[논문]Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody

Liu, Jianbo; Shi, Hongyan; Chen, Jianfei; Zhang, Xin; Ji, Zhaoyang; Yuan, Jing; Shi, Da; Cao, Liyan; Zhu, Xiangdong; Dong, Hui; Wang, Xiaobo; Zhang, Jialin; Feng, Li

doi:10.1016/j.virol.2017.04.026

Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody 원문보기

Virology, v.507, 2017년, pp.257 - 262

Liu, Jianbo (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China) , Shi, Hongyan (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China) , Chen, Jianfei (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China) , Zhang, Xin (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China) , Ji, Zhaoyang (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China) , Yuan, Jing (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China) , Shi, Da (State Key Laboratory of Veterinary Biotechnolo) , Cao, Liyan , Zhu, Xiangdong , Dong, Hui , Wang, Xiaobo , Zhang, Jialin , Feng, Li

Abstract ▼ AI-Helper

Abstract Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutralized new variant genotype 2 PEDV strains (LNCT2, LNSY, and Hjms), but it did not neutralize a genotype 1 PEDV strain (CV777), in vitro. Results showed that the epitope recognized by the 1B9 mAb lies in the spike protein, and that it is a conformational epitope. These findings confirm that allelic differences in the PEDV S gene between the G1 and G2 genotype strains led to changes in the S protein and, thus, differences in its immunogenicity. Highlights The mAb of 1B9 could neutralize PEDV new variant strains from genotype 2. The mAb of 1B9 could not neutralize PEDV strain from genotype 1. The antiviral strategy of mAb 1B9 work by combination with the S protein of PEDV. The epitope recognized by mAb 1B9 is a conformational epitope.

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Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody 원문보기

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