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To a better understanding for molecular mechanism of oncogenesis in hepatoma primary hepatocellular carcinoma and hepatoma cell lines(Hep 3B PLC/PRF /5 Hep G2) were subjected to detailed cytogenetic analysis with G-banding method after cell cultures No cloned chromosomal abnormalities were found in ...

To a better understanding for molecular mechanism of oncogenesis in hepatoma primary hepatocellular carcinoma and hepatoma cell lines(Hep 3B PLC/PRF /5 Hep G2) were subjected to detailed cytogenetic analysis with G-banding method after cell cultures No cloned chromosomal abnormalities were found in the primary hepatoma(below 10%) On the other hand all hepatoma cell lines were cloned the specific chromosomal abnormalities in Hep 3B were del(lp2l) del(6q14) and t(1 11)(p11 q13) Genes of AMY1A CGA SEA and HSTF1 were located on 1p21 and 6q14 respectively SEA and HSTF1 were located on 11q13 Regions of chromosome abnormalities in PLC/PRF /5 were the same found in Hep 3B Besides del(1q32) and del(1p32) were also cloned Gene of CRl and MYCL1 were located on 1q32 and 1p32 respectively The characteristic findings of chromosome abnormalities in Hep G2 were del(1p31) and del(1q22) And GST1 and DAF were located on these regions each other Del(6q11) and del(1p22) were also found in Hep G2 From the above results it is presumed that HBV may integrate to AMY1A gene or near this gene and leads to loss of functions to this gene And impaired regulation of CGA occurs in next step SEA HSTF1 and MYCL1 oncogenes may act as a progressing factor of tumourgenesis in HBsAg( + ) hepatoma Some factors like chemical agents may cause functional loss of GST1 and DAF at first and functional loss of cell regulation of CGA occurs in next step SKI oncogene may promote the progression of carcinogenesis to this cell line Whether any causative agents are involved in carcinogenesis of hepatoma functional loss of CGA gene is the most important factor in tumour-genesis in hepatoma.