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The genetic relationship among 31 Sedum sarmentosum local strains was analyzed using randomly amplified polymorphic DNA (RAPD) with fifteen 10-mers random primers (Operon Technologies Inc., USA) and twelve 20-mers random primers (SeouLin Bioscience Inc., Korea). Forty-six polymorphic bands (3.3 per ...

The genetic relationship among 31 Sedum sarmentosum local strains was analyzed using randomly amplified polymorphic DNA (RAPD) with fifteen 10-mers random primers (Operon Technologies Inc., USA) and twelve 20-mers random primers (SeouLin Bioscience Inc., Korea). Forty-six polymorphic bands (3.3 per primer) and ninety (15.0 per primer) were generated by polymerase chain reaction with selected fourteen 10-mers primers, and six 20-mers primers, respectively. The production of polymorphic bands in 20-mers primers was more efficient than in 10-mers primers. The cluster analysis based on RAPD markers generated by 20-mers primers was matched well with morphological characteristics compared to 10-mers primer. Thirty-one local strains analyzed with UPGMA were clustered into 2 groups. Twenty-seven local strains were clustered into the group Ⅰ, and the geneticrelationship in Group I was very closed except Wanju and Gunsan local strains as no-flowering. Group Ⅱ included Keumsan, Bucheon, Pocheon and Yongin local strains with long hight, slender stem, narrow leaf, and abundant flowering characteristics.

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