[논문]Atractylenolide II induces G1 cell-cycle arrest and apoptosis in B16 melanoma cells

Ye, Y.; Wang, H.; Chu, J.H.; Chou, G.x.; Chen, S.B.; Mo, H.; Fong, W.f.; Yu, Z.L.

doi:10.1016/j.jep.2011.04.020

Atractylenolide II induces G1 cell-cycle arrest and apoptosis in B16 melanoma cells

Journal of ethnopharmacology, v.136 no.1, 2011년, pp.279 - 282

Ye, Y. (Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China) , Wang, H. , Chu, J.H. , Chou, G.x. , Chen, S.B. , Mo, H. , Fong, W.f. , Yu, Z.L.

Abstract ▼ AI-Helper

Ethnopharmacological relevance: Atractylenolide II (AT-II) is a sesquiterpene compound isolated from the dried rhizome of Atractylodes macrocephala (Baizhu in Chinese), which is traditionally prescribed for melanoma treatment by Chinese medicine practitioners. Our previous study showed that AT-II can inhibit B16 cells proliferation. Here we investigate the mechanistic basis for the anti-proliferative activity of AT-II in B16 melanoma cells. Materials and methods: Cell viability was examined by MTT assay. Cell cycle distribution and apoptosis were determined by flow cytometry. Protein expression was determined by Western blotting. Results: AT-II treatment for 48h dose-dependently inhibited cell proliferation with an IC50 of 82.3μM, and induced G1 phase cell cycle arrest. Moreover, treatment with 75μM AT-II induced apoptosis. These observations were associated with the decrease of the expression of Cdk2, phosphorylated-Akt, phosphorylated-ERK and Bcl-2, the increase of the expression of phosphorylated-p38, phosphorylated-p53, p21, p27, and activation of caspases-8, -9 and -3. In addition, a chemical inhibitor of p53, PFTα, significantly decreased AT-II-mediated growth inhibition and apoptosis. Conclusions: We demonstrated that the G1-arresting and apoptotic effects of AT-II in B16 cells involve p38 activation as well as ERK and Akt inactivation, and the cytotoxic/apoptotic effects of AT-II are potentially p53 dependent. These findings provided chemical and pharmacological basis for the traditional application of Baizhu in melanoma treatment.

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참고문헌 (25)

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Atractylenolide II induces G1 cell-cycle arrest and apoptosis in B16 melanoma cells

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