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Abstract ▼ AI-Helper

Phenylethyl isothiocyanate, a isothiocyanate found in chinese cabbage (Brassica campestris), was reported to have a potent cytotoxicity and to be able to induce apoptosis against HL-60 human leukemic cell line. Sulforaphane (1-isothiocyanato-4-(methylsulfinyl)-butane), is a metabolite of the glucosi...

Phenylethyl isothiocyanate, a isothiocyanate found in chinese cabbage (Brassica campestris), was reported to have a potent cytotoxicity and to be able to induce apoptosis against HL-60 human leukemic cell line. Sulforaphane (1-isothiocyanato-4-(methylsulfinyl)-butane), is a metabolite of the glucosinolate-glucoraphainin by myrosinase in chinese cabbage. Hence, it would be possible to produce functional chinese cabbages having high sulforaphane contents by means of inducing myrosinase over-expression in the plant. In this study, we developed quantitative real-time PCR detection method for the quantification of myrosinase mRNA level in the transformants. The detection primer set of the method showed high specificity to the target gene and produced 183 base-pairs of PCR amplicon. 26 transformants, Nicotiana tabacum having myrosinase sense or antisense genes, were analysed and quantified successfully. In conclusion, this method would not only facilitate to select the useful transformants but also assay effectively the quantification of expressed myrosinase in the transformants.

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