이 연구에서는 제1보의 실내냄새유발곰팡이의 원인 종 규명에 이어 확인된 Geotrichum sp.에서 방출되는 MVOCs (Microbial Volitile Organic Compounds)중 주요냄새원인물질을 확인하였다. Geotrichum sp.의 냄새물질을 채취하는 방법으로는 head space-solid phase microextraction (HS-SPME)방법을 사용하였다. 냄새물질의 확인은 GC/FID/Olfactometry방법으로 확인하였고, CG/AED방법으로 확인된 냄새물질 중 냄새물질들이 주로 포함하고 있는 산소, 질소, 황이 있는지 알아보았다. 마지막으로 GC/MS를 이용하여 냄새물질을 정성하고 각각의 냄새물질 RI (RetentionIndex)를 확인하였다. 그 결과 Geotrichum sp.의 MVOCs로는 hydrogen sulfide, methanthiol, 1-hutanol, carbon disulfide, dimethyl sulfide, dimethyl disulfide, S-methyl thiobutyrate, dimethyl trisulfide, geosmin이었으며, 확인된 주요냄새물질은 변 냄새를 유발하는 물질은 dimethyl disulfide와 dimethyl trisulfide였으며, 곰팡이 냄새를 유발하는 물질은 geosmin으로 확인되었다.
이 연구에서는 제1보의 실내냄새유발곰팡이의 원인 종 규명에 이어 확인된 Geotrichum sp.에서 방출되는 MVOCs (Microbial Volitile Organic Compounds)중 주요냄새원인물질을 확인하였다. Geotrichum sp.의 냄새물질을 채취하는 방법으로는 head space-solid phase microextraction (HS-SPME)방법을 사용하였다. 냄새물질의 확인은 GC/FID/Olfactometry방법으로 확인하였고, CG/AED방법으로 확인된 냄새물질 중 냄새물질들이 주로 포함하고 있는 산소, 질소, 황이 있는지 알아보았다. 마지막으로 GC/MS를 이용하여 냄새물질을 정성하고 각각의 냄새물질 RI (Retention Index)를 확인하였다. 그 결과 Geotrichum sp.의 MVOCs로는 hydrogen sulfide, methanthiol, 1-hutanol, carbon disulfide, dimethyl sulfide, dimethyl disulfide, S-methyl thiobutyrate, dimethyl trisulfide, geosmin이었으며, 확인된 주요냄새물질은 변 냄새를 유발하는 물질은 dimethyl disulfide와 dimethyl trisulfide였으며, 곰팡이 냄새를 유발하는 물질은 geosmin으로 확인되었다.
In this study, we identified the odor active compounds among MVOCs (Microbial Volitile Organic Compounds) emitted from Geotrichum sp. which was previously identified as the main kinds of mold in indoor air. To sample the odor active compounds emitted from Geotrichum sp. while metabolizing, head spac...
In this study, we identified the odor active compounds among MVOCs (Microbial Volitile Organic Compounds) emitted from Geotrichum sp. which was previously identified as the main kinds of mold in indoor air. To sample the odor active compounds emitted from Geotrichum sp. while metabolizing, head space-solid phase microextraction (HS-SPME) method, which is well known for sampling odorants, was used. GC/Flame Ionization Detector/Olfactometry was used to identify main odor active compounds. GC/Atomic Emission Detector was used to identify if some compounds had atoms like oxygen, nitrogen, sulfur which are typically found from odorants. GC/Mass Spectrometry was used to identify the odorants. Finally, Retention Index (RI) of each system was verified. In this study, hydrogen sulfide, methanthiol, 1-butanol, carbon disulfide, dimethyl sulfide, dimethyl disulfide, S-methyl thiobutyrate, dimethyl trisulfide, geosmin were identified as MVOCs. Among them, identified main odor active compounds were dimethyl disulfie and dimethyl trisulfide which induce ordure smell and geosmin which induce moldy, earthy smell.
In this study, we identified the odor active compounds among MVOCs (Microbial Volitile Organic Compounds) emitted from Geotrichum sp. which was previously identified as the main kinds of mold in indoor air. To sample the odor active compounds emitted from Geotrichum sp. while metabolizing, head space-solid phase microextraction (HS-SPME) method, which is well known for sampling odorants, was used. GC/Flame Ionization Detector/Olfactometry was used to identify main odor active compounds. GC/Atomic Emission Detector was used to identify if some compounds had atoms like oxygen, nitrogen, sulfur which are typically found from odorants. GC/Mass Spectrometry was used to identify the odorants. Finally, Retention Index (RI) of each system was verified. In this study, hydrogen sulfide, methanthiol, 1-butanol, carbon disulfide, dimethyl sulfide, dimethyl disulfide, S-methyl thiobutyrate, dimethyl trisulfide, geosmin were identified as MVOCs. Among them, identified main odor active compounds were dimethyl disulfie and dimethyl trisulfide which induce ordure smell and geosmin which induce moldy, earthy smell.
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