[논문]Potassium aspartate inhibits SH-SY5Y cell damage and apoptosis induced by ouabain and H2O2

SUN, XUEFEI; MIN, DONGYU; WANG, YAN; HAO, LIYING

doi:10.3892/mmr.2015.3741

Potassium aspartate inhibits SH-SY5Y cell damage and apoptosis induced by ouabain and H2O2 원문보기

Molecular medicine reports, v.12 no.2, 2015년, pp.2842 - 2848

SUN, XUEFEI , MIN, DONGYU , WANG, YAN , HAO, LIYING

Abstract ▼ AI-Helper

The present study aimed to investigate the effects of L-aspartic acid potassium salt (potassium aspartate, K-asp) on SH-SY5Y cells treated with ouabain and H2O2. An 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to investigate the effects of K-asp on SH-SY5Y cell death induced by ouabain. Nissl staining was used to demonstrate the morphological changes of the SH-SY5Y cells. Light microscopy and 4',6-diamidino-2-phenylindole (DAPI) staining were performed to visualize apoptosis in SH-SY5Y cells incubated with ouabain for 6, 24 and 48 h. Transmission electron microscopy was used to observe the effect of K-asp on ultrastructural changes of the SH-SY5Y cells following incubation with ouabain for 24 and 48 h. An annexin V-fluorescein isothiocyanate/propidium binding assay and flow cytometry were performed successively to investigate how K-asp affected the H2O2-induced cell apoptosis. The MTT assay demonstrated that K-asp attenuated the cytotoxicity of the SH-SY5Y cells following treatment with ouabain, in a dose-dependent manner. The cell survival rates following 48 h incubation in the K-asp (15 mM) and K-asp (25 mM) groups were higher compared with the KCl and MK801 groups. Nissl staining demonstrated that the severity of cell injury in the KCl and K-asp (25 mM) groups were alleviated. In the DAPI staining and transmission electron microscopy analyses, KCl and K-asp (25 mM) reduced the rate of ouabain-induced apoptosis. Flow cytometry revealed that K-asp (25 mM) reduced H2O2 -induced apoptosis. These results demonstrated that K-asp (25 mM) inhibited the ouabain and H2O2-induced SH-SY5Y cell damage and apoptosis, possibly by supplementing levels of intracellular K(+).

참고문헌 (24)

Biol Pharm Bull Abe 23 1051 2000 10.1248/bpb.23.1051 Involvement of Na+-K+ pump in L-glutamate clearance by cultured rat cortical astrocytes

상세보기
Nihon Rinsho Ueno 12 250 2004 Sodium, potassium
Vet Clin North Am Small Anim Pract Willard 19 241 1989 10.1016/S0195-5616(89)50029-9 Disorders of potassium homeostasis

상세보기
J Neurol Neurosurg Psychiatry Shiino 75 1141 2004 10.1136/jnnp.2003.019943 Magnetic resonance spectroscopic determination of a neuronal and axonal marker in white matter predicts reversibility of deficits in secondary normal pressure hydrocephalus

상세보기
Am J Physiol Suleiman 272 H1063 1997 Changes in myocardial concentration of glutamate and aspartate during coronary artery surgery
J Biol Chem Saunders 286 27706 2011 10.1074/jbc.M110.213553 Isotopomer profiling of Leishmania mexicana promastigotes reveals important roles for succinate fermentation and aspartate uptake in tricarboxylic acid cycle (TCA) anaplerosis, glutamate synthesis and growth

상세보기
Annu Rev Neurosci Olanow 22 123 1999 10.1146/annurev.neuro.22.1.123 Etiology and pathogenesis of Parkinson’s disease

상세보기
Free Radic Biol Med Bellavite 4 225 1988 10.1016/0891-5849(88)90044-5 The superoxide-forming enzymatic system of phagocytes

상세보기
J Neurochem Halliwell 69 1330 1997 10.1046/j.1471-4159.1997.69031330.x Lipid peroxidation in brain homogenates: the role of iron and hydroxyl radicals

상세보기
Free Radic Biol Med Gardner 22 73 1997 10.1016/S0891-5849(96)00235-3 Apoptotic vs. nonapoptotic cytotoxicity induced by hydrogen peroxide

상세보기
Neuroscience Xiao 22 1350 2002 10.1523/JNEUROSCI.22-04-01350.2002 Ionic mechanism of ouabain induced concurrent apoptosis and necrosis in individual cultured cortical neurons

상세보기
Ann N Y Acad Sci Huang 1012 153 2004 10.1196/annals.1306.012 Redox-active metals, oxidative stress and Alzheimer’s disease pathology

상세보기
Biochim Biophys Acta Kulikov 1768 1691 2007 10.1016/j.bbamem.2007.04.012 Ouabain activates signaling pathways associated with cell death in human neuroblastoma

상세보기
Science Yu 284 336 1999 10.1126/science.284.5412.336 NMDA receptor mediated K+ efflux and neuronal apoptosis

상세보기
J Cell Sci Wang 116 2099 2003 10.1242/jcs.00420 Apoptotic insults impair Na+-K+-ATPase activity as a mechanism of neuronal death mediated by concurrent ATP deficiency and oxidant stress

상세보기
Brain Res Xiao 955 253 2002 10.1016/S0006-8993(02)03472-8 Slight impairment of Na+-K+-ATPase synergistically aggravates ceramide- and beta-amyloid-induced apoptosis in cortical neurons

상세보기
J Physiol Yurinskaya 589 2197 2011 10.1113/jphysiol.2011.207571 Balance of unidirectional monovalent ion fluxes in cells undergoing apoptosis: why does Na+-K+ pump suppression not cause cell swelling?

상세보기
Histol Histopathol Brevet 23 965 2008 Expression of K+ channels in normal and cancerous human breast
Neuroscience Hernandez-Enriquez 167 298 2010 10.1016/j.neuroscience.2010.01.046 Role for ionic fluxes on cell death and apoptotic volume decrease in cultured cerebellar granule neurons

상세보기
Biol Pharm Bull Huang 35 449 2012 10.1248/bpb.35.449 Astragalus extract alleviates nerve injury after cerebral ischemia by improving energy metabolism and inhibiting apoptosis

상세보기
Neurosci Lett Wang 487 383 2011 10.1016/j.neulet.2010.10.061 In vitro effect of altering potassium concentration in artificial endolymph on apoptosis and ultrastructure features of olfactory bulb neural precursor cells

상세보기
Biochim Biophys Acta Jorgensen 1505 57 2001 10.1016/S0005-2728(00)00277-2 Structure-function relationships of Na+, K+, ATP, or Mg2+ binding and energy transduction in Na, K-ATPase

상세보기
Cell Mol Biol Lett Gugliotta 17 586 2012 10.2478/s11658-012-0027-2 Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

상세보기
Biol Pharm Bull Noguchi 33 2013 2010 10.1248/bpb.33.2013 Modulation of voltage-gated ion channels on SH-SY5Y neuroblastoma by non-ionic surfactant, Cremophor EL

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Potassium aspartate inhibits SH-SY5Y cell damage and apoptosis induced by ouabain and H2O2 원문보기

Abstract ▼ AI-Helper

참고문헌 (24)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트