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Abstract ▼ AI-Helper

To develop an efficient protocol for the transformation of the Taraxacum mongolicum H. Mazz, hypocotyls taken from 6-day old plantlets were infected with Agrobacterium tumefaciens LBA4404 harboring the binary vector which carries the reporter gene EGFP (enhanced green fluorescent protein) and the ka...

To develop an efficient protocol for the transformation of the Taraxacum mongolicum H. Mazz, hypocotyls taken from 6-day old plantlets were infected with Agrobacterium tumefaciens LBA4404 harboring the binary vector which carries the reporter gene EGFP (enhanced green fluorescent protein) and the kanamycin resistance gene npt Ⅱ. Infected explants were cocultured on shoot induction medium with 0.5 ㎎ㆍL⁻½ NAA, 1.0 ㎎ㆍL⁻½ BA (SI medium). After 2 days of culture, explants were transferred to SM medium (SI medium with 50 ㎎ㆍL⁻½ kanamycin). Putative transformed shoots were selected on SM medium. Integration of EGFP gene into host plant chromosome was confirmed through genomic PCR with specific primer in EGFP gene and npt II gene. In addition, expression of introduced EGFP gene was observed in transformed Taraxacum mongolicum using fluorescent microscope. These results suggest that transformants containing the useful foreign gene could be produced using Agrobacterium tumefaciens-mediated transformation system in Taraxacum mongolicum.

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