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Construction of novel shuttle expression vectors for gene expression in Bacillus subtilis and Bacillus pumilus 원문보기

The Journal of general and applied microbiology, v.61 no.4, 2015년, pp.124 - 131  

Shao, Huanhuan (Key Laboratory of Bio-Resource & Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Sciences, Sichuan University) ,  Cao, Qinghua (Key Laboratory of Bio-Resource & Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Sciences, Sichuan University) ,  Zhao, Hongyan (Key Laboratory of Bio-Resource & Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Sciences, Sichuan University) ,  Tan, Xuemei (Key Laboratory of Bio-Resource & Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Sciences, Sichuan University) ,  Feng, Hong (Key Laboratory of Bio-Resource & Eco-Environment, Ministry of Education, Sichuan Key Laboratory of Molecular Biology & Biotechnology, College of Life Sciences, Sichuan University)

Abstract AI-Helper 아이콘AI-Helper

A native plasmid (pSU01) was detected by genome sequencing of Bacillus subtilis strain S1-4. Two pSU01-based shuttle expression vectors pSU02-AP and pSU03-AP were constructed enabling stable replication in B. subtilis WB600. These vectors contained the reporter gene aprE, encoding an alkaline protea...

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