[논문]Control of postharvest soft rot caused by Erwinia carotovora of vegetables by a strain of Bacillus amyloliquefaciens and its potential modes of action

Zhao, Yancun; Li, Pengxia; Huang, Kaihong; Wang, Yuning; Hu, Huali; Sun, Ya

doi:10.1007/s11274-012-1193-0

Control of postharvest soft rot caused by Erwinia carotovora of vegetables by a strain of Bacillus amyloliquefaciens and its potential modes of action

World journal of microbiology & biotechnology, v.29 no.3, 2013년, pp.411 - 420

Zhao, Yancun , Li, Pengxia , Huang, Kaihong , Wang, Yuning , Hu, Huali , Sun, Ya

Abstract ▼ AI-Helper

Erwinia carotovora subsp. carotovora (Ecc), the causal agent of bacterial soft rot, is one of the destructive pathogens of postharvest vegetables. In this study, a bacterial isolate (BGP20) from the vegetable farm soil showed strong antagonistic activity against Ecc in vitro, and its twofold cell-free culture filtrate showed excellent biocontrol effect in controlling the postharvest bacterial soft rot of potatoes at 25 C. The anti-Ecc metabolites produced by the isolate BGP20 had a high resistance to high temperature, UV-light and protease K. Based on the colonial morphology, cellular morphology, sporulation, and partial nucleotide sequences of 16S rRNA and gyrB gene, the isolate BGP20 was identified as Bacillus amyloliquefaciens subsp. plantarum. Further in vivo assays showed that the BGP20 cell culture was more effective in controlling the postharvest bacterial soft rot of green peppers and Chinese cabbages than its twofold cell-free culture filtrate. In contrast, the biocontrol effect and safety of the BGP20 cell culture were very poor on potatoes. In the wounds of potatoes treated with both the antagonist BGP20 and the pathogen Ecc, the viable count of Ecc was 31,746 times that of BGP20 at 48 h of incubation at 25 C. But in the wounds of green peppers, the viable count of BGP20 increased 182.3 times within 48 h, and that of Ecc increased only 51.3 %. In addition, the treatment with both BGP20 and Ecc induced higher activity of phenylalanine ammonia-lyase (PAL) than others in potatoes. But the same treatment did not induce an increase of PAL activity in green peppers. In conclusion, the present study demonstrated that the isolate BGP20 is a promising candidate in biological control of postharvest bacterial soft rot of vegetables, but its main mode of action is different among various vegetables.

참고문헌 (38)

Biol Control AO Abraham 53 32 2010 10.1016/j.biocontrol.2009.12.009 Abraham AO, Laing MD, Bower JP (2010) Isolation and in vivo screening of yeast and Bacillus antagonists for the control of Penicillium digitatum of citrus fruit. Biol Control 53:32-38

상세보기
Crop Prot E Arrebola 29 369 2010 10.1016/j.cropro.2009.08.001 Arrebola E, Sivakumar D, Bacigalupo R, Korsten L (2010) Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases. Crop Prot 29:369-377

상세보기
Postharvest Biol Tec JS Assis 23 33 2001 10.1016/S0925-5214(01)00100-4 Assis JS, Maldonado R, Muñoz T, Escribano MI, Merodio C (2001) Effect of high carbon dioxide concentration on PAL activity and phenolic contents in ripening cherimoya fruit. Postharvest Biol Tec 23:33-39

상세보기
Asian J Plant Sci KA Bhat 9 4 200 2010 10.3923/ajps.2010.200.208 Bhat KA, Masood SD, Bhat NA, Bhat MA, Razvi SM, Mir MR, Akhtar S, Wani N, Habib M (2010) Current status of post harvest soft rot in vegetables: a review. Asian J Plant Sci 9(4):200-208

상세보기
Postharvest Biol Tec C Casals 64 126 2012 10.1016/j.postharvbio.2011.06.004 Casals C, Elmer PAG, Viñas I, Teixidó N, Sisquella M, Usall J (2012) The combination of curing with either chitosan or Bacillus subtilis CPA-8 to control brown rot infections caused by Monilinia fructicola. Postharvest Biol Tec 64:126-132

상세보기
Can J Microbiol F Cladera-Olivera 52 533 2006 10.1139/w05-159 Cladera-Olivera F, Caron GR, Motta AS, Souto AA, Brandelli A (2006) Bacteriocin-like substance inhibits potato soft rot caused by Erwinia carotovora. Can J Microbiol 52:533-539

상세보기
Biol Control DM Costa De 47 257 2008 10.1016/j.biocontrol.2008.08.010 De Costa DM, Zahra ARF, Kalpage MD, Rajapakse EMG (2008) Effectiveness and molecular characterization of Burkholderia spinosa, a prospective biocontrol agent for controlling postharvest disease of banana. Biol Control 47:257-267

상세보기
Biol Control BT Demoz 37 68 2006 10.1016/j.biocontrol.2005.11.010 Demoz BT, Korsten L (2006) Bacillus subtilis attachment, colonization, and survival on avocado flowers and its mode of action on stem-end rot pathogens. Biol Control 37:68-74

상세보기
Appl Environ Microb YH Dong 70 954 2004 10.1128/AEM.70.2.954-960.2004 Dong YH, Zhang XF, Xu JL, Zhang LH (2004) Insecticidal Bacillus thuringiensis silences Erwinia carotovora virulence by a new form of microbial antagonism, signal interference. Appl Environ Microb 70:954-960

상세보기
Postharvest Biol Tec S Droby 52 137 2009 10.1016/j.postharvbio.2008.11.009 Droby S, Wisniewski M, Macarisin D, Wilson C (2009) Twenty years of postharvest biocontrol research: is it time for a new paradigm? Postharvest Biol Tec 52:137-145

상세보기
Agric Sci China JH Du 6 214 2007 10.1016/S1671-2927(07)60037-6 Du JH, Fu MR, Li MM, Xia W (2007) Effects of chlorine dioxide gas on postharvest physiology and storage quality of green bell pepper (Capsicum frutescens L. var. Longrum). Agric Sci China 6:214-219

상세보기
Agric Sci China SY Fan 7 1181 2008 10.1016/S1671-2927(08)60162-5 Fan SY, Le JG, Cheng GJ, Wu CJ (2008) Chinese cabbage-pak-choi transcriptome map construction with cDNA-AFLP techniques. Agric Sci China 7:1181-1188

상세보기
Annu Rev Microbiol R Finking 58 453 2004 10.1146/annurev.micro.58.030603.123615 Finking R, Marahiel MA (2004) Biosynthesis of nonribosomal peptides. Annu Rev Microbiol 58:453-488

상세보기
Food and Drug Administration (1999) Code of Federal Regulations, Title 21: Food and Drugs, Chapter 1: Food and Drug Administration Department of Health and Human Services, Part 184: direct food substances affirmed as generally recognized as safe. US Government Printing Office, Washington, DC
Annals Bot M Heil 89 503 2002 10.1093/aob/mcf076 Heil M, Bostock RM (2002) Induced systemic resistance (ISR) against pathogens in the context of induced plant defences. Annals Bot 89:503-512

상세보기
Biol Control HQ Hu 54 359 2010 10.1016/j.biocontrol.2010.06.015 Hu HQ, Li XS, He H (2010) Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt. Biol Control 54:359-365

상세보기
Postharvest Biol Tec A Ippolito 19 265 2000 10.1016/S0925-5214(00)00104-6 Ippolito A, Ghaouth AE, Wilson CL, Wisniewski MA (2000) Control of postharvest decay of apple fruit by Aureobasidium pullulans and induction of defense responses. Postharvest Biol Tec 19:265-272

상세보기
Potato Research SH Jansky 52 57 2009 10.1007/s11540-008-9121-2 Jansky SH, Jin LP, Xie KY, Xie CH, Spooner DM (2009) Potato production and breeding in China. Potato Research 52:57-65

상세보기
J Gen Plant Pathol T Kikumoto 66 275 2000 10.1007/PL00012960 Kikumoto T (2000) Ecology and biocontrol of soft rot of Chinese cabbage. J Gen Plant Pathol 66:275-277

상세보기
J Food Protect CH Liao 72 85 2009 10.4315/0362-028X-72.1.85 Liao CH (2009) Control of foodborne pathogens and soft-rot bacteria on bell pepper by three strains of bacterial antagonists. J Food Protect 72:85-92

상세보기
Lett Appl Microbiol X Liu 51 30 2010 Liu X, Fang W, Liu L, Yu T, Lou B, Zheng X (2010) Biological control of postharvest sour rot of citrus by two antagonistic yeasts. Lett Appl Microbiol 51:30-35

상세보기
Appl Environ Microb LA Martin-Visscher 74 4756 2008 10.1128/AEM.00817-08 Martin-Visscher LA, van Belkum MJ, Garneau-Tsodikova S, Whittal RM, Zheng J, McMullen LM, Vederas JC (2008) Isolation and characterization of carnocyclin A, a novel circular bacteriocin produced by Carnobacterium maltaromaticum UAL307. Appl Environ Microb 74:4756-4763

상세보기
Postharvest Biol Tec MC Nally 64 40 2012 10.1016/j.postharvbio.2011.09.009 Nally MC, Pesce VM, Maturano YP, Muñoz CJ, Combina M, Toro ME, Castellanos de Figureoa LI, Vazquez F (2012) Biocontrol of Botrytis cinerea in table grapes by non-pathogenic indigenous Saccharomyces cerevisiae yeasts isolated from viticultural environments in Argentina. Postharvest Biol Tec 64:40-48

상세보기
Trends Microbiol M Ongena 16 115 2008 10.1016/j.tim.2007.12.009 Ongena M, Jacques P (2008) Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol 16:115-125

상세보기
Appl Microbiol Biot M Ongena 67 692 2005 10.1007/s00253-004-1741-0 Ongena M, Duby F, Jourdan E, Beaudry T, Jadin V, Dommes J, Thonart P (2005) Bacillus subtilis M4 decreases plant susceptibility towards fungal pathogens by increasing host resistance associated with differential gene expression. Appl Microbiol Biot 67:692-698

상세보기
Crop Prot MS Osman 30 173 2011 10.1016/j.cropro.2010.09.014 Osman MS, Sivakumar D, Korsten L (2011) Effect of biocontrol agent Bacillus amyloliquefaciens and 1-methyl cyclopropene on the control of postharvest diseases and maintenance of fruit quality. Crop Prot 30:173-178

상세보기
Plant Pathol MCM Pérombelon 51 1 2002 10.1046/j.0032-0862.2001.Short title.doc.x Pérombelon MCM (2002) Potato diseases caused by soft rot erwinias: an overview of pathogenesis. Plant Pathol 51:1-12

상세보기
Mol Plant Microbe In D Romero 20 430 2007 10.1094/MPMI-20-4-0430 Romero D, de Vicente A, Rakotoaly RH, Dufour SE, Veening JW, Arrebola E, Cazorla FM, Kuipers OP, Paquot M, Pérez-García A (2007) The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca. Mol Plant Microbe In 20:430-440

상세보기
Biol Control RR Sharma 50 205 2009 10.1016/j.biocontrol.2009.05.001 Sharma RR, Singh D, Singh R (2009) Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: a review. Biol Control 50:205-221

상세보기
10.1016/j.biocontrol.2012.06.002 Shu-Bin L, Mao F, Ren-Chao Z, Juan H, Xiao L (2012) Characterization and evaluation of the endophyte Bacillus B014 as a potential biocontrol agent for the control of Xanthomonas axonopodis pv. dieffenbachiae - induced blight of Anthurium. Biol Control. doi: http://dx.doi.org/10.1016/j.biocontrol.2012.06.002

상세보기
Mol Biol Evol K Tamura 28 2731 2011 10.1093/molbev/msr121 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731-2739

상세보기
Nucleic Acids Res JD Thompson 24 4876 1997 10.1093/nar/25.24.4876 Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876-4882
J Bacteriol WG Weisburg 173 697 1991 10.1128/jb.173.2.697-703.1991 Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697-703

상세보기
Appl Environ Microb S Yamamoto 61 1104 1995 10.1128/AEM.61.3.1104-1109.1995 Yamamoto S, Harayama S (1995) PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microb 61:1104-1109

상세보기
J Appl Microbiol HJ Yao 98 941 2005 10.1111/j.1365-2672.2004.02531.x Yao HJ, Tian SP (2005) Effects of a biocontrol agent and methyl jasmonate on postharvest diseases of peach fruit and the possible mechanisms involved. J Appl Microbiol 98:941-950

상세보기
Biol Control D Zhang 57 193 2011 10.1016/j.biocontrol.2011.02.011 Zhang D, Spadaro D, Garibaldi A, Gullino ML (2011) Potential biocontrol activity of a strain of Pichia guilliermondii against grey mold of apples and its possible modes of action. Biol Control 57:193-201

상세보기
Zhao B, He SJ (2002) Microbiology experiment. Science Press, Beijing. (In Chinese)
Phytopathology Y Zhao 102 252 2012 10.1094/PHYTO-06-11-0169 Zhao Y, Qian G, Fan J, Yin F, Zhou Y, Liu C, Shen Q, Hu B, Liu FQ (2012) Identification and characterization of a novel gene, hshB, in Xanthomonas oryzae pv. oryzicola co-regulated by quorum sensing and clp. Phytopathology 102:252-259

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Control of postharvest soft rot caused by Erwinia carotovora of vegetables by a strain of Bacillus amyloliquefaciens and its potential modes of action

Abstract ▼ AI-Helper

참고문헌 (38)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트