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International journal of cosmetic science, v.38 no.3, 2016년, pp.319 - 324
Jhan, J.‐K. (Department of Food and Nutrition, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung City, 43301, Taiwan, ROC) , Chung, Y.‐C. (Department of Food and Nutrition, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung City, 43301, Taiwan, ROC) , Chen, G.‐H. (Department of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung City, 43301, Taiwan, ROC) , Chang, C.‐H. (Department of Food Science, National Chiayi University, 300 Syuefu Rd, Chiayi City, 60004, Taiwan, ROC) , Lu, Y.‐C. (Department of Food Science, National Chiayi University, 300 Syuefu Rd, Chiayi City, 60004, Taiwan, ROC) , Hsu, C.‐K. (Department of Food Science, National Chiayi University, 300 Syuefu Rd, Chiayi City, 60004, Taiwan, ROC)
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AbstractObjectiveThe seed coat of black soya bean (SCBS) contains high amount of anthocyanins and shows antioxidant and anti‐mushroom tyrosinase activities. The objectives of this study were to analyse the anthocyanins in SCBS with different solvents and to find the relationship between anthoc...
RésuméObjectifL'enveloppe de la graine du soja noir (SCBS) contient une quantité élevée des anthocyanes et possèdent des activités antioxydantes et anti‐tyrosinase de champignon. Les objectifs de cette étude étaient d'analyser les anthocyanes dans SCBS avec différents solvants et de trouver la relation entre le profil des anthocyanes avec les activités anti‐tyrosinase humaine et champignon.MéthodesSCBS a été extrait à l'eau chaude, à l'éthanol à 50 et 80%, à l'acétone 50 et 80% et à l' d'acétone acidifiée à 50 and 80%. Les phénols et le total des flavonoïdes contenus dans les extraits ont été déterminés. Les Anthocyanins dans les extraits ont été analysées par HPLC et LC / MS / MS. La tyrosinase humaine obtenue par génie génétique a été utilisée pour évaluer le potentiel anti‐tyrosinase des extraits de SCBS.ResultatsL'extrait à l'acétone 80% à partir du SCBS a montré la plus haute teneur en phénols, en flavonoïdes, et en cyanidine‐3‐O‐glucoside (C3G) entre tous les extraits, alors que l'extrait à l'eau chaude a montré les plus basses concentrations en antioxydants. Trois composés anthocyanes ont été trouvés dans tous les extraits de SCBS, et l'analyse de HPLC et LC / MS / MS a indiqué qu'ils représentent C3G, delphinidine‐3‐O‐glucoside (D3G) et péonidine‐3‐O‐glucoside (P3G). Les rapports des C3G (2,84 mg / g), D3G (0,34 mg / g) et de P3G (0,35 mg / g) dans l'extrait 80% d'acétone acidifié étaient de 76,6, 9,1 et 9,3%, respectivement. Tous les extraits de SCBS possédaient une activité anti‐tyrosinase humaine. En outre, une bonne corrélation a été trouvée entre les activités anti‐tyrosinase humaine et les contenus C3G dans les extraits.ConclusionLes antioxydants dans SCBS possèdent des deux activités anti‐tyrosinase humaine et champignon.
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