[논문]The effect of 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) on the development and chromosomal complement of sheep parthenogenetic and nuclear transfer embryos

Alexander, B.; Coppola, G.; Di Berardino, D.; Rho, G.J.; St John, E.; Betts, D.H.; King, W.A.

doi:10.1002/mrd.20372

[해외논문] The effect of 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) on the development and chromosomal complement of sheep parthenogenetic and nuclear transfer embryos

Molecular reproduction and development, v.73 no.1, 2006년, pp.20 - 30

Alexander, B. (Department of Biomedical Science, Ontario Veterinary College, University of Guelph, Ontario, Canada) , Coppola, G. (Department of Biomedical Science, Ontario Veterinary College, University of Guelph, Ontario, Canada) , Di Berardino, D. (Department of Animal Sciences and Food Inspection, Laboratory of Animal Cytogenetics, University of Naples, Federico II, Portici, Italy) , Rho, G.J. (Department of Biology, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea) , St John, E. (Department of Biomedical Science, Ontario Veterinary College, University of Guelph, Ontario, Canada) , Betts, D.H. (Department of Biomedical Science, Ontario Veterinary College, University of Guelph, Ontario, Canada) , King, W.A. (Department of Biomedical Science, Ontario Veterinary College, University of Guelph, Ontario, Canada)

Abstract ▼ AI-Helper

The effects of activation by 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) on the development and chromosomal complement of sheep parthenogenetic and SCNT embryos were investigated. The results revealed that the blastocyst development of parthenogenetic embryos was significantly higher (P 0.05) between the two activation treatment groups for SCNT embryos. The 6-DMAP or CHX treatment did not result in any significant difference in the blastocyst total cell number in either parthenote or SCNT embryos. The chromosomal analysis revealed that all the parthenogenetic embryos (100.0%) derived from 6-DMAP treatment, were chromosomally abnormal whereas in CHX-treated embryos, it was significantly lowered (93.6%, P 0.05) among the 6-DMAP and CHX- treated embryo groups (60.0% vs. 56.2%, respectively). This study demonstrated that oocyte activation agents such as DMAP and CHX have differing effects on meiotic or mitotic nuclei. The study also highlighted the feasibility of using bovine X and Y chromosome specific painting probes in sheep embryos. Mol. Reprod. Dev. © 2005 Wiley-Liss, Inc.

주제어

참고문헌 (43)

Biol Reprod Arat 66 1768 2002 10.1095/biolreprod66.6.1768

상세보기
Mol Reprod Dev Barnes 36 33 1993 10.1002/mrd.1080360106

상세보기
Cloning Booth 2 63 2000 10.1089/152045500436087

상세보기
Biol Reprod Booth 68 922 2003 10.1095/biolreprod.102.007187

상세보기
Reprod Fertil Dev Cha 9 441 1997 10.1071/R96078

상세보기
Oxford Rev Reprod Biol Chandley 6 1 1984
J Cell Sci Compton 111 1477 1998 10.1242/jcs.111.11.1477

상세보기
Reproduction in mammals and man Crozet 328 1993 1993. Fertilization in vivo and in vitro. In: , , editors. Reproduction in mammals and man. Paris: Ellipses. pp 328-347.
Biol Reprod De La Fuente 58 952 1998 10.1095/biolreprod58.4.952

상세보기
Hum Reprod Delhanty 3 201 1995 10.1093/humupd/1.3.201
Mol Reprod Dev Di Berardino 67 108 2004 10.1002/mrd.20016

상세보기
Biol Reprod Gibbons 66 895 2002 10.1095/biolreprod66.4.895

상세보기
Biol Reprod Gordo 62 1370 2000 10.1095/biolreprod62.5.1370

상세보기
Mol Reprod Dev Grupen 62 387 2002 10.1002/mrd.10126

상세보기
Cloning Hill 1 201 2000 10.1089/15204559950019834

상세보기
Cloning and Stem Cells Holm 5 133 2003 10.1089/153623003322234731

상세보기
J Cell Sci Hyman 111 2077 1998 10.1242/jcs.111.15.2077

상세보기
Cell Jacobson 88 347 1997 10.1016/S0092-8674(00)81873-5

상세보기
Advances in Veterinary Science and Comparative Medicine King 34 229 1990 1990. Chromosome abnormalities and pregnancy failure in domestic animals. In: editor. Advances in Veterinary Science and Comparative Medicine. San Diego, CA: Academic Press. 34: 229-250.
Biol Reprod Lagutina 70 400 2004 10.1095/biolreprod.103.021972

상세보기
Biol Reprod Loi 58 1177 1998 10.1095/biolreprod58.5.1177

상세보기
Mol Reprod Dev Long 36 23 1993 10.1002/mrd.1080360105

상세보기
J Exp Zool McGaughey 176 383 1971 10.1002/jez.1401760402

상세보기
Biol Reprod Mitalipov 60 821 1999 10.1095/biolreprod60.4.821

상세보기
J Vet Med Sci Mohamed 62 339 2000 10.1292/jvms.62.339

상세보기
Morris 1998 1998. Effect of male in an in vitro embryo production system. DVSc Thesis, University of Guelph, Guelph, Ontario, Canada.
Theriogenology Motlik 49 461 1998 10.1016/S0093-691X(97)00418-4

상세보기
Nature Murray 359 599 1992 10.1038/359599a0

상세보기
J Reprod Fert Murray 78 439 1986 10.1530/jrf.0.0780439

상세보기
Dev Biol Navara 162 29 1994 10.1006/dbio.1994.1064

상세보기
Theriogenology O'Brian 47 1433 1997 10.1016/S0093-691X(97)00134-9

상세보기
Reprod Fertil Dev Peura 15 157 2003 10.1071/RD02092

상세보기
Proc Natl Acad Sci USA Pinto-Correia 91 7894 1994 10.1073/pnas.91.17.7894

상세보기
Mol Reprod Dev Presicce 37 61 1994 10.1002/mrd.1080370109

상세보기
Biol Reprod Ptak 61 1568 1999 10.1095/biolreprod61.6.1568

상세보기
Proc Natl Acad Sci USA Sathananthan 88 4806 1991 10.1073/pnas.88.11.4806

상세보기
Science Simerly 300 297 2003 10.1126/science.1082091

상세보기
Clon and Stem Cells Slimane- Bureau 5 123 2003 10.1089/153623003322234722

상세보기
Biol Reprod Stice 39 657 1988 10.1095/biolreprod39.3.657

상세보기
Dev Bio Susko-Parrish 166 729 1994 10.1006/dbio.1994.1351

상세보기
Biol Reprod Walker 55 703 1996 10.1095/biolreprod55.3.703

상세보기
Nature Wilmut 385 810 1997 10.1038/385810a0

상세보기
Develop Genetics Winger 21 160 1997 10.1002/(SICI)1520-6408(1997)21:2<160::AID-DVG5>3.0.CO;2-5

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[해외논문] The effect of 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) on the development and chromosomal complement of sheep parthenogenetic and nuclear transfer embryos

Abstract ▼ AI-Helper

주제어

참고문헌 (43)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트