[논문]Major depletion of SOX2 + stem cells in the adult pituitary is not restored which does not affect hormonal cell homeostasis and remodelling

Roose, Heleen; Cox, Benoit; Boretto, Matteo; Gysemans, Conny; Vennekens, Annelies; Vankelecom, Hugo

doi:10.1038/s41598-017-16796-2

Major depletion of SOX2 + stem cells in the adult pituitary is not restored which does not affect hormonal cell homeostasis and remodelling 원문보기

Scientific reports, v.7, 2017년, pp.16940 -

Roose, Heleen (Department of Development and Regeneration, Cluster of Stem Cell and Developmental Biology (SCDB), Unit of Stem Cell Research, KU Leuven (University of Leuven), Leuven, Belgium) , Cox, Benoit (Department of Development and Regeneration, Cluster of Stem Cell and Developmental Biology (SCDB), Unit of Stem Cell Research, KU Leuven (University of Leuven), Leuven, Belgium) , Boretto, Matteo (Department of Development and Regeneration, Cluster of Stem Cell and Developmental Biology (SCDB), Unit of Stem Cell Research, KU Leuven (University of Leuven), Leuven, Belgium) , Gysemans, Conny (Department of Chronic Diseases, Metabolism and Ageing, Clinical and Experimental Endocrinology, KU Leuven (University of Leuven), Leuven, Belgium) , Vennekens, Annelies (Department of Development and Regeneration, Cluster of Stem Cell and Developmental Biology (SCDB), Unit of Stem Cell Research, KU Leuven (University of Leuven), Leuven, Belgium) , Vankelecom, Hugo (Department of Development and Regeneration, Cluster of Stem Cell and Developmental Biology (SCDB), Unit of Stem Cell Research, KU Leuven)

Abstract ▼ AI-Helper

The pituitary gland contains SOX2-expressing stem cells. However, their functional significance remains largely unmapped. We investigated their importance by depleting SOX2+ cells through diphtheria toxin (DT)-mediated ablation. DT treatment of adult Sox2CreERT2/+;R26iDTR/+ mice (after tamoxifen-induced expression of DT receptor in SOX2+ cells) resulted in 80% obliteration of SOX2+ cells in the endocrine pituitary, coinciding with reduced pituisphere-forming activity. Counterintuitively for a stem cell population, the SOX2+ cell compartment did not repopulate. Considering the more active phenotype of the stem cells during early-postnatal pituitary maturation, SOX2+ cell ablation was also performed in 4- and 1-week-old animals. Ablation grade diminished with decreasing age and was accompanied by a proliferative reaction of the SOX2+ cells, suggesting a rescue attempt. Despite this activation, SOX2+ cells did also not recover. Finally, the major SOX2+ cell depletion in adult mice did not affect the homeostatic maintenance of pituitary hormonal cell populations, nor the corticotrope remodelling response to adrenalectomy challenge. Taken together, our study shows that pituitary SOX2+ fail to regenerate after major depletion which does not affect adult endocrine cell homeostasis and remodelling. Thus, pituitary SOX2+ cells may constitute a copious stem cell reserve or may have other critical role(s) still to be clearly defined.

참고문헌 (29)

1. Vankelecom H Gremeaux L Stem cells in the pituitary gland: A burgeoning field Gen. Comp. Endocrinol. 2010 166 478 488 10.1016/j.ygcen.2009.11.007 19917287

상세보기
2. Fauquier T Rizzoti K Dattani M Lovell-Badge R Robinson ICAF SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland Proc. Natl. Acad. Sci. USA 2008 105 2907 2912 10.1073/pnas.0707886105 18287078

상세보기
3. Chen J Pituitary progenitor cells tracked down by side population dissection Stem Cells 2009 27 1182 1195 10.1002/stem.51 19418455

상세보기
4. Arnold K Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice Cell Stem Cell 2011 9 317 329 10.1016/j.stem.2011.09.001 21982232

상세보기
5. Sarkar A Hochedlinger K The Sox family of transcription factors : versatile regulators of stem and progenitor cell fate. Stem Cell 2013 12 15 30
6. Fu Q Vankelecom H Regenerative capacity of the adult pituitary: multiple mechanisms of lactotrope restoration after transgenic ablation Stem Cells Dev. 2012 21 3245 3257 10.1089/scd.2012.0290 22970885

상세보기
7. Fu Q The adult pituitary shows stem/progenitor cell activation in response to injury and is capable of regeneration Endocrinology 2012 153 3224 3235 10.1210/en.2012-1152 22518061

상세보기
8. Willems C Regeneration in the pituitary after cell-ablation injury: time-related aspects and molecular analysis Endocrinology 2016 157 705 721 10.1210/en.2015-1741 26653762

상세보기
9. Andoniadou CL Sox2(+) stem/progenitor cells in the adult mouse pituitary support organ homeostasis and have tumor-inducing potential Cell Stem Cell 2013 13 433 445 10.1016/j.stem.2013.07.004 24094324

상세보기
10. Rizzoti K Akiyama H Lovell-Badge R Mobilized adult pituitary stem cells contribute to endocrine regeneration in response to physiological demand Cell Stem Cell 2013 13 419 432 10.1016/j.stem.2013.07.006 24094323

상세보기
11. Gaston-Massuet C Increased Wingless (Wnt) signaling in pituitary progenitor/stem cells gives rise to pituitary tumors in mice and humans Proc. Natl. Acad. Sci. USA 2011 108 11482 11487 10.1073/pnas.1101553108 21636786

상세보기
12. Garcia-Lavandeira M A GRFa2/Prop1/stem (GPS) cell niche in the pituitary PLoS One 2009 4 e4815 10.1371/journal.pone.0004815 19283075

상세보기
13. Chen M Coxsackievirus and adenovirus receptor-positive cells compose the putative stem/progenitor cell niches in the marginal cell layer and parenchyma of the rat anterior pituitary Cell Tissue Res. 2013 354 823 836 10.1007/s00441-013-1713-8 24057874

상세보기
14. Carbajo-Pérez E Watanabe YG Cellular proliferation in the anterior pituitary of the rat during the postnatal period Cell Tissue Res. 1990 261 333 338 10.1007/BF00318674 2401005

상세보기
15. Taniguchi Y Yasutaka S Kominami R Shinohara H Proliferation and differentiation of rat anterior pituitary cells Anat. Embryol. 2002 206 1 11 10.1007/s00429-002-0271-8 12478362

상세보기
16. Gremeaux L Fu Q Chen J Vankelecom H Activated phenotype of the pituitary stem/progenitor cell compartment during the early-postnatal maturation phase of the gland Stem Cells Dev. 2012 21 801 813 10.1089/scd.2011.0496 21970375

상세보기
17. Nolan L Levy A A population of non-luteinising hormone/non-adrenocorticotrophic hormone-positive cells in the male rat anterior pituitary responds mitotically to both gonadectomy and adrenalectomy J Neuroendocr. 2006 18 655 661 10.1111/j.1365-2826.2006.01459.x

상세보기
18. Blaas L Lgr6 labels a rare population of mammary gland progenitor cells that are able to originate luminal mammary tumours Nat. Cell Biol. 2016 18 1346 1356 10.1038/ncb3434 27798604

상세보기
19. Hoeck JD Stem cell plasticity enables hair regeneration following Lgr5+ cell loss Nat. Cell Biol. 2017 19 666 676 10.1038/ncb3535 28553937

상세보기
20. Zhang Y Hypothalamic stem cells control ageing speed partly through exosomal miRNAs Nature 2017 548 52 57 10.1038/nature23282 28746310

상세보기
21. Tian H A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable Nature 2011 478 255 259 10.1038/nature10408 21927002

상세보기
22. Yan K Chia L Li X The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations Pnas 2012 109 466 471 10.1073/pnas.1118857109 22190486

상세보기
23. Vallone VF Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Development 2016 143 1452 1463 10.1242/dev.131490 26989172

상세보기
24. Ito M Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis Nat. Med. 2005 11 1351 1354 10.1038/nm1328 16288281

상세보기
25. Huch M In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration Nature 2013 494 247 250 10.1038/nature11826 23354049

상세보기
26. Chen J The adult pituitary contains a cell population displaying stem/progenitor cell and early embryonic characteristics Endocrinology 2005 146 3985 3998 10.1210/en.2005-0185 15932930

상세보기
27. Mertens F Pituitary tumors contain a side population with tumor stem cell-associated characteristics Endocr. Relat. Cancer 2015 22 481 504 10.1530/ERC-14-0546 25921430

상세보기
28. Boretto M Development of organoids from mouse and human endometrium showing endometrial epithelium physiology and long-term expandability Development 2017 144 1775 1786 10.1242/dev.148478 28442471

상세보기
29. Marshall OJ PerlPrimer: Cross-platform, graphical primer design for standard, bisulphite and real-time PCR Bioinformatics 2004 20 2471 2472 10.1093/bioinformatics/bth254 15073005

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Major depletion of SOX2 + stem cells in the adult pituitary is not restored which does not affect hormonal cell homeostasis and remodelling 원문보기

Abstract ▼ AI-Helper

참고문헌 (29)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트