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Biotechnology for biofuels, v.7, 2014년, pp.111 -
Huang, Jinjin (State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, 2#,Yuanmingyuan West Road, Beijing, 100193 China) , Xia, Ji (State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, 2#,Yuanmingyuan West Road, Beijing, 100193 China) , Yang, Zhen (State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, 2#,Yuanmingyuan West Road, Beijing, 100193 China) , Guan, Feifei (Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, 5#, Panjiayuannanli Street, Beijing, 100021 China) , Cui, Di (State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, 2#,Yuanmingyuan West Road, Beijing, 100193 China) , Guan, Guohua (State Key Laboratories for Agro-biotechnology and College of Biological Sciences,) , Jiang, Wei , Li, Ying
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BackgroundWe previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the en...
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