잎에 모자이크 증상을 나타내는 개갓냉이(Roippa indica)와 겨자무(Armoracia lapathifolia)로부터 순무 모자이크 바이러스(TuMV)를 분리하였다. 분리한 바이러스를 지표식물 접종시험, 전자현미경 관찰, 항혈청 검정 및 RT-PCR을 통하여 TuMV로 동정하였다. 이들 바이러스 는 Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea 및 Raphanus sativus에 전신감염되었고, C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc, 및 Gomphrena grobosa의 접종엽에 국부병반을 나타냈으나, N. glutinosa, Cucumis sativus 및 Vigna unguiculata에는 병징이 나타나지 않았다. 이병엽의 조직에서 720 ㎚ 정도의 사상형 입자와 봉입체가 관찰되었으며, 한천겔이중확산법에 의한 TuMV와의 혈청반응에서 모두 양성반응을 나타냈다. 또한, 제작한 TuMV primer를 이용한 RT-PCR 검정에서도 1,092 bp의 PCR 산물이 확인되었다. 한편, 배추에서 분리한 TuMV를 7종의 십자화과 잡초에 접종한 결과, 냉이(Capsella bursa-pastoris), 말냉이 (Thlaspi arvense), 꽃다지 (Draba nomorosa var. hebecarpa), 좀개갓냉이(Rorippa cantoniensis), 나도냉이(Barbarea orthoceras), 장대나물(Arabis glabra) 등 6종의 잡초에 감염되었다.
잎에 모자이크 증상을 나타내는 개갓냉이(Roippa indica)와 겨자무(Armoracia lapathifolia)로부터 순무 모자이크 바이러스(TuMV)를 분리하였다. 분리한 바이러스를 지표식물 접종시험, 전자현미경 관찰, 항혈청 검정 및 RT-PCR을 통하여 TuMV로 동정하였다. 이들 바이러스 는 Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea 및 Raphanus sativus에 전신감염되었고, C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc, 및 Gomphrena grobosa의 접종엽에 국부병반을 나타냈으나, N. glutinosa, Cucumis sativus 및 Vigna unguiculata에는 병징이 나타나지 않았다. 이병엽의 조직에서 720 ㎚ 정도의 사상형 입자와 봉입체가 관찰되었으며, 한천겔이중확산법에 의한 TuMV와의 혈청반응에서 모두 양성반응을 나타냈다. 또한, 제작한 TuMV primer를 이용한 RT-PCR 검정에서도 1,092 bp의 PCR 산물이 확인되었다. 한편, 배추에서 분리한 TuMV를 7종의 십자화과 잡초에 접종한 결과, 냉이(Capsella bursa-pastoris), 말냉이 (Thlaspi arvense), 꽃다지 (Draba nomorosa var. hebecarpa), 좀개갓냉이(Rorippa cantoniensis), 나도냉이(Barbarea orthoceras), 장대나물(Arabis glabra) 등 6종의 잡초에 감염되었다.
Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-polymerase chain r...
Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-polymerase chain reaction (RT-PCR). Both viruses systemically infected Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea and Raphanus sativus, and developed local infection on inoculated leaves of C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc and Gomphrena grobosa. However, the viruses did not infect on N. glutinosa, Cucumis sativus and Vigna unguiculata. The filamentous particles, about 720 ㎚ in length, and inclusion bodies were observed from the infected leaf tissues by dipping on electron microscopy. Crude sap of leaf infected with the viruses was reacted positively with an antiserum of TuMV in agar gel double diffusion. For detection of the viruses, RT-PCR was carried out with TuMV-specific oligonucleotide primer. The RT-PCR products, a 1,092 bp DNA fragment, were obtained from naturally infected leaves of R. indica and A. lapathifolia. In inoculation test to seven cruciferous weeds with TuMV, infection occurred in Arabis glabra, Barbarea orthoceras, Capsella bursa-pastoris, Draba nomorosa var, hebecarpa, Rorippa cantoniensis and Thlaspi arvense.
Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-polymerase chain reaction (RT-PCR). Both viruses systemically infected Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea and Raphanus sativus, and developed local infection on inoculated leaves of C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc and Gomphrena grobosa. However, the viruses did not infect on N. glutinosa, Cucumis sativus and Vigna unguiculata. The filamentous particles, about 720 ㎚ in length, and inclusion bodies were observed from the infected leaf tissues by dipping on electron microscopy. Crude sap of leaf infected with the viruses was reacted positively with an antiserum of TuMV in agar gel double diffusion. For detection of the viruses, RT-PCR was carried out with TuMV-specific oligonucleotide primer. The RT-PCR products, a 1,092 bp DNA fragment, were obtained from naturally infected leaves of R. indica and A. lapathifolia. In inoculation test to seven cruciferous weeds with TuMV, infection occurred in Arabis glabra, Barbarea orthoceras, Capsella bursa-pastoris, Draba nomorosa var, hebecarpa, Rorippa cantoniensis and Thlaspi arvense.
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