[논문]Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells

Kim, S.Y.; Kim, Y.R.; Park, W.J.; Kim, H.S.; Jung, S.C.; Woo, S.Y.; Jo, I.; Ryu, K.H.; Park, J.W.

doi:10.1016/j.diff.2015.08.001

Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells

Differentiation, v.90 no.1/3, 2015년, pp.27 - 39

Kim, S.Y. , Kim, Y.R. , Park, W.J. , Kim, H.S. , Jung, S.C. , Woo, S.Y. , Jo, I. , Ryu, K.H. , Park, J.W.

Abstract ▼ AI-Helper

Tonsil-derived (T-) mesenchymal stem cells (MSCs) display mutilineage differentiation potential and self-renewal capacity and have potential as a banking source. Diabetes mellitus is a prevalent disease in modern society, and the transplantation of pancreatic progenitor cells or various stem cell-derived insulin-secreting cells has been suggested as a novel therapy for diabetes. The potential of T-MSCs to trans-differentiate into pancreatic progenitor cells or insulin-secreting cells has not yet been investigated. We examined the potential of human T-MSCs to trans-differentiate into pancreatic islet cells using two different methods based on β-mercaptoethanol and insulin-transferin-selenium, respectively. First, we compared the efficacy of the two methods for inducing differentiation into insulin-producing cells. We demonstrated that the insulin-transferin-selenium method is more efficient for inducing differentiation into insulin-secreting cells regardless of the source of the MSCs. Second, we compared the differentiation potential of two different MSC types: T-MSCs and adipose-derived MSCs (A-MSCs). T-MSCs had a differentiation capacity similar to that of A-MSCs and were capable of secreting insulin in response to glucose concentration. Islet-like clusters differentiated from T-MSCs had lower synaptotagmin-3, -5, -7, and -8 levels, and consequently lower secreted insulin levels than cells differentiated from A-MSCs. These results imply that T-MSCs can differentiate into functional pancreatic islet-like cells and could provide a novel, alternative cell therapy for diabetes mellitus.

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Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells

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Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells

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