$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis 원문보기

Scientific reports, v.7, 2017년, pp.43306 -   

Leclercq, Sophie (Centre d’Ingé) ,  Derouaux, Adeline (nierie des Proté) ,  Olatunji, Samir (ines, University of Liè) ,  Fraipont, Claudine (ge, B6a, Quartier Agora , allé) ,  Egan, Alexander J. F. (e du six Aoû) ,  Vollmer, Waldemar (t 11, 4000 Liè) ,  Breukink, Eefjan (ge 1, Belgium) ,  Terrak, Mohammed (Centre d’Ingé)

Abstract AI-Helper 아이콘AI-Helper

Bacteria utilize specialized multi-protein machineries to synthesize the essential peptidoglycan (PG) cell wall during growth and division. The divisome controls septal PG synthesis and separation of daughter cells. In E. coli, the lipid II transporter candidate FtsW is thought to work in concert wi...

참고문헌 (56)

  1. Vollmer W. , Blanot D. & de Pedro M. A. Peptidoglycan structure and architecture . FEMS Microbiol Rev 32 , 149 – 167 ( 2008 ). 18194336 

  2. Egan A. J. F. & Vollmer W. The physiology of bacterial cell division . Ann N Y Acad Sci 1277 , 8 – 28 ( 2013 ). 23215820 

  3. Mohammadi T. . Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane . EMBO J 30 , 1425 – 32 ( 2011 ). 21386816 

  4. Sham L.-T. . Bacterial cell wall. MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis . Science 345 , 220 – 2 ( 2014 ). 25013077 

  5. Ruiz N. Lipid Flippases for Bacterial Peptidoglycan Biosynthesis . Lipid Insights 8 , 21 – 31 ( 2015 ). 26792999 

  6. Sauvage E. & Terrak M. Glycosyltransferases and Transpeptidases/Penicillin-Binding Proteins: Valuable Targets for New Antibacterials . Antibitics 5 , 12 ( 2016 ). 

  7. Sauvage E. , Kerff F. F. , Terrak M. , Ayala J. A. & Charlier P. The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis . FEMS Microbiol Rev 32 , 234 – 258 ( 2008 ). 18266856 

  8. Gray A. N. . Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division . Elife 4 ( 2015 ). 

  9. Gerding M. A. , Ogata Y. , Pecora N. D. , Niki H. & de Boer P. A. J. The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli . Mol Microbiol 63 , 1008 – 25 ( 2007 ). 17233825 

  10. Aarsman M. E. . Maturation of the Escherichia coli divisome occurs in two steps . Mol Microbiol 55 , 1631 – 1645 ( 2005 ). 15752189 

  11. Buddelmeijer N. & Beckwith J. A complex of the Escherichia coli cell division proteins FtsL, FtsB and FtsQ forms independently of its localization to the septal region . Mol Microbiol 52 , 1315 – 1327 ( 2004 ). 15165235 

  12. Fraipont C. . The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli . Microbiology 157 , 251 – 9 ( 2011 ). 20847002 

  13. Goehring N. W. , Gonzalez M. D. & Beckwith J. Premature targeting of cell division proteins to midcell reveals hierarchies of protein interactions involved in divisome assembly . Mol Microbiol 61 , 33 – 45 ( 2006 ). 16824093 

  14. Mercer K. L. N. & Weiss D. S. The Escherichia coli cell division protein FtsW is required to recruit its cognate transpeptidase, FtsI (PBP3), to the division site . J Bacteriol 184 , 904 – 12 ( 2002 ). 11807049 

  15. Bertsche U. . Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli . Mol Microbiol 61 , 675 – 690 ( 2006 ). 16803586 

  16. Müller P. . The Essential Cell Division Protein FtsN Interacts with the Murein (Peptidoglycan) Synthase PBP1B in Escherichia coli . J Biol Chem 282 , 36394 – 36402 ( 2007 ). 17938168 

  17. Mohammadi T. . Specificity of the transport of lipid II by FtsW in Escherichia coli . J Biol Chem 289 , 14707 – 18 ( 2014 ). 24711460 

  18. Pastoret S. . Functional analysis of the cell division protein FtsW of Escherichia coli . J Bacteriol 186 , 8370 – 8379 ( 2004 ). 15576787 

  19. Hvorup R. N. . The multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily . Eur J Biochem 270 , 799 – 813 ( 2003 ). 12603313 

  20. Elhenawy W. . The O-Antigen Flippase Wzk Can Substitute for MurJ in Peptidoglycan Synthesis in Helicobacter pylori and Escherichia coli . PLoS One 11 , e0161587 ( 2016 ). 27537185 

  21. Meeske A. J. . MurJ and a novel lipid II flippase are required for cell wall biogenesis in Bacillus subtilis . Proc Natl Acad Sci USA 112 , 6437 – 42 ( 2015 ). 25918422 

  22. Meeske A. J. . SEDS proteins are a widespread family of bacterial cell wall polymerases . Nature 537 , 634 – 638 ( 2016 ). 27525505 

  23. Paradis-Bleau C. . Lipoprotein cofactors located in the outer membrane activate bacterial cell wall polymerases . Cell 143 , 1110 – 20 ( 2010 ). 21183074 

  24. Typas A. . Regulation of peptidoglycan synthesis by outer-membrane proteins . Cell 143 , 1097 – 109 ( 2010 ). 21183073 

  25. Egan A. J. F. . Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B . Proc Natl Acad Sci USA 111 , 8197 – 202 ( 2014 ). 24821816 

  26. Liu B. , Persons L. , Lee L. & de Boer P. A. J. Roles for both FtsA and the FtsBLQ subcomplex in FtsN-stimulated cell constriction in Escherichia coli . Mol Microbiol 95 , 945 – 70 ( 2015 ). 25496160 

  27. Tsang M.-J. & Bernhardt T. G. A role for the FtsQLB complex in cytokinetic ring activation revealed by an ftsL allele that accelerates division . Mol Microbiol 95 , 925 – 44 ( 2015 ). 25496050 

  28. Trip E. N. & Scheffers D.-J. A 1 MDa protein complex containing critical components of the Escherichia coli divisome . Sci Rep 5 , 18190 ( 2015 ). 26643979 

  29. Noirclerc-Savoye M. . Reconstitution of membrane protein complexes involved in pneumococcal septal cell wall assembly . PLoS One 8 , e75522 ( 2013 ). 24147156 

  30. Di Lallo G. , Fagioli M. , Barionovi D. , Ghelardini P. & Paolozzi L. Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation . Microbiology 149 , 3353 – 9 ( 2003 ). 14663069 

  31. Karimova G. , Dautin N. & Ladant D. Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis . J Bacteriol 187 , 2233 – 43 ( 2005 ). 15774864 

  32. Piette A. . Structural determinants required to target penicillin-binding protein 3 to the septum of Escherichia coli . J Bacteriol 186 , 6110 – 7 ( 2004 ). 15342580 

  33. Wissel M. C. , Wendt J. L. , Mitchell C. J. & Weiss D. S. The transmembrane helix of the Escherichia coli division protein FtsI localizes to the septal ring . J Bacteriol 187 , 320 – 8 ( 2005 ). 15601716 

  34. Ovchinnikov S. . Large scale determination of previously unsolved protein structures using evolutionary information . Elife 4 , e09248 ( 2015 ). 26335199 

  35. Khattar M. M. . Two polypeptide products of the Escherichia coli cell division gene ftsW and a possible role for FtsW in FtsZ function . J Bacteriol 179 , 784 – 93 ( 1997 ). 9006034 

  36. Schwartz B. , Markwalder J. A. , Seitz S. P. , Wang Y. & Stein R. L. A kinetic characterization of the glycosyltransferase activity of Eschericia coli PBP1b and development of a continuous fluorescence assay . Biochemistry 41 , 12552 – 12561 ( 2002 ). 12369847 

  37. Egan A. J. F. & Vollmer W. Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases . Methods Mol Biol 1440 , 171 – 84 ( 2016 ). 27311672 

  38. Bertsche U. , Breukink E. , Kast T. & Vollmer W. In vitro murein peptidoglycan synthesis by dimers of the bifunctional transglycosylase-transpeptidase PBP1B from Escherichia coli . J Biol Chem 280 , 38096 – 38101 ( 2005 ). 16154998 

  39. Born P. , Breukink E. & Vollmer W. In vitro synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli . J Biol Chem 281 , 26985 – 26993 ( 2006 ). 16840781 

  40. Egan A. J. F. , Biboy J. , van’t Veer I. , Breukink E. & Vollmer W. Activities and regulation of peptidoglycan synthases . Philos Trans R Soc B Biol Sci 370 , 20150031 ( 2015 ). 

  41. Khattar M. M. , Begg K. J. & Donachie W. D. Identification of FtsW and characterization of a new ftsW division mutant of Escherichia coli . J Bacteriol 176 , 7140 – 7 ( 1994 ). 7961485 

  42. Cho H. . Bacterial cell wall biogenesis is mediated by SEDS and PBP polymerase families functioning semi-autonomously . Nat Microbiol 1 , 16172 ( 2016 ). 

  43. Höltje J. V. Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli . Microbiol Mol Biol Rev 62 , 181 – 203 ( 1998 ). 9529891 

  44. Banzhaf M. . Cooperativity of peptidoglycan synthases active in bacterial cell elongation . Mol Microbiol 85 , 179 – 194 ( 2012 ). 22606933 

  45. Yousif S. Y. , Broome-Smith J. K. & Spratt B. G. Lysis of Escherichia coli by beta-lactam antibiotics: deletion analysis of the role of penicillin-binding proteins 1A and 1B . J Gen Microbiol 131 , 2839 – 45 ( 1985 ). 3906031 

  46. Reed P. , Veiga H. , Jorge A. M. , Terrak M. & Pinho M. G. Monofunctional transglycosylases are not essential for Staphylococcus aureus cell wall synthesis . J Bacteriol 193 , 2549 – 2556 ( 2011 ). 21441517 

  47. Datta P. . Interaction between FtsW and penicillin-binding protein 3 (PBP3) directs PBP3 to mid-cell, controls cell septation and mediates the formation of a trimeric complex involving FtsZ, FtsW and PBP3 in mycobacteria . Mol Microbiol 62 , 1655 – 73 ( 2006 ). 17427288 

  48. Matsumoto S. . Crystal structures of an archaeal oligosaccharyltransferase provide insights into the catalytic cycle of N-linked protein glycosylation . Proc Natl Acad Sci USA 110 , 17868 – 73 ( 2013 ). 24127570 

  49. Petrou V. I. . Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation . Science (80-) 351 , 608 – 612 ( 2016 ). 

  50. Terrak M. . The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan-polymerizing penicillin-binding protein 1b of Escherichia coli . Mol Microbiol 34 , 350 – 364 ( 1999 ). 10564478 

  51. Breukink E. . Lipid II is an intrinsic component of the pore induced by nisin in bacterial membranes . J Biol Chem 278 , 19898 – 19903 ( 2003 ). 12663672 

  52. Lakaye B. . Synthesis, purification and kinetic properties of fluorescein-labelled penicillins . Biochem J 300 Pt 1, 141 – 145 ( 1994 ). 8198525 

  53. Barrett D. . Analysis of glycan polymers produced by peptidoglycan glycosyltransferases . J Biol Chem 282 , 31964 – 31971 ( 2007 ). 17704540 

  54. Biboy J. , Bui N. K. & Vollmer W. In vitro peptidoglycan synthesis assay with lipid II substrate . Methods Mol Biol 966 , 273 – 88 ( 2013 ). 23299741 

  55. Gallagher S. R. One-dimensional electrophoresis using nondenaturing conditions . Curr Protoc Mol Biol Chapter 10 , Unit 10.2B ( 2001 ). 

  56. Miroux B. & Walker J. E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels . J Mol Biol 260 , 289 – 298 ( 1996 ). 8757792 

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

맨위로