[논문]Specific inhibition of Na+, K+-ATPase activity by atractylon, a major component of Byaku-jutsu, by interaction with enzyme in the E2 state

doi:10.1016/0006-2952(95)02188-4

Specific inhibition of Na+, K+-ATPase activity by atractylon, a major component of Byaku-jutsu, by interaction with enzyme in the E2 state

Biochemical pharmacology, v.51 no.3, 1996년, pp.339 - 343

Satoh, Kanako (Corresponding author: Dr. Kanako Satoh, Department of Toxicology, The Tokyo Metropolitan Research Laboratory of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169, Japan. Tel. 3-3363-3231, Ext. 425) , Nagai, Fumiko (FAX 3-3368-4060.) , Ushiyama, Keiko (Department of Toxicology, the Tokyo Metropolitan Research Laboratory of Public Health, Tokyo 169, Japan) , Kano, Itsu (Department of Toxicology, the Tokyo Metropolitan Research Laboratory of Public Health, Tokyo 169, Japan)

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AbstractAtractylon, a major component of the crude drug “Byaku-jutsu” (rhizomes of Atractylodes japonica), strongly inhibited Na+,K+-ATPase activity with an I50 value of 8.9 × 10−6 M. It also inhibited Mg2+ ATPase, H+,K+-ATPase, H+-ATPase and Ca2+-ATPase activities, but less potently. No effects on alkaline and acid phosphatase activities were observed. The inhibition of Na+,K+-ATPase activity by atractylon was non-competitive with respect to ATP and was greater with increasing K+ concentration, whereas it was not affected by Na+ concentration. The activity of K+-dependent p-nitrophenyl phosphatase, a partial reaction of Na+,K+-ATPase, was inhibited noncompetitively with respect to substrate (I50 value of 1.8 × 10−5 M), and the inhibition rate was independent of the K+ concentration. Furthermore, atractylon increased the Ki value for Na+ from 130 to 190 mM, but did not alter the Ki value for ATP. Inhibition of the phosphoenzyme formation by atractylon was greater at 0.1 M than at 1 M NaCl. K+-dependent dephosphorylation (E2-P to K · E2) was inhibited by atractylon, whereas ADP-sensitive (Na · E1-P to Na · E1) and non-specific dephosphorylation steps were not affected. These results suggest that atractylon, a specific inhibitor of Na+,K+-ATPase, interacts with enzyme in the E2 state and inhibits the reaction step from E2-P to K · E2.

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참고문헌 (26)

Nippon Toyo Igaku Zasshi Utizumi 4 1 1953 The study on diuretic crude drugs
Gifu Prefec Med School Bull Tsurumi 11 138 1963 On the diuretic action of crude drugs. II. Alcohol extracts
Yakugaku Zasshi Satoh 111 138 1991 10.1248/yakushi1947.111.2_138 The effects of crude drugs using diuretic on horse kidney (Na+ + K+)-adenosine triphosphatase

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Chem Pharm Bull (Tokyo) Hikino 10 641 1962 10.1248/cpb.10.641 Structure and autoxidation of atractylon

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Shoyakugaku Zasshi Nishikawa 30 132 1976 Studies on the evaluation of crude drugs. II. Identification of the ingredients of Atractylodes by thin-layer chromatography, gas chromatography and gas chromatography mass spectrometry, and the physical and chemical evaluation
Planta Med Pachaly 55 59 1989 10.1055/s-2006-961826 Inhaltsstoffe von Atractylis Koreana

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Biol Pharm Bull Sakurai 16 142 1993 10.1248/bpb.16.142 Enzyme inhibitory activities of acetylene and sesquiterpene compounds in Atractylodes Rhizome

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J Med Pharm Soc Wakan-Yaku Yasuda 7 420 1990 The inhibitory effects of Atractylodes Rhizoma on kidney Na+,K+-ATPase and its inhibitory constituents
Planta Med Kiso 2 97 1985 10.1055/s-2007-969416 Mechanism of antihepatotoxic activity of atractylon. I: Effect on free radical generation and lipid peroxidation

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Arch Pharm Res Sin 12 236 1989 10.1007/BF02911051 Pharmacological activities of the constituents of Atractylodes Rhizomes

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Yakugaku Zasshi Matsuda 111 36 1991 10.1248/yakushi1947.111.1_36 Imaging analysis of antiulcer action and the active constituent of Atractylodis Rhizoma

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J Biochem (Tokyo) Nakao 94 689 1983 10.1093/oxfordjournals.jbchem.a134408 Sodium and potassium ion-dependent change in oligomerization of Na,K-ATPase in C12E8 detected by low-angle laser light scattering technique in combination with high performance porous silica-gel chromatography

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J Biol Chem Maeda 263 3652 1988 10.1016/S0021-9258(18)68974-4 Modification of gastric (H+ + K+)-ATPase with pyridoxal 5′-phosphate

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Methods Enzymol Sweadner 156 65 1988 10.1016/0076-6879(88)56009-3 Preparation of the α(+) isozyme of the Na+,K+-ATPase from mammalian axolemma
Biochim Biophys Acta Satoh 994 104 1989 10.1016/0167-4838(89)90149-0 A monoclonal antibody against horse kidney (Na+ + K+)-ATPase inhibits sodium pump and E2K to E1 conversion of (Na+ + K+)-ATPase from outside of the cell membrane

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Biochem Pharmacol Satoh 44 373 1992 10.1016/0006-2952(92)90022-B Inhibition of Na+, K+-ATPase activity by β-eudesmol, a major component of Atractylodis Lanceae Rhizoma, due to the interaction with enzyme in the Na · E1 state

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Methods Enzymol Smith 10 81 1967 10.1016/0076-6879(67)10016-5 Preparation, properties, and conditions for assay of mitochondria: Slaughterhouse material, small scale
Biochem Pharmacol Nagai 36 3433 1987 10.1016/0006-2952(87)90322-4 Inhibition of calmodulin stimulation of phosphodiesterase and Ca2+,Mg2+-ATPase activities and shape change of erythrocyte ghosts by chloroquine

상세보기
Fernley Vol. 4 417 1971 Mammalian alkaline phosphatases
Hollander Vol. 4 449 1971 Acid phosphatases
Biochem Pharmacol Satoh 44 993 1992 10.1016/0006-2952(92)90134-5 Interaction of β-eudesmol with Na+,K+-ATPase: Inhibition of K+-pNPPase

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Biochim Biophys Acta Nagai 128 410 1966 10.1016/0926-6593(66)90193-7 Biphasic effects of nucleotides on potassium-dependent phosphatase

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J Gen Physiol Post 54 306s 1969 10.1085/jgp.54.1.306 Flexibility of an active center in sodium-plus-potassium adenosine triphosphatase

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J Biol Chem Taniguchi 250 3010 1975 10.1016/S0021-9258(19)41587-1 Synthesis of adenosine triphosphate and exchange between inorganic phosphate and adenosine triphosphate in sodium and potassium ion transport adenosine triphosphatase

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J Biochem (Tokyo) Hara 90 923 1981 10.1093/oxfordjournals.jbchem.a133580 Sodium ion discharge from pig kidney Na+,K+-ATPase. Na+-dependency of the E1P ？ E2P equilibrium in the absence of KCl

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Mol Pharmacol Yoda 22 693 1982 Formation of ADP-sensitive phosphorylated intermediate in the electric eel Na,K-ATPase preparation

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Specific inhibition of Na+, K+-ATPase activity by atractylon, a major component of Byaku-jutsu, by interaction with enzyme in the E2 state

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