. To investigate the effect of modified Spirulina maxima pectin nanoparticles (MSmPNPs) on oocyte developmental competence, cumulus–oocyte complexes (COCs) retrieved from pig slaughterhouse ovaries were subjected to various concentrations of MSmPNPs (0, 2.5, 5.0, and 10 µg/mL) during in vitro maturation (IVM). In comparison to the control, MSmPNPs-5.0, and MSmPNPs-10 groups, oocytes treated with 2.5 µg/mL MSmPNPs had significantly increased glutathione (GSH) levels and lower levels of reactive oxygen species (ROS). Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. Furthermore, similar findings were reported for the developmental competence of somatic cell nuclear transfer (SCNT)-derived embryos. Additionally, the relative quantification of POU5F1, DPPA2, and NDP52 mRNA transcript levels were significantly higher in the MSmPNPs-2.5 group than in the control and other treated groups. Taken together, the current findings suggest that MSmPNP treatment alleviates oxidative stress and enhances the developmental competence of porcine in vitro matured oocytes after parthenogenetic activation and SCNT.

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더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
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