[논문]Elective and selective media for lactic acid bacteria

Reuter, G.

doi:10.1016/0168-1605(85)90057-1

Elective and selective media for lactic acid bacteria

International journal of food microbiology, v.2 no.1/2, 1985년, pp.55 - 68

Reuter, G.

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Lactic acid bacteria comprise a heterogeneous group of microorganisms. Isolates from food include representatives of the genera Lactobacillus, Streptococcus, Pediococcus and Leuconostoc. As no universal selective medium exists for the cultivation of all lactic acid bacteria the well-known media for lactobacilli may be modified by changing the pH value, the concentrations of inhibitory agents and the incubation time and temperature. It may be necessary to select media for special fields of application, e.g. milk and milk products, meat and meat products. Surface plating under microaerophilic or anaerobic conditions is generally recommended. Generally suitable as selective media for lactic acid bacteria are MRS-medium, adapted to a lower pH (e.g. 5.7) with the addition of an inhibitor (e.g. sorbic acid) or a modified Rogosamedium with increased pH (e.g. 6.2). The LaS (lactobacilli-sorbic acid) medium (pH 5.0) incubated for at least 48 h is also suitable but fails to recover enterococci. However, this can be compensated by a special enterococcus medium used in parallel. Elective media for defined groups of lactic acid bacteria such as lactic streptococci or yoghurt bacteria have recently been used successfully. One of those is the M 17 medium for lactic streptococci which must be used strictly according to the recommendations of the authors. Good growth can also be obtained with the BRIGGS or RCM medium, the latter supplemented with 1% dextrose or lactose. For the differentiation of the yoghurt lactic acid bacteria the TPPY medium proved suitable as well as the LSD medium which was introduced recently. No special medium can be recommended at present for the selective cultivation of leuconostocs or pediococci. Selection of media has to be determined by the microflora of the habitat to be investigated considering the individual experience of the investigator. It seems reasonable in many cases to use two media at the same time. Quality assurance of media must be carried out regularly using test strains to check selectivity and productivity. The easiest way is a semi-quantitative loop streaking technique which may be used in different modifications. The confirmation of testing can be done by a quantitative drop-plating procedure.

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참고문헌 (27)

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Elective and selective media for lactic acid bacteria

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