[논문]High throughput bioassay for beta1-adrenoceptor autoantibody detection

Joshi-Barr, S.; Haberland, A.; Bartel, S.; Muller, J.; Choi, T.; Wallukat, G.

doi:10.1016/j.ijcard.2016.06.002

High throughput bioassay for beta1-adrenoceptor autoantibody detection

International journal of cardiology, v.219, 2016년, pp.98 - 104

Joshi-Barr, S. , Haberland, A. , Bartel, S. , Muller, J. , Choi, T. , Wallukat, G.

Abstract ▼ AI-Helper

Background: While the involvement of adrenergic beta1-autoantibodies (beta1-AABs) in pathogenesis of cardiomyopathies is well established as are the benefits associated with autoantibody removal by immunoapheresis, the development of drugs neutralizing beta1-AABs in-vivo has been slowed due to a lack of high throughput autoantibody analytics. Highly scalable routine diagnostics involving immobilized binding partners have mostly failed in comparison to the laborious bioassays, which are difficult to scale up, but present the most reliable and sensitive tools for detecting the beta1-autoantibodies. Methods: A high throughput, image-based assay to measure cardiomyocyte beat rate and contractility was developed and tested for its applicability for detecting adrenergic beta1-autoantibodies. The classical bioassay of spontaneously beating neonatal rat cardiomyocytes was used for comparison. Results: The high throughout assay using human iPSC-derived cardiomyocytes was able to detect beta1-AAB activity of biological sample material. The results from the high throughput assay were very similar to the data obtained from the original bioassay of spontaneously beating neonatal cardiomyocytes, with one exception, where a control antibody targeting the N-terminal end of the human beta1-receptor induced a response when tested with the high throughput imager, while none was observed by the classical bioassay. This discrepancy may be explained by the differences in host species of cardiomyocytes tested by the two methods. Conclusion: The high throughput system using iPSC-derived cardiomyocytes for the detection of beta1-AAB provides a realistic option to overcome the sample-size limitations of the bioassay-based diagnostics.

주제어

참고문헌 (16)

Weigner
Circulation Muller S. 385 2000 10.1161/01.CIR.101.4.385 Immunoglobulin adsorption in patients with idiopathic dilated cardiomyopathy

상세보기
Eur. J. Heart Fail. Dandel 14 12 S. 1374 2012 10.1093/eurjhf/hfs123 Long-term benefits of immunoadsorption in β(1)-adrenoceptor autoantibody-positive transplant candidates with dilated cardiomyopathy

상세보기
Atheroscler. Suppl. Dandel 1 S. 203 2013 10.1016/j.atherosclerosissup.2012.10.029 Immunoadsorption therapy for dilated cardiomyopathy and pulmonary arterial hypertension

상세보기
Atheroscler. Suppl. Dandel S. 124 2015 10.1016/j.atherosclerosissup.2015.02.023 Immunoadsorption can improve cardiac function in transplant candidates with non-ischemic dilated cardiomyopathy associated with diabetes mellitus

상세보기
Circ. J. Baba 74 7 S. 1372 2010 10.1253/circj.CJ-09-0748 Complete elimination of cardiodepressant IgG3 autoantibodies by immunoadsorption in patients with severe heart failure

상세보기
Eur. J. Heart Fail. Patel 7 S. 724 2013 10.1093/eurjhf/hft065 Targeting anti-beta-1-adrenergic receptor antibodies for dilated cardiomyopathy

상세보기
Autoimmun. Rev. Bornholz 9 S. 954 2014 10.1016/j.autrev.2014.08.021 Diagnostic and therapeutic aspects of β1-adrenergic receptor autoantibodies in human heart disease

상세보기
Cardiology Jahns 131 3 S. 49 2015 10.1159/000376546 Questionable validity of peptide-based ELISA strategies in the diagnostics of cardiopathogenic autoantibodies that activate G-protein-coupled receptors

상세보기
Biomed. Biochim. Acta Wallukat 46 8-9 S. S634 1987 Effects of the serum gamma globulin fraction of patients with allergic asthma and dilated cardiomyopathy on chronotropic beta adrenoceptor function in cultured neonatal rat heart myocytes
J. Mol. Cell. Cardiol. Wallukat 11 S. 2529 1995 Anti-beta 1-adrenoceptor autoantibodies with chronotropic activity from the serum of patients with dilated cardiomyopathy: mapping of epitopes in the first and second extracellular loops
J. Am. Coll. Cardiol. Wallukat 55 5 S. 463 22010 10.1016/j.jacc.2009.06.064 Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas' cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas' patients

상세보기
Expert. Rev. Clin. Immunol. Xia 5 S. 659 2011 10.1586/eci.11.56 Receptor-activating autoantibodies and disease: preeclampsia and beyond

상세보기
Int. J. Cardiol. Bornholz Bd. 202 S. 728 2016 10.1016/j.ijcard.2015.10.068 Detection of DCM-associated β1-adrenergic receptor autoantibodies requires functional readouts or native human β1-receptors as targets

상세보기
Cardiovasc. Res. Sterin-Borda Bd. 10, 6 S. 613 1976 10.1093/cvr/10.6.613 Effect of chagasic sera on the rat isolated atrial preparation: immunological, morphological and function aspects

상세보기
J. Am. Coll. Cardiol. Nikolaev Bd. 50, 5 S. 423 2007 10.1016/j.jacc.2007.03.051 A novel fluorescence method for the rapid detection of functional beta1-adrenergic receptor autoantibodies in heart failure

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

High throughput bioassay for beta1-adrenoceptor autoantibody detection

Abstract ▼ AI-Helper

주제어

참고문헌 (16)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트