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CB 8620, a strain of Erwinia carotovora subsp. carotovora, spontaneously produced a bacteriocin in some cultural media and its bacteriocinogenicity was accelerated by treatment of mitomycin-C. Syntheses of the bacteriocin which was designated as carotovoricin H were accompanied with cell lysis. Biol...

CB 8620, a strain of Erwinia carotovora subsp. carotovora, spontaneously produced a bacteriocin in some cultural media and its bacteriocinogenicity was accelerated by treatment of mitomycin-C. Syntheses of the bacteriocin which was designated as carotovoricin H were accompanied with cell lysis. Biological and chemical properties of carotovoricin H indicated that the bacteriocin is a thermolable and sensitive to sodium dodecyl sulfate. Carotovoricin H, however, was resistant to hydrolytic action by trypsin. Ultraviolet absorption spectrum of the purified bacteriocin was typical of a protein with absorption maximal at 280 ㎚. SDS-polyacrylamide gel electrophoresis of carotovoricin H revealed four major polypeptides. Electron micrographs of the purified carotovoricin H showed that its structure is a rod-shaped particle which consists of a sheath, a core, and fibers. The sheath appeared capable of contraction. (Accepted for publication December 3, 1987)

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