[논문]Identification and quantification of microplastics using Nile Red staining

Shim, Won Joon; Song, Young Kyoung; Hong, Sang Hee; Jang, Mi

doi:10.1016/j.marpolbul.2016.10.049

[해외논문] Identification and quantification of microplastics using Nile Red staining

Marine pollution bulletin, v.113 no.1/2, 2016년, pp.469 - 476

Shim, Won Joon (Corresponding author at: Oil and POPs Research Group, Korea Institute of Ocean Science and Technology.) , Song, Young Kyoung , Hong, Sang Hee , Jang, Mi

Abstract ▼ AI-Helper

We investigated the applicability of Nile Red (NR), a fluorescent dye, for microplastic analysis, and determined the optimal staining conditions. Five mg/L NR solution in n-hexane effectively stained plastics, and they were easily recognized in green fluorescence. The NR staining method was successfully applied to micro-sized polyethylene, polypropylene, polystyrene, polycarbonate, polyurethane, and poly(ethylene-vinyl acetate), except for polyvinylchloride, polyamide and polyester. The recovery rate of polyethylene (100-300 pm) spiked to pretreated natural sand was 98% in the NR stating method, which was not significantly (p < 0.05) different with FT-IR identification. The NR staining method was suitable for discriminating fragmented polypropylene particles from large numbers of sand particles in laboratory weathering test samples. The method is straightforward and quick for identifying and quantifying polymer particles in the laboratory controlled samples. Further studies, however, are necessary to investigate the application of NR staining to field samples with organic remnants. (C) 2016 Elsevier Ltd. All rights reserved.

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[해외논문] Identification and quantification of microplastics using Nile Red staining

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[해외논문] Identification and quantification of microplastics using Nile Red staining

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