[논문]A flavone from the ethyl acetate extract of Leea rubra leaves with DNA damage protection and antineoplastic activity

Das, Nibedita; Parvin, Mst. Shahnaj; Hasan, Mahadi; Akter, Masuma; Hossain, Md. Sanowar; Parvez, G.M.Masud; Sarker, Ashish Kumar; Abdur Rahman, Md. Aziz; Mamun, Al; Islam, Md. Ekramul

doi:10.1016/j.bbrep.2022.101244

A flavone from the ethyl acetate extract of Leea rubra leaves with DNA damage protection and antineoplastic activity 원문보기

Biochemistry and biophysics reports, v.30, 2022년, pp.101244 -

Das, Nibedita (Institute of Biological Science, University of Rajshahi, Rajshahi, 6205, Bangladesh) , Parvin, Mst. Shahnaj (Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh) , Hasan, Mahadi (Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh) , Akter, Masuma (Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh) , Hossain, Md. Sanowar (Department of Pharmacy, Pabna University of Science and Technology, Pabna- 6600, Bangladesh) , Parvez, G.M.Masud (Department of Pharmacy, Pabna University of Science and Technology, Pabna- 6600, Bangladesh) , Sarker, Ashish Kumar (Department of Pharmacy, Pabna University of Science and Technology, Pabna- 6600, Bangladesh) , Abdur Rahman, Md. Aziz (Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh) , Mamun, Al (Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh) , Islam, Md. Ekramul (Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh)

Abstract ▼ AI-Helper

Among the major constituents of Leea rubra (Family Vitaceae) leaves, phenolic and flavonoind compounds are most important for therapeutic purposes and the plant parts have been used in traditional medicine to treat several diseases for long. Thus, in order to scientifically confirm the traditional uses of the L. rubra leaves, the present study was designed to investigate the efficacy of the isolated flavones against AAPH induced oxidative damage to pUC19 DNA by gel electrophoresis and antineoplastic activity was evaluated on Ehrlich ascites carcinoma (EAC) bearing Swiss albino mice by evaluating percentage inhibition of cell growth, morphological changes of EAC cells and hematological parameters of the mice. The isolation was carried out by column chromatography and structure was revealed by 1H-NMR and 13C NMR. The result shows that, the isolated compound was identified as myricetin 4'-methoxy-3-O-α-L-rhamnopyranoside based on previously reported data. The isolated flavone effectively inhibited AAPH-induced oxidative damage to DNA; because it could inhibit the formation of circular and linear forms of the DNA. In anti-proliferative assay, 76% growth inhibition of EAC cells was observed as compare to the control mice (p<0.05) at a dose 100 mg/kg body weight. Thus the isolated flavone showed great importance as a possible therapeutic agent in preventing oxidative damage to DNA and the chronic diseases caused by such DNA damage, and can also become important in cancer chemotherapy.Highlights•Myricetin 4'-methoxy-3-O-α-L-rhamnopyranoside was isolated from plant Leea rubra.•The compound significantly inhibited AAPH-induced oxidative damage to pUC19 DNA.•Inhibition of EAC tumor growth in Swiss albino mice model was significant.•Hematological parameters in the cancer bearing mice were restored to normal values.

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참고문헌 (44)

1 Chatti I. Limem I. Boubaker J. Skandrani I. Kilani S. Bhouri W. Phytochemical, antibacterial, antiproliferative, and antioxidant potentials and DNA damage-protecting activity of Acacia salicina extracts J. Med. Food 12 3 2009 675 683 10.1089/jmf.2008.0120 19627220

원문보기 상세보기
2 Uddin Chy M. Adnan M. Chowdhury M. Pagano E. Kamal A. Oh K. Central and peripheral pain intervention by Ophiorrhiza rugosa leaves: potential underlying mechanisms and insight into the role of pain modulators J. Ethnopharmacol. 276 2021 114182 10.1016/j.jep.2021.114182 33964360

상세보기
3 Bari M. Khandokar L. Haque E. Romano B. Capasso R. Seidel V. Ethnomedicinal uses, phytochemistry, and biological activities of plants of the genus Gynura J. Ethnopharmacol. 271 2021 113834 10.1016/j.jep.2021.113834 33465439

상세보기
4 Čipák L. Rauko P. Miadoková E. Čipáková I. Novotný L. Effects of flavonoids on cisplatin-induced apoptosis of HL-60 and L1210 leukemia cells Leuk. Res. 27 1 2003 65 72 10.1016/s0145-2126(02)00063-2 12479854

상세보기
5 Lobo V. Patil A. Phatak A. Chandra N. Free radicals, antioxidants and functional foods: impact on human health Phcog. Rev. 4 8 2010 118 10.4103/0973-7847.70902 22228951

상세보기
6 Degtyareva N. Heyburn L. Sterling J. Resnick M. Gordenin D. Doetsch P. Oxidative stress-induced mutagenesis in single-strand DNA occurs primarily at cytosines and is DNA polymerase zeta-dependent only for adenines and guanines Nucleic Acids Res. 41 19 2013 8995 9005 10.1093/nar/gkt671 23925127

상세보기
7 Newman D. Cragg G. Natural products as sources of new drugs over the 30 Years from 1981 to 2010 J. Nat. Prod. 75 3 2012 311 335 10.1021/np200906s 22316239

상세보기
8 Fernández J. Silván B. Entrialgo-Cadierno R. Villar C. Capasso R. Uranga J. Antiproliferative and palliative activity of flavonoids in colorectal cancer Biomed. Pharmacother. 143 2021 112241 10.1016/j.biopha.2021.112241 34649363

상세보기
9 Sinan K. Akpulat U. Aldahish A. Celik Altunoglu Y. Baloğlu M. Zheleva-Dimitrova D. LC-MS/HRMS analysis, anti-cancer, anti-enzymatic and anti-oxidant effects of Boerhavia diffusa extracts: a potential raw material for functional applications 2003 Antioxidants 10 12 2021 10.3390/antiox10122003

상세보기
10 Rahman M. Reza A. Khan M. Sujon K. Sharmin R. Rashid M. Unfolding the apoptotic mechanism of antioxidant enriched-leaves of Tabebuia pallida (lindl.) miers in EAC cells and mouse model J. Ethnopharmacol. 278 2021 114297 10.1016/j.jep.2021.114297 34118341

상세보기
11 Tallei T. Fatimawali Niode N. Idroes R. Zidan B. Mitra S. A comprehensive review of the potential use of green tea polyphenols in the management of COVID-19 Evid. base Compl. Alternative Med. 2021 2021 1 13 10.1155/2021/7170736

상세보기
12 Ağagündüz D. Çelik M. Çıtar Dazıroğlu M. Capasso R. Emergent drug and nutrition interactions in COVID-19: a comprehensive narrative review Nutrients 13 5 2021 1550 10.3390/nu13051550 34064534

상세보기
13 Mousavi S. Karami A. Haghighi T. Tumilaar S. Fatimawali Idroes R. In silico evaluation of Iranian medicinal plant phytoconstituents as inhibitors against main protease and the receptor-Binding domain of SARS-CoV-2 Molecules 26 18 2021 5724 10.3390/molecules26185724 34577194

상세보기
14 Reddy N. Navanesan S. Sinniah S. Wahab N. Sim K. Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves BMC Compl. Alternative Med. 12 1 2012 10.1186/1472-6882-12-128

상세보기
15 Das N. Hossain S. Barmon J. Parvin S. Hasan M. Akter M. Islam E. Evaluation of leea rubra leaf extract for oxidative damage protection and antitumor and antimicrobial potential J. Trop. Med. 2021 2021 1 8 10.1155/2021/7239291

상세보기
16 Ongsakul M. Jindarat A. Rojanaworarit C. Antibacterial effect of crude alcoholic and aqueous extracts of six medicinal plants against Staphylococcus aureus and Escherichia coli J. Health Res. 23 3 2018 153 156 Retrieved from https://he01.tci-thaijo.org/index.php/jhealthres/article/view/156513
17 Seidel V. Bailleul F. Waterman P. Rel)-1β,2α-di-(2,4-dihydroxy-6-methoxybenzoyl)-3β, 4α-di-(4-methoxyphenyl)-cyclobutane and other flavonoids from the aerial parts of Goniothalamus gardneri and Goniothalamus thwaitesii Phytochemistry 55 5 2000 439 446 10.1016/s0031-9422(00)00346-0 11140605

상세보기
18 Xu R. Yang X. Wang J. Zhao H. Lu W. Cui J. Chemical composition and antioxidant activities of three polysaccharide fractions from pine cones Int. J. Mol. Sci. 13 12 2012 14262 14277 10.3390/ijms131114262 23203063

상세보기
19 Kumar S. Pandey A. Chemistry and biological activities of flavonoids: an overview 2013 Sci. World J. 2013 1 16 10.1155/2013/162750

상세보기
20 Zhao F. Liu Z. The protective effect of hydroxyl-substituted Schiff bases on the radical-induced oxidation of DNA J. Phys. Org. Chem. 22 9 2009 791 798 10.1002/poc.1517

상세보기
21 Islam F. Khatun H. Ghosh S. Ali M. Khanam J. Bioassay of Eucalyptus extracts for anticancer activity against Ehrlich ascites carcinoma (eac) cells in Swiss albino mice Asian.Pacific J. Trop. Biomed. 2 5 2012 394 398 10.1016/s2221-1691(12)60063-x

상세보기
22 Litchfield J.T. Jr. Wilcoxon F. A simplified method of evaluating dose-effect experiments J. Pharmacol. Exp. Therapeut. 96 2 1949 99 113
23 Heneczkowski M. Kopacz M. Nowak D. Kuźniar A. Infrared spectrum analysis of some flavonoids Acta Pol. Pharm. 58 6 2001 415 420 12197612

상세보기
24 El Antri A. Lachkar N. El Hajjaji H. Gaamoussi F. Lyoussi B. El Bali B. Structure elucidation and vasodilator activity of methoxy flavonols from Calycotome villosa subsp. intermedia Arab. J. Chem. 3 3 2010 173 178 10.1016/j.arabjc.2010.04.006

상세보기
25 Myricetin Retrieved, from https://pubchem.ncbi.nlm.nih.gov/compound/Myricetin 2022
26 Youssef Moustafa A. Khodair A. Saleh M. Isolation, structural elucidation of flavonoid constituents from Leptadenia pyrotechnica and evaluation of their toxicity and antitumor activity Pharmaceut. Biol. 47 6 2009 539 552 10.1080/13880200902875065

상세보기
27 Hossain F. Mostofa M. Alam A. Traditional uses and pharmacological activities of the genus leea and its phytochemicals: a review Heliyon 7 2 2021 e06222 10.1016/j.heliyon.2021.e06222

상세보기
28 Semwal D. Semwal R. Combrinck S. Viljoen A. Myricetin: a dietary molecule with diverse biological activities Nutrients 8 2 2016 90 10.3390/nu8020090 26891321

상세보기
29 Freitas M. Vasconcelos A. Gonçalves E. Ferrarini E. Vieira G. Cicia D. Involvement of opioid system and TRPM8/TRPA1 channels in the antinociceptive effect of spirulina platensis Biomolecules 11 4 2021 592 10.3390/biom11040592 33920609

상세보기
30 Praticò D. Delanty N. Oxidative injury in diseases of the central nervous system: focus on alzheimer’s disease Am. J. Med. 109 7 2000 577 585 10.1016/s0002-9343(00)00547-7 11063960

상세보기
31 Ahmed S. Khan H. Aschner M. Mirzae H. Küpeli Akkol E. Capasso R. Anticancer potential of furanocoumarins: mechanistic and therapeutic aspects Int. J. Mol. Sci. 21 16 2020 5622 10.3390/ijms21165622

상세보기
32 Yoshioka Y. Li X. Zhang T. Mitani T. Yasuda M. Nanba F. Black soybean seed coat polyphenols prevent AAPH-induced oxidative DNA-damage in HepG2 cells J. Clin. Biochem. Nutr. 60 2 2017 108 114 10.3164/jcbn.16-48 28366989

상세보기
33 Soumya K. Haridas K. James J. Sameer Kumar V. Edatt L. Sudheesh S. Study ofIn vitroantioxidant and DNA damage protection activity of a novel luteolin derivative isolated fromTerminalia chebula J. Taibah.Univ.Sci 13 1 2019 755 763 10.1080/16583655.2019.1630892

상세보기
34 Zhu L. Chen J. Tan J. Liu X. Wang B. Flavonoids from agrimonia pilosa ledeb: free radical scavenging and DNA oxidative damage protection activities and analysis of Bioactivity-structure relationship based on molecular and electronic structures Molecules 22 3 2017 195 10.3390/molecules22030195

상세보기
35 Wallet J.C. Cody V. Wojtczak A. Blessing R.H. Structural and conformational studies on bio-active flavonoids. Crystal and molecular structure of a complex formed between 2',6'-dimethoxyflavone and orthophosphoric acid: a model for flavone-nucleotide interactions Anti Cancer Drug Des. 8 5 1993 325 332
36 A.D. Sarma, R. Sharma, Anthocyanin-DNA copigmentation complex: mutual protection against oxidative damage (1999). Phytochemistry, 52(7) 1313-1318.
37 Küpeli Akkol E. Genç Y. Karpuz B. Sobarzo-Sánchez E. Capasso R. Coumarins and coumarin-related compounds in pharmacotherapy of cancer Cancers 12 7 2020 1959 10.3390/cancers12071959

상세보기
38 Tiwari P. Mishra Role of flavonoids in DNA damage and carcinogenesis prevention 08(04 J. Carcinog. Mutagen. 2017 10.4172/2157-2518.1000297

상세보기
39 Leong L. Shui G. An investigation of antioxidant capacity of fruits in Singapore markets Food Chem. 76 1 2002 69 75 10.1016/s0308-8146(01)00251-5

상세보기
40 Ozaslan M. Karagoz I.D. Kilic I.H. Guldur M.E. Ehrlich ascites carcinoma Afr. J. Biotechnol. 10 13 2011 2375 2378
41 Wong R. Apoptosis in cancer: from pathogenesis to treatment J. Exp. Clin. Cancer Res. 30 1 2011 10.1186/1756-9966-30-87

상세보기
42 Greenwell M. Rahman P.K. Medicinal plants: their use in anticancer treatment Int. J. Pharmaceut. Sci. Res. 6 10 2015 4103 4112 10.13040/IJPSR.0975-8232.6(10).4103-12
43 Goni O. Khan M. Rahman M. Hasan M. Kader F. Sazzad N. Pharmacological insights on the antidepressant, anxiolytic and aphrodisiac potentials of Aglaonema hookerianum Schott J. Ethnopharmacol. 268 2021 113664 10.1016/j.jep.2020.113664 33278545

상세보기
44 Küpeli Akkol E. Tatlı Çankaya I. Şeker Karatoprak G. Carpar E. Sobarzo-Sánchez E. Capasso R. Natural compounds as medical strategies in the prevention and treatment of psychiatric disorders seen in neurological diseases Front. Pharmacol. 12 2021 10.3389/fphar.2021.669638

상세보기

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A flavone from the ethyl acetate extract of Leea rubra leaves with DNA damage protection and antineoplastic activity 원문보기

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