Park, Ji Kwon
(Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National university, JinJu, Korea.)
,
Shin, Jeong Kyu
(Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National university, JinJu, Korea.)
,
Choi, Won Jun
(Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National university, JinJu, Korea.)
,
Lee, Soon Ae
(Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National university, JinJu, Korea.)
,
Lee, Jong Hak
(Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National university, JinJu, Korea.)
,
Paik, Won Young
(Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National university, JinJu, Korea.)
OBJECTIVE: To evaluate the expression level, localization and distribution of 14-3-3sigma and zeta proteins in human ovarian epithelial carcinoma cells. METHODS: Epithelial carcinoma, benign epithelial tumor and normal ovarian tissues were evaluated by immunohistochemistry. For this study, mucinous ...
OBJECTIVE: To evaluate the expression level, localization and distribution of 14-3-3sigma and zeta proteins in human ovarian epithelial carcinoma cells. METHODS: Epithelial carcinoma, benign epithelial tumor and normal ovarian tissues were evaluated by immunohistochemistry. For this study, mucinous adenoma (n=10), serous adenoma (n=10), mucinous adenocarcinoma (n=10) and serous adenocarcinoma (n=10) of ovary and normal ovarian tissue (n=10) were analyzed. Goat anti 14-3-3sigma polyclonal antibody and rabbit anti-14-3-3zeta polyclonal antibody were used as primary antibodies. RESULTS: The 14-3-3sigma protein expression in the glandular epithelium was not statistically different among normal ovary, cystadenoma and cystadenocarcinoma. The 14-3-3zeta protein expression in the glandular epithelium was significantly higher for cystadenocarcinoma than for cystadenoma and normal ovary. The 14-3-3sigma and zeta proteins were immunohistochemically demonstrated in the nuclei and more preferably in the cytoplasm of adenocarcinoma epithelial cells. CONCLUSION: The expression of 14-3-3zeta protein increased significantly in ovarian adenocarcinoma compared with adenoma and normal ovary. It is suggested that 14-3-3zeta protein expression may play an important role in abnormal growth of ovarian epithelial tumor cell.
OBJECTIVE: To evaluate the expression level, localization and distribution of 14-3-3sigma and zeta proteins in human ovarian epithelial carcinoma cells. METHODS: Epithelial carcinoma, benign epithelial tumor and normal ovarian tissues were evaluated by immunohistochemistry. For this study, mucinous adenoma (n=10), serous adenoma (n=10), mucinous adenocarcinoma (n=10) and serous adenocarcinoma (n=10) of ovary and normal ovarian tissue (n=10) were analyzed. Goat anti 14-3-3sigma polyclonal antibody and rabbit anti-14-3-3zeta polyclonal antibody were used as primary antibodies. RESULTS: The 14-3-3sigma protein expression in the glandular epithelium was not statistically different among normal ovary, cystadenoma and cystadenocarcinoma. The 14-3-3zeta protein expression in the glandular epithelium was significantly higher for cystadenocarcinoma than for cystadenoma and normal ovary. The 14-3-3sigma and zeta proteins were immunohistochemically demonstrated in the nuclei and more preferably in the cytoplasm of adenocarcinoma epithelial cells. CONCLUSION: The expression of 14-3-3zeta protein increased significantly in ovarian adenocarcinoma compared with adenoma and normal ovary. It is suggested that 14-3-3zeta protein expression may play an important role in abnormal growth of ovarian epithelial tumor cell.
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